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1.
Acta Stomatol Croat ; 58(1): 18-29, 2024 Mar.
Article in English | MEDLINE | ID: mdl-38562221

ABSTRACT

Objectives: To compare three different orthodontic adhesives (Transbond XT Light Cure Adhesive, Heliosit Orthodontic, Fuji Ortho LC) bonded to two types of orthodontic brackets: ceramic brackets (Fascination Roth 0.22) and metallic brackets (Topic Roth 0.22, Dentaurum). Materials and methods: The study was performed on 18 human teeth (6 for each adhesive). The prepared teeth were divided into three groups according to the examination time. Subsequently, they were observed after 1, 2 and 3 weeks following bonding. After the experimental procedure, the teeth samples were cut in half along the longitudinal axis in the vestibulo-oral direction, fixed with conductive carbon cement, placed in a high-vacuum evaporator and then coated with carbon. One half of each sample was observed under a Field-emission gun scanning electron microscope (FEG-SEM Hitachi SU 8030, Japan), while on the second half of the samples qualitative (X-ray line-scans) and semi-quantitative point X-ray energy dispersive analyses (EDX) were performed with Thermo Noran (USA) NSS System 7, equipped with Ultra Dry detector (30 mm2 window). Results: Transbond XT had an ideal bond with the enamel and the bracket base, with rare presence of microgaps and cracks in the enamel. Heliosit Orthodontic demonstrated a better bond relationship with the bracket base than the enamel, whereas in the latter the presence of microgaps in the bond was observed. The microphotographs of Fuji Ortho LC demonstrated many cracks inside the adhesive, and some of them continued to move forward into the enamel surface. Therefore, an impression of a very solid bond relationship with the enamel exists, with cracks being present in the enamel surface and never at the enamel-adhesive interface. Microgaps also appeared at the bracket-adhesive interface. Conclusion: Transbond XT is a highly filled composite resin and is an ideal orthodontic adhesive in each aspect examined, with an ideal enamel-adhesive and bracket-adhesive interface. Heliosit Orthodontic provides better bracket-adhesive interface compared to the enamel. Fuji Ortho LC as a solid resin-modified GIC provides a better enamel-adhesive interface, compared to the bracket base.

2.
Lasers Med Sci ; 38(1): 145, 2023 Jun 22.
Article in English | MEDLINE | ID: mdl-37347307

ABSTRACT

The purpose of the study was to determine the influence of preparation techniques on marginal adaptation and sealing of Biodentine™ and TotalFill® RRM bioceramic retrograde fillings. Fifty-two single-root teeth extracted for periodontal reasons were used. Root canals were instrumented using Reciproc Blue #25 and obturated using a single cone technique with an AH Plus® root canal sealer. Retrograde cavities were prepared with Piezomed device (Piezo), Er:YAG laser in short-pulse(SP) and quantum square pulse(QSP) modes and filled with Biodentine™ (BD) or TotalFill® RRM (TF). There were 6 groups (n=8): (1) Piezo BD, (2) Piezo TF, (3) SP BD, (4) SP TF, (5) QSP BD, and (6) QSP TF, and positive and negative controls (n=2). Micro-CT analysis was performed on two samples from each group. Percentage volumes of internal and external voids in apical 1.5 mm were determined. Rhodamine B dye leakage was done on six samples. The samples were cut longitudinally and examined under a stereomicroscope. Digital recordings were analyzed in ImageJ software. The deepest penetration of color in mm was recorded. The data were statistically analyzed using ANOVA and Duncan's test at the level of significance α=0.05. TotalFill® RRM performed significantly better than Biodentine™ in terms of sealing (p<0.05) and marginal adaptation, as evaluated by micro-CT. Sealing was significantly better in SP compared to QSP mode preparations (p<0.05). Differences between Piezomed and laser modes were not significantly different (p>0.05). Sealing was statistically significantly better with TotalFill® RRM compared to Biodentine™ and in Er:YAG SP preparations compared to Er:YAG QSP.


