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1.
Scand J Immunol ; 58(1): 81-8, 2003 Jul.
Article in English | MEDLINE | ID: mdl-12828562

ABSTRACT

Chronic relapsing experimental autoimmune encephalomyelitis (ChREAE) is an autoimmune disease of the central nervous system (CNS) induced by CNS myelin components. In the early active stage, both ChREAE and multiple sclerosis (MS) are characterized by the presence of perivascular inflammatory cuffs disseminated in the CNS. There is growing evidence that chemoattractant cytokines (chemokines) play an important role in this process. The main goal of the present study was to analyse the hypothesis that chemokine expression in the CNS during autoimmune inflammation is regulated by proinflammatory cytokines. To address this concept, we analysed temporal relations between chemokine and cytokine expression during ChREAE. Phasic upregulation of gene expression for chemokines T-cell activation gene 3 (TCA-3)/CCL1, monocyte chemoattractant protein-1 (MCP-1)/CCL2, macrophage inflammatory protein-1 alpha (MIP-1alpha)/CCL3, MIP-1beta/CCL4, regulated on activation normal T cell expressed and secreted (RANTES)/CCL5 and MIP-2/CXCL2-3 as well as cytokines tumour necrosis factor-alpha (TNF-alpha), -beta, LT-beta, interferon-gamma (IFN-gamma) and transforming growth factor-beta1 (TGF-beta1) in the CNS was observed during attacks of ChREAE. Expression of cytokines TNF-beta and LT-beta preceded, and the expression of TGF-beta1 followed chemokine upregulation. Our results suggest that chemokine expression during CNS autoimmune inflammation may be regulated by some proinflammatory cytokines.


Subject(s)
Chemokines/biosynthesis , Cytokines/biosynthesis , Encephalomyelitis, Autoimmune, Experimental/immunology , Animals , Chronic Disease , Female , Mice , Spinal Cord/immunology , Spleen/immunology , Up-Regulation
2.
Neurol Neurochir Pol ; 35(6): 1111-9, 2001.
Article in Polish | MEDLINE | ID: mdl-11987706

ABSTRACT

Gene therapy is a new therapeutic method which uses molecular biology techniques to modulate expression of genes involved in disease pathogenesis. Gene therapy has evolved rapidly in the last ten years. In that time several experimental models of metabolic and degenerative neurological disorders, brain tumours, stroke, epilepsy, motor neuron disease, multiple sclerosis and muscle dystrophy were treated with some positive results by gene therapy techniques. The first clinical trials are currently ongoing in certain metabolic diseases, brain tumours and limb girdle dystrophy. DNA vaccination is also used in clinical trials. It seems that gene therapy will become soon the alternative method of treatment of those neurological diseases where current methods of treatment are not effective.


Subject(s)
Genetic Therapy/methods , Nervous System Diseases/genetics , Nervous System Diseases/therapy , Animals , Clinical Trials as Topic , Disease Models, Animal , Gene Transfer Techniques , Humans
3.
Ann N Y Acad Sci ; 917: 135-44, 2000.
Article in English | MEDLINE | ID: mdl-11268337

ABSTRACT

Chemokines are small proinflammatory cytokines that possess the ability to stimulate migration of inflammatory cells towards the tissue site of inflammation. Previous reports showed that several chemokines may be involved in the pathogenesis of experimental autoimmune encephalomyelitis (EAE), an animal model of autoimmune central nervous system (CNS) inflammation. Inflammatory cells respond to chemotactic chemokine gradient through the chemokine receptors (ChRs). The goal of this study was to analyze expression of ChRs belonging to CXC subfamily during different stages of chronic relapsing EAE. We found significantly increased expression of CXCR2 and CXCR4 in the spinal cord during the first and second disease attacks. The kinetics of this expression in CNS and blood suggests that CXCR2 is expressed by leukocytes migrating from the blood, but CXCR4 is expressed mainly by CNS parenchymal cells. Those results support the interpretation that chemokine-chemokine receptor interactions may play an important role in the development of CNS autoimmune inflammation.


Subject(s)
Encephalomyelitis, Autoimmune, Experimental/immunology , Receptors, CXCR4/immunology , Receptors, Interleukin-8B/immunology , Animals , Chronic Disease , Female , Mice , Recurrence
5.
Neurol Neurochir Pol ; 33(4): 897-906, 1999.
Article in Polish | MEDLINE | ID: mdl-10612104

ABSTRACT

Chemokines are a family of proinflammatory cytokines which are able to stimulate directional migration of leukocytes in vitro and in vivo. Because of this feature chemokines may be potent mediators of inflammatory processes. Numerous observations indicate that chemokines may be involved in the pathogenesis of autoimmune and infectious inflammation within the central nervous system (CNS). Moreover, there are many reports showing increased expression of some chemokines in experimental models of brain mechanical injury and ischaemia. Several lines of cultured CNS cells are able to produce chemokines in vitro. All those data suggest that chemokines are important mediators of CNS pathology and that they can be a promising target for future therapy of neurological diseases.


