Survival and transfer efficacy of mixed strain Salmonella enterica ser. Typhimurium from beef burgers to abiotic surfaces and determination of individual strain contribution.

The aim of the study was to evaluate the survival and transfer efficacy of 3 Salmonella Typhimurium strains from beef burgers to abiotic surfaces and determine the individual strain distribution. S. Typhimurium population on beef burgers during incubation remained constant at initial levels of contamination approximately 3 and 5 log CFU/g. Additionally, the survival of pathogens on soiled HDPE surfaces was significant during incubation at both initial inocula, while ca 1.5 log CFU/cm2 reduction was observed at 168h. The log transformed transfer rate (log10Tr) was -1.86±0.23 and -1.75±0.40 for high and low inoculum. The level of initial contamination did not have any statistical important impact on bacterial transfer (P>0.05). In addition, the results regarding the strain contribution revealed rather random individual proportion of each strain, recovered from HDPE, SS surfaces and beef burgers. However, the dominance of each strain was strongly dependent on surface at low inoculum and time in case of high inoculum. This observed strain variability during survival and transfer of S. Typhimurium might be of great importance in order to understand and consequently limit the possibility of cross contamination during food processing in a common household.

Transfer of foodborne pathogenic bacteria to non-inoculated beef fillets through meat mincing machine.

The aim of this study was to evaluate the transfer of pathogens population to non-inoculated beef fillets through meat mincing machine. In this regard, cocktails of mixed strain cultures of each Listeria monocytogenes, Salmonella enterica ser. Typhimurium and Escherichia coli O157:H7 were used for the inoculation of beef fillets. Three different initial inoculum sizes (3, 5, or 7 log CFU/g) were tested. The inoculated beef fillets passed through meat mincing machine and then, six non-inoculated beef fillets passed in sequence through the same mincing machine without sanitation. The population of each pathogen was measured. It was evident that, all non-inoculated beef fillets were contaminated through mincing with all pathogens, regardless the inoculum levels used. This observation can be used to cover knowledge gaps in risk assessments since indicates the potential of pathogen contamination and provides significant insights for the risk estimation related to cross-contamination, aiming thus to food safety enhancement.