Subject(s)
Dental Leakage , Lasers, Solid-State , Root Canal Filling Materials , Humans , Lasers, Solid-State/therapeutic use , Ultrasonics , X-Ray Microtomography , Research Design , Heart Rate , Root Canal Preparation , Dental Pulp Cavity
3.
Eur J Dent ; 17(3): 902-909, 2023 Jul.
Article in English | MEDLINE | ID: mdl-36513339

ABSTRACT

OBJECTIVE: Atraumatic restorative treatment (ART) may be beneficial for disadvantaged populations with no or limited access to dental services. This study aimed to evaluate the survival rate of single-surface ART restorations in primary posterior teeth in children with high caries risk. MATERIALS AND METHODS: This study was conducted in six rural areas of the Republic of Kosovo, and 100 children aged 3 to 8 years participated in the study. Information was obtained from each parent/guardian regarding their children, such as sociodemographic characteristics, general health, dental history, dietary habits, oral hygiene, and fluoride exposure. The reduced Cariogram was used to estimate the risk of caries in the participants based on the seven factors specified in the program, and all the provided information were collected and entered in a computer program of the Cariogram. A pediatric dentist, accompanied by two assistants, performed 100 ART restorations in school settings using high-viscosity glass-ionomer cement (Fuji IX) following the nine steps of the ART procedure. The restorations were evaluated at 3, 6, 9, and 12 months using the ART restoration criteria. STATISTICAL ANALYSIS: Percentages, mean value, standard deviation, mean interquartile range, and difference test between arithmetic mean values were used to analyze the research results. RESULTS: Review of the average of reduced Cariogram showed that the majority of children, 72%, were at high risk of developing caries, with only 28% having a good chance of avoiding caries in the future. A total of 77% of the children in the study had never visited dentists before due to poor economic conditions and the lack of dentists in the area. The success rates of ART restorations performed in single-surface cavities in primary teeth were very encouraging, with more than 97% success rates after a 1-year follow-up period. CONCLUSION: Our results demonstrate that ART is efficient, affordable, and practical for the treatment of single-surface cavities in primary posterior teeth. Owing to its low price and atraumatic nature, ART can potentially help disadvantaged children in Kosovo access dental care.

4.
Minerva Dent Oral Sci ; 71(1): 39-47, 2022 Feb.
Article in English | MEDLINE | ID: mdl-34549574

ABSTRACT

Since the pandemic of the novel coronavirus - SARS-CoV-2 - has been declared and strict social measures have been introduced in the middle of March, including curfew, the dentistry had to adjust and find ways to better adapt itself to the new situation. This article provides a brief overview of the situation with COVID-19 in our country since the beginning of the pandemic, gives a reminder of what COVID-19 is, and which its modes of transmission are, and considers the ways of protection in dental offices considering that dentistry is one of the riskiest activities given the patient-dentist proximity and the use of specific high-speed handpieces. This review also provides an overview of the dental preventive measures taken worldwide, including in the field of Pediatric Dentistry, as well as an overview of dental patients' management, and gives possible best ways to care for healthy, sick or patients suspected for COVID-19. Finally, an extensive presentation of the way of work at the University Dental Clinical Center and the Faculty of Dental Medicine in Skopje, North Macedonia, over the last year is given, including protocols for admission of patients.


Subject(s)
COVID-19 , COVID-19/epidemiology , Child , Dentistry , Disease Outbreaks , Humans , Republic of North Macedonia/epidemiology , SARS-CoV-2
5.
Molecules ; 26(17)2021 Aug 27.
Article in English | MEDLINE | ID: mdl-34500635

ABSTRACT

A new category of commercial bulk fill composite resins (CRs) enables the placement of 4-mm-thick layers as an alternative to the traditional time-consuming incremental technique. The purpose of the present study was to compare the efficiency of the polymerization, adaptation and porosity of two high-viscosity 'sculptable' bulk fill CRs (Filtek™ Bulk Fill (3M™ ESPE, St. Paul, MN, USA) and Tetric EvoCeram® Bulk Fill (Ivoclar Vivadent AG, Schwan, Liechtenstein)) and two low-viscosity 'flowable' bulk fill CRs (SureFil® SDR™ flow (Dentsply Sirona, Charlotte, NC, USA) and Tetric EvoFlow® Bulk Fill (Ivoclar Vivadent AG, Schaan, Liechtenstein)). Cylindrical samples of the bulk fill CRs (4 mm height × 10 mm diameter) were analyzed by Fourier-transform infrared spectroscopy (FTIR) and atomic force microscopy (AFM). Additionally, occlusal cavities were prepared in twelve extracted human molars and restored with the bulk fill CRs (n = 3 for each CR). The adaptation and porosity of the bulk fill CRs were evaluated by X-ray microcomputed tomography (µCT) with a 3D morphometric analysis, and the adaptation was also analyzed by scanning electron microscopy (SEM) on longitudinal vestibulo-oral sections of the restored teeth. The AFM analysis demonstrated that the surface roughness of the SureFil® SDR™ flow was higher than that of the Tetric EvoFlow® Bulk Fill and that the surface roughness of Filtek™ Bulk Fill was higher than that of Tetric EvoCeram® Bulk Fill. µCT and SEM confirmed that the flowable bulk fill CRs had excellent adaptation to the cavity walls. The 3D morphometric analysis showed the highest and lowest degrees of porosity in Filtek™ Bulk Fill and Tetric EvoFlow® Bulk Fill, respectively. In general, the flowable bulk fill CRs exhibited better adaptation, a higher efficiency of polymerization and lower porosity than the sculptable materials.

