ABSTRACT
The cauliflower mosaic virus (CaMV) hybrid S delta II, partially deleted in ORFII, loses its transmissibility by the aphid Myzus persicae on 5-min acquisition feed. We have also shown that it is not transmitted after 8-h acquisition feed. The same occurs with Brevicoryne brassicae. Therefore, the aphid transmission factor (ATF) is involved in both means of transmission and in both aphid species. M. persicae can acquire CaMV Cabb-S strain in less than 20 s. M. persicae is a more efficient vector during a short feed than during a long feed, contrary to B. brassicae which transmits better during a long feed.
Subject(s)
Aphids/microbiology , Mosaic Viruses/genetics , Plants/microbiology , Animals , Chromosome Deletion , Epidemiology , Open Reading FramesABSTRACT
A panel of monoclonal antibodies (mAb) produced against peanut clump virus (PCV) was used to characterize five serotypes of the virus. Four different formats of enzyme-linked immunosorbent assays (ELISA) were compared to establish the most suitable one for diagnosis of infected plants and for serotype differentiation. Since most mAb retained their activity when used for coating microtitre plates, a dual mAb-type assay was found to be most suitable. The same mAb could be used in ELISA as coating and as biotinylated antibody. Because of the ability of mAb to recognize subtle conformational alterations in the viral antigen, it is important to carefully select the ELISA format used for comparing different viral isolates.
Subject(s)
Antibodies, Monoclonal/immunology , Antibodies, Viral/immunology , Arachis/microbiology , Capsid/immunology , Plant Viruses/immunology , Animals , Biotin , Capsid/isolation & purification , Enzyme-Linked Immunosorbent Assay , Epitopes/immunology , Hybridomas , Mice , Mice, Inbred BALB C , Plant Viruses/classification , Plant Viruses/isolation & purification , SerotypingABSTRACT
We have used electron microscopy of thin sections and experiments on isolated viroplasms to compare the properties of four strains of cauliflower mosaic virus (CaMV), three of which were partially or completely deleted in open reading frame (ORF) II. Our results confirm that this gene is required for aphid transmissibility and show that the product of ORF II influences the firmness with which virions are held within the viroplasm. Analysis of the proteins in the viroplasms showed that a mutant with a partial deletion in ORF II produced a protein smaller than the normal ORF product. This smaller protein was non-functional with respect both to aphid transmissibility and properties of the viroplasms.