6.
Materials (Basel) ; 14(11)2021 Jun 03.
Article in English | MEDLINE | ID: mdl-34205094

ABSTRACT

The bone healing process following osteotomy may vary according to the type of surgical instrumentation. The aim of the present in vivo study was to determine thermal changes of the bone tissue following osteotomies performed by Er:YAG laser ablation in contact and non-contact modes, piezoelectric surgery, and surgical drill using an infrared thermographic camera. For each measurement, the temperature before the osteotomy-baseline (Tbase) and the maximal temperature measured during osteotomy (Tmax) were determined. Mean temperature (ΔT) values were calculated for each osteotomy technique. The significance of the difference of the registered temperature between groups was assessed by the ANOVA test for repeated measures. Mean baseline temperature (Tbase) was 27.9 ± 0.3 °C for contact Er:YAG laser, 29.9 ± 0.3 °C for non-contact Er:YAG laser, 29.4 ± 0.3 °C for piezosurgery, and 28.3 ± 0.3 °C for surgical drill. Mean maximum temperature (Tmax) was 29.9 ± 0.5 °C (ΔT = 1.9 ± 0.3 °C) for contact Er:YAG laser, 79.1 ± 4.6 °C (ΔT = 49.1 ± 4.4 °C) for non-contact Er:YAG laser, 29.1 ± 0.2 °C (ΔT = -0.2 ± 0.3 °C) for piezosurgery, and 27.3 ± 0.4 °C (ΔT = -0.9 ± 0.4 °C) for surgical drill. Statistically significant temperature changes were observed for the non-contact laser. The results of the study showed beneficial effects of the osteotomy performed by the Er:YAG laser used in the contact mode of working as well as for piezosurgery, reducing the potential overheating of the bone tissue as determined by means of infrared thermography.

7.
Materials (Basel) ; 13(2)2020 Jan 08.
Article in English | MEDLINE | ID: mdl-31936253

ABSTRACT

The aim of the study was to evaluate the effects of incorporation of Al2O3, ZrO2 and TiO2 nanoparticles into glass-ionomer cements (GICs). Two different GICs were used in the study. Four groups were prepared for each material: the control group (without nanoparticles) and three groups modified by the incorporation of nanoparticles at 2, 5 or 10 wt %, respectively. Cements were mixed and placed in moulds (4 mm × 6 mm); after setting, the samples were stored in saline (one day and one week). Compressive strengths were measured and the morphology of the fractured surfaces was analyzed by scanning electron microscopy. The elements released into the storage solutions were determined by Inductively coupled plasma-optical emission spectrometry (ICP-OES). Addition of nanoparticles was found to alter the appearance of cements as examined by scanning electron microscopy. Compressive strength increased with the addition of ZrO2 and especially TiO2 nanoparticles, whereas the addition of Al2O3 nanoparticles generally weakened the cements. The ion release profile of the modified cements was the same in all cases. The addition of Al2O3, ZrO2 and TiO2 nanoparticles into GICs is beneficial, since it leads to reduction of the microscopic voids in the set cement. Of these, the use of ZrO2 and TiO2 nanoparticles also led to increased compressive strength. Nanoparticles did not release detectable levels of ions (Al, Zr or Ti), which makes them suitable for clinical use.

8.
Eur J Dent ; 13(2): 243-247, 2019 May.
Article in English | MEDLINE | ID: mdl-31480090

ABSTRACT

OBJECTIVE: The aim of this study is to determine the effect of the addition of benzalkonium chloride and cetylpyridinium chloride in three commercial root canal sealers. MATERIALS AND METHODS: Three different root canal sealers were used: EndoRez, N2, and Apexit Plus. The samples were prepared by mixing the components according to the manufacturers' guidelines and adding 2% in weight of the antimicrobials to the newly mixed cement. The paste was placed in molds and stored in an incubator (37°C, 24 h). The samples were then stored in 5-mL distilled water. Samples without antimicrobials served as a control. All samples were tested at 3 time intervals: 1 day, 1 week, and 1 month following their storage in distilled water. The impact of the antimicrobials on the solubility of the sealers, the release of chloride ions (Cl-), and the pH value were examined. STATISTICAL ANALYSIS: Analysis was done using one-way analysis of variance and the post hoc Tukey's honestly significant difference test. RESULTS: Chloride ions are present in storage media with EndoRez, N2, and Apexit Plus samples (without antimicrobials) following all tested storage intervals. The addition of the antimicrobials increased the release of chloride ions. Endodontic cements without addition of antimicrobials show an increase in weight after 1 month. The highest pH value is measured in Apexit Plus samples. The solutions in which N2 samples (with and without addition of antimicrobials) were stored did not have a significant change in their pH, while in the EndoRez solutions, a significant decrease in the pH value after the first week was measured. CONCLUSIONS: The addition of antimicrobials might lead to improved characteristics of the root canal sealers.

9.
Photobiomodul Photomed Laser Surg ; 37(6): 362-368, 2019 Jun.
Article in English | MEDLINE | ID: mdl-31081716

ABSTRACT

Objective: The aim of this study was to compare the efficacy of Er:YAG and Er,Cr:YSGG laser in the treatment of oral leukoplakia refractory to conventional retinoid therapy. Materials and methods: The study sample consisted of 54 patients (16 men and 38 women) who were histopathologically diagnosed with oral leukoplakia that was refractory to conventional retinoid therapy. Patients were randomly allocated into two groups according to the type of the laser used for treatment of oral leukoplakia: Group 1. Er:YAG laser; Group 2. Er,Cr:YSGG laser. Patients were recalled at 6 months and 1 year after treatment to evaluate possible recurrence and assess the patients' postoperative quality of life. Results: After initial ablation, the degree of residual lesion was significantly greater in the Er:YAG laser group (74.1%), compared with the Er,Cr:YSGG group (18.5%) (p = 0.0001). Six months and 1 year after the second ablation, there was no lesion recurrence in either laser group. Fourteen days after the initial ablation, the visual analog scale (VAS) pain rating and the total oral health impact profile score fell significantly in both groups (p < 0.0001). However, in the Er,Cr:YSGG laser group, the average value of the VAS rating was significantly lower than in the Er:YAG laser group (p = 0.039). Conclusions: The Er:YAG and Er,Cr:YSGG lasers showed similar efficacy in the treatment of oral leukoplakia and resulted in full postoperative recovery without recurrence after 1 year of follow-up.


Subject(s)
Laser Therapy/methods , Lasers, Solid-State/therapeutic use , Leukoplakia, Oral/radiotherapy , Female , Humans , Leukoplakia, Oral/drug therapy , Male , Middle Aged , Pain Measurement , Quality of Life , Retinoids/therapeutic use
10.
Int J Oral Maxillofac Implants ; 33(4): 831-837, 2018.
Article in English | MEDLINE | ID: mdl-30024999

ABSTRACT

PURPOSE: The aim of this study was to evaluate the efficacy of photodynamic therapy (PDT) and light-activated disinfection (LAD) against a 3-day-old bacterial suspension prepared from three different bacterial species present on titanium dental implants, and to analyze the possible alterations of the implant surfaces as a result of the PDT and LAD. MATERIALS AND METHODS: The study was conducted on 72 titanium dental implants contaminated with a bacterial suspension prepared from three bacterial species: Prevotella intermedia, Aggregatibacter actinomycetemcomitans, and Porphyromonas gingivalis. The contaminated implants were incubated under anaerobic conditions for 72 hours and then were randomly divided into four experimental groups and two control groups (n = 12 each), according to the following treatment protocols: group 1 (PDT1): PDT (660 nm, 100 mW, 60 seconds) with toluidine blue; group 2 (PDT2): PDT (660 nm, 100 mW, 60 seconds) with phenothiazine chloride dye; group 3 (LAD): light-emitting diode (LED) with toluidine blue; group 4 (toluidine blue): treatment with only toluidine blue for 60 seconds. In the positive control group, the implants were treated with a 0.2% chlorhexidine-based solution for 60 seconds, and in the negative control group, no treatment was used. RESULTS: The highest bacterial reduction was recorded in the PDT1 (98.3%) and PDT2 (97.8%) groups. The results of this study showed that there was a statistically significant reduction of bacteria in the PDT1 and PDT2 groups compared with the negative control group (P < .05), individually for each bacterial species as well as for all three species together. LAD was less effective than PDT1 and PDT2, and did not show a statistically significant difference compared with the negative control or any other treatment group. Toluidine blue was the least effective treatment in terms of both the total bacterial count and the individual count for each bacterial species. CONCLUSION: Both PDT1 and PDT2 protocols showed a high efficacy against a 3-day-old bacterial biofilm on dental implants and were more effective compared with LAD.


Subject(s)
Bacteria/drug effects , Biofilms/drug effects , Dental Implants/microbiology , Disinfection/methods , Photochemotherapy/methods , Photosensitizing Agents/pharmacology , Titanium , Aggregatibacter actinomycetemcomitans/drug effects , Aggregatibacter actinomycetemcomitans/physiology , Anti-Bacterial Agents/pharmacology , Anti-Infective Agents , Bacterial Load , Chlorhexidine/pharmacology , Humans , Lasers, Semiconductor , Light , Porphyromonas gingivalis/drug effects , Porphyromonas gingivalis/physiology , Prevotella intermedia/drug effects , Prevotella intermedia/physiology , Tolonium Chloride/pharmacology
11.
Life Sci ; 197: 10-18, 2018 Mar 15.
Article in English | MEDLINE | ID: mdl-29391192

ABSTRACT

AIM: To test the antioxidant properties of the newly synthesized (2E,6E)-2,6-bis(2-bromobenzylidene)cyclohexanone (B2BrBC) in parallel with C66 in rats with cardiac hypertrophy. MATERIALS AND METHODS: The protective effects of both C66 and B2BrBC against oxidative stress in rats with cardiac hypertrophy, was studied by evaluating the activity of antioxidant enzymes, the relationship between the ratio of the activities of the antioxidant enzymes R = SOD/(GPx + CAT) and levels of thiols and lipid peroxidation in the heart. In order to gain better understanding of the antioxidant properties of the studied compounds, computational methods were utilized. The properties of selected structurally related derivatives were obtained on optimized geometries for ground states, using semi-empirical PM3 quantum mechanical calculations. KEY FINDINGS: The ratio R shows disequilibrium in rats with induced hypertrophy (p < 0.001). Coextending changes were detected in total and free sulfhydryl group content (p = 0.011 for t-SH and p = 0.008, for free SH, respectively). The results with the B2BrBC, indicated strong thiol prevention reflected in the levels of both t-SH and f-SH. Taking into account the HOMO energies of B2BrBC (-9.398 eV) and C66 (-9.667), it can be concluded that B2BrBC has lower HOMO energy, which makes it a better electron donor and a better antioxidant. SIGNIFICANCE: The obtained results indicated that the antioxidant ability of B2BrBC is positively associated with the catalytic SOD and GPx activities expressed through preserved t-SH levels. It seems plausible that for a compound to exhibit antioxidant activity, as most of the 2,6-bis(benzylidene)cyclohexanones do, they should be good electron donors. IMPACT STATEMENT: Understanding the relationship between cardiac hypertrophy induced oxidative injuries and supporters of endogenous reparatory machinery will help in establishing the beneficial role of adequate antioxidant supplementation. In this study reliable data on the preventive effects of newly synthesized symmetric monocarbonyl curcumin analogue B2BrBC and its role in the prevention of oxidative injuries on three levels (enzymatic, protein and lipid), in the heart hypertrophic onset, were obtained.


Subject(s)
Antioxidants , Cardiomegaly , Curcumin , Isoproterenol/adverse effects , Lipid Peroxidation/drug effects , Oxidative Stress/drug effects , Animals , Antioxidants/chemistry , Antioxidants/pharmacology , Cardiomegaly/chemically induced , Cardiomegaly/drug therapy , Cardiomegaly/metabolism , Curcumin/analogs & derivatives , Curcumin/chemistry , Curcumin/pharmacology , Isoproterenol/pharmacology , Male , Myocardium/metabolism , Rats , Rats, Wistar
12.
Biomed Res Int ; 2017: 2129807, 2017.
Article in English | MEDLINE | ID: mdl-28620615

ABSTRACT

Root canal sealers with antimicrobial activity are highly beneficial; therefore, their antimicrobial properties could be improved by incorporation of antimicrobial agents. In the present study, the release of the quaternary ammonium compounds from endodontic sealers admixed with either benzalkonium chloride (BC) or cetylpyridinium chloride (CPC) at loadings of 2% wt was monitored. The effect of these additives on the compressive strengths and their release from the sealers was determined after 1 and 4 weeks. All of the materials studied were found to be capable of releasing antimicrobial additive in useful quantities. The release of CPC occurred to a statistically significant greater extent than BC for all materials. The addition of both BC and CPC generally decreased the compressive strength of all the endodontic sealers, with the exception of CPC in AH Plus, where the compressive strength was significantly increased. This suggests that, for these endodontic sealers, the antimicrobial additives alter the setting chemistry. AH Plus is an epoxy-based material cured with an amine, and in this case the increase in compressive strength with CPC is attributed to an enhanced cure reaction with this system. In all other cases, the additive inhibited the cure reaction to a greater or lesser extent.


Subject(s)
Anti-Infective Agents/pharmacology , Benzalkonium Compounds/chemistry , Cetylpyridinium/chemistry , Root Canal Filling Materials/chemistry , Anti-Infective Agents/chemistry , Benzalkonium Compounds/pharmacology , Cetylpyridinium/pharmacology , Enterococcus faecalis/drug effects , Epoxy Resins/chemistry , Epoxy Resins/therapeutic use , Humans , Materials Testing , Quaternary Ammonium Compounds/chemistry , Quaternary Ammonium Compounds/therapeutic use , Root Canal Filling Materials/pharmacology , Zinc Oxide-Eugenol Cement/chemistry , Zinc Oxide-Eugenol Cement/therapeutic use
13.
Oman Med J ; 32(3): 195-200, 2017 May.
Article in English | MEDLINE | ID: mdl-28584599

ABSTRACT

OBJECTIVES: Iron overload can cause or contribute to the pathogenesis of type 2 diabetes mellitus (T2DM), but how the major parameters of iron metabolism change in different settings of diabetes are still unclear. The aim of this study was to determine the relationship between iron, ferritin, and hepcidin levels in diabetic patients and the effect of insulin treatment. METHODS: The study included 80 subjects, 60 with T2DM and 20 without (control group). Serum hepcidin, insulin, ferritin, and iron levels were determined as well as other clinical parameters. The associations between these parameters were analyzed between both groups. RESULTS: Hepcidin levels expressed as mean± standard deviation between groups showed no significant changes (14.4±6.7 ng/mL for the control group, and 18.4±7.9 ng/mL for patients with diabetes, p = 0.069). Parameters of iron metabolism showed modest correlation with the parameters of glucose metabolism. However, the correlation between ferritin and insulin in both groups was statistically significant (p = 0.032; ρ = 0.480 vs. p = 0.011; ρ = 0.328). CONCLUSIONS: Our study showed that hepcidin levels in patients with T2DM on insulin therapy do not change, which might be a result of treatment with insulin. In this context, insulin treatment can be used as a novel method for correction of hepcidin levels. By correcting hepcidin levels, we can prevent cellular iron overload and reduce the risk of diabetes.

15.
Microsc Microanal ; 22(6): 1170-1178, 2016 12.
Article in English | MEDLINE | ID: mdl-27998364

ABSTRACT

The aim of the present study was to compare dynamics of the bone healing process after different types of osteotomies. In total, 24 Wistar rats were subjected to different types of osteotomy performed with standard steel bur, piezosurgery, contact, and non-contact Erbium:yttrium-aluminum-garnet (Er:YAG) laser ablation. The animals were randomly divided into four groups, to be euthanized immediately after the procedure, or at 1, 2, or 3 weeks after surgery. The obtained bone samples were analyzed by scanning electron microscopy (SEM). Immediately after surgery, there were significant differences in the appearance of the bone defects, with presence of bone fragments and debris after standard steel bur preparation, compared with the clean smooth walls and relatively sharp edges in all other groups. The initial bone formation in defects prepared by piezosurgery was observed to be the most rapid. After 3 weeks, all bone defects were completely restored; although, differences in the healing pattern were noted, with a modest initial delay in healing after laser preparation. The first stage of the bone healing process was delayed when contact and non-contact Er:YAG laser modes were used and accelerated by piezosurgery; however, the results after 3 weeks demonstrated similar restitution of defects in all tested groups.


Subject(s)
Bone and Bones/ultrastructure , Microscopy, Electron, Scanning , Osteotomy , Wound Healing , Animals , Bone and Bones/radiation effects , Bone and Bones/surgery , Lasers, Solid-State , Osteogenesis , Random Allocation , Rats , Rats, Wistar
16.
J Microsc ; 262(3): 232-44, 2016 06.
Article in English | MEDLINE | ID: mdl-27197087

ABSTRACT

The purpose of this study was to compare the effects of professional tooth whitening agents containing highly concentrated hydrogen peroxide (with and without laser activation), on the enamel surface; and the potential of four different toothpastes to remineralize any alterations. The study was performed on 50 human molars, divided in two groups: treated with Opalescence(®) Boost and Mirawhite(®) Laser Bleaching. Furthermore, each group was divided into five subgroups, a control one and 4 subgroups remineralized with: Mirasensitive(®) hap+, Mirawhite(®) Gelleѐ, GC Tooth Mousse™ and Mirafluor(®) C. The samples were analysed by SEM/3D-SEM-micrographs, SEM/EDX-qualitative analysis and SEM/EDX-semiquantitative analysis. The microphotographs show that both types of bleaching cause alterations: emphasized perikymata, erosions, loss of interprizmatic substance; the laser treatment is more aggressive and loss of integrity of the enamel is determined by shearing off the enamel rods. In all samples undergoing remineralization deposits were observed, those of toothpastes based on calcium phosphate technologies seem to merge with each other and cover almost the entire surface of the enamel. Loss of integrity and minerals were detected only in the line-scans of the sample remineralized with GC Tooth Mousse™. The semiquantitative EDX analysis of individual elements in the surface layer of the enamel indicates that during tooth-bleaching with HP statistically significant loss of Na and Mg occurs, whereas the bleaching in combination with a laser leads to statistically significant loss of Ca and P. The results undoubtedly confirm that teeth whitening procedures lead to enamel alterations. In this context, it must be noted that laser bleaching is more aggressive for dental substances. However, these changes are reversible and can be repaired by application of remineralization toothpastes.


Subject(s)
Dental Enamel/chemistry , Dental Enamel/drug effects , Hydrogen Peroxide/adverse effects , Molar/drug effects , Tooth Bleaching Agents/adverse effects , Tooth Bleaching/adverse effects , Tooth Remineralization , Calcium/analysis , Calcium/deficiency , Humans , Lasers , Magnesium/analysis , Molar/chemistry , Phosphorus/analysis , Phosphorus/deficiency , Sodium/analysis , Sodium/deficiency , Tooth Remineralization/methods , Toothpastes
17.
Microsc Microanal ; 21(2): 392-406, 2015 Apr.
Article in English | MEDLINE | ID: mdl-25691120

ABSTRACT

Conventional glass-ionomer cements (GICs) are popular restorative materials, but their use is limited by their relatively low mechanical strength. This paper reports an attempt to improve these materials by incorporation of 10 wt% of three different types of nanoparticles, aluminum oxide, zirconium oxide, and titanium dioxide, into two commercial GICs (ChemFil® Rock and EQUIA™ Fil). The results indicate that the nanoparticles readily dispersed into the cement matrix by hand mixing and reduced the porosity of set cements by filling the empty spaces between the glass particles. Both cements showed no significant difference in compressive strength with added alumina, and ChemFil® Rock also showed no significant difference with zirconia. By contrast, ChemFil® Rock showed significantly higher compressive strength with added titania, and EQUIA™ Fil showed significantly higher compressive strength with both zirconia and titania. Fewer air voids were observed in all nanoparticle-containing cements and this, in turn, reduced the development of cracks within the matrix of the cements. These changes in microstructure provide a likely reason for the observed increases in compressive strength, and overall the addition of nanoparticles appears to be a promising strategy for improving the physical properties of GICs.


Subject(s)
Acrylic Resins/chemistry , Dental Cements/chemistry , Mechanical Phenomena , Metal Nanoparticles/analysis , Silicon Dioxide/chemistry , Materials Testing
18.
Microsc Microanal ; 19(6): 1450-7, 2013 Dec.
Article in English | MEDLINE | ID: mdl-24148964

ABSTRACT

Three different bioactive materials suitable as dentine substitutes in tooth repair have been studied: glass-ionomer cement, particulate bioglass, and calcium-silicate cement. On 15 permanent human molars, Class V cavities were prepared and the bottom of each cavity was de-mineralized by an artificial caries gel. After the de-mineralization, the teeth were restored with: (1) Bioglass®45S5 and ChemFil® Superior; (2) Biodentine™ and ChemFil® Superior; and (3) ChemFil® Superior for a complete repair. The teeth were stored for 6 weeks in artificial saliva, then cut in half along the longitudinal axis: the first half was imaged in a scanning electron microscope (SEM) and the other half was embedded in resin and analyzed by SEM using energy-dispersive X-ray analysis. The glass-ionomer and the bioglass underwent ion exchange with the surrounding tooth tissue, confirming their bioactivity. However, the particle size of the bioglass meant that cavity adaptation was poor. It is concluded that smaller particle size bioglasses may give more acceptable results. In contrast, both the glass-ionomer and the calcium-silicate cements performed well as dentine substitutes. The glass-ionomer showed ion exchange properties, whereas the calcium silicate gave an excellent seal resulting from its micromechanical attachment.


Subject(s)
Dental Materials , Dental Restoration, Permanent/methods , Dental Caries/therapy , Humans , In Vitro Techniques , Microscopy, Electron, Scanning
19.
Microsc Microanal ; 19(3): 587-95, 2013 Jun.
Article in English | MEDLINE | ID: mdl-23659606

ABSTRACT

Remineralization of hard dental tissues is thought to be a tool that could close the gap between prevention and surgical procedures in clinical dentistry. The purpose of this study was to examine the remineralizing potential of different toothpaste formulations: toothpastes containing bioactive glass, hydroxyapatite, or strontium acetate with fluoride, when applied to demineralized enamel. Results obtained by scanning electron microscopy (SEM) and SEM/energy dispersive X-ray analyses proved that the hydroxyapatite and bioactive glass-containing toothpastes were highly efficient in promoting enamel remineralization by formation of deposits and a protective layer on the surface of the demineralized enamel, whereas the toothpaste containing 8% strontium acetate and 1040 ppm fluoride as NaF had little, if any, remineralization potential. In conclusion, the treatment of demineralized teeth with toothpastes containing hydroxyapatite or bioactive glass resulted in repair of the damaged tissue.


Subject(s)
Dental Enamel/chemistry , Minerals/analysis , Tooth Demineralization/therapy , Toothpastes/therapeutic use , Humans , Imaging, Three-Dimensional , In Vitro Techniques , Microscopy, Electron, Scanning , Spectrometry, X-Ray Emission
20.
J Mater Sci Mater Med ; 23(7): 1811-21, 2012 Jul.
Article in English | MEDLINE | ID: mdl-22532098

ABSTRACT

This study was carried out in order to determine the extent to which ions released from fluoride-containing dental restoratives migrated through the enamel and dentine of extracted teeth. A total of 40 permanent human 3rd molars were used. They were extracted for orthodontic reasons, and employed within 1 month of extraction. A cervical (Class V) cavity was prepared in each tooth, then filled with one of: a conventional glass-ionomer, a resin-modified glass-ionomer, a polyacid-modified composite resin ("compomer") or a fluoride-releasing resin composite. Ten samples were prepared per material. After 1 month, five specimens per material were prepared and examined under SEM/EDX. Concentrations of sodium, aluminium, strontium, fluorine, magnesium, silicon, phosphorus and calcium were determined within the tooth. After 18 months, the remaining five specimens for each material were prepared and studied in the same way. The greatest extent of ion migration into the tooth was found with the conventional glass-ionomer and least migration was found for the fluoride-releasing composite, which showed no evidence of fluoride migration at all. Levels of migrating ions were generally higher in the 18 month specimens than in the 1 month specimens, and also higher in the dentine than in the enamel. Ions released by restorative dental materials have been shown conclusively for the first time to be capable of migrating into the enamel and dentine surrounding the restoration. The conventional glass-ionomer showed the highest level of ion migration whereas the fluoridated composite resin showed little if any ion migration. This suggests that the conventional glass-ionomer has the greatest caries inhibiting effects of all the materials tested, and the fluoridated composite the least.


Subject(s)
Dental Materials , Fluorides/administration & dosage , Humans , Ions , Microscopy, Electron, Scanning
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