Subject(s)
Brain/metabolism , Brain/pathology , Central Nervous System Diseases/diagnosis , Central Nervous System Diseases/metabolism , Central Nervous System Neoplasms/diagnosis , Central Nervous System Neoplasms/metabolism , Chemokines/metabolism , Humans
7.
J Neurovirol ; 5(1): 95-101, 1999 Feb.
Article in English | MEDLINE | ID: mdl-10190695

ABSTRACT

Experimental autoimmune encephalomyelitis (EAE) is an inflammatory disease of the central nervous system (CNS) considered to be an animal model for multiple sclerosis (MS). The detailed mechanism that specifies accumulation of inflammatory cells within the CNS in these conditions remains a subject of active investigation. Chemokines including IP-10, GRO-alpha, MCP-1 are produced in EAE tissues selectively by parenchymal astrocytes, but the regulatory stimuli that govern this expression remain undetermined. The unexpected occurrence of increased EAE susceptibility in Balb/c GKO mice (lacking IFN-gamma) offered an opportunity to examine the spectrum of chemokine expression during immune-mediated inflammation in the absence of a single regulatory cytokine. We found that chemokines MCP-1 and GRO-alpha were upregulated in the CNS of mice with EAE despite the GKO genotype. IP-10, which is highly expressed in the CNS of mice with an intact IFN-gamma gene and EAE, was strikingly absent. In vitro experiments confirmed that IFNgamma selectively stimulates astrocytes for IP-10 expression. These results indicate that IP-10 is dependent upon IFN-gamma for its upregulation during this model disease, and document directly that astrocyte expression of chemokines during EAE is governed by pro-inflammatory cytokines.


Subject(s)
Chemokines/metabolism , Encephalomyelitis, Autoimmune, Experimental/metabolism , Intercellular Signaling Peptides and Proteins , Animals , Astrocytes/metabolism , Cattle , Central Nervous System/metabolism , Chemokine CCL2/metabolism , Chemokine CXCL1 , Chemokine CXCL10 , Chemokines, CXC/metabolism , Chemotactic Factors/metabolism , Disease Models, Animal , Growth Substances/metabolism , Interferon-gamma , Liver/metabolism , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Knockout , RNA, Messenger/analysis , Time Factors
8.
Neuroimmunomodulation ; 5(3-4): 166-71, 1998.
Article in English | MEDLINE | ID: mdl-9730682

ABSTRACT

Experimental autoimmune encephalomyelitis (EAE) is an investigator-initiated disorder that serves as an animal model for the common human demyelinating disease multiple sclerosis. Both diseases are typified by disseminated perivascular and submeningeal cuffs in the central nervous system (CNS). It was shown recently that chemokines are integral to the pathogenesis of EAE. In the present study we analyzed the gene expression of three chemokines, RANTES, MIP-1alpha and GRO-alpha, at the onset of acute EAE, and correlated that expression with the intensity of inflammatory changes in the CNS. We showed that all three chemokines are upregulated simultaneously with symptom onset of acute EAE, and that chemokine expression correlates with the intensity of inflammation in the CNS. This consistent relationship supports the hypothesis that chemokines are relevant to leukocyte accumulation in CNS parenchyma.


Subject(s)
Chemokine CCL5/genetics , Chemokines, CXC , Chemotactic Factors/genetics , Encephalomyelitis, Autoimmune, Experimental/immunology , Growth Substances/genetics , Intercellular Signaling Peptides and Proteins , Macrophage Inflammatory Proteins/genetics , Acute Disease , Animals , Brain Chemistry/immunology , Chemokine CCL3 , Chemokine CCL4 , Chemokine CCL5/immunology , Chemokine CXCL1 , Chemotactic Factors/immunology , Female , Gene Expression/immunology , Growth Substances/immunology , Liver/immunology , Liver/metabolism , Macrophage Inflammatory Proteins/immunology , Mice , Mice, Inbred Strains , Monocytes/immunology , Spinal Cord/chemistry , Spinal Cord/immunology , T-Lymphocytes/immunology
9.
Acta Neuropathol ; 95(1): 98-103, 1998 Jan.
Article in English | MEDLINE | ID: mdl-9452827

ABSTRACT

Traumatic injury to the brain initiates multiple interrelated processes that involve parenchymal, vascular, and infiltrating inflammatory cells. Nitric oxide (NO) and chemokines have been implicated as regulators of the central nervous system injury response. Following a cryogenic lesion of the cerebral cortex in mice, mRNA for NO synthase (NOS)-2 was detected by reverse transcriptase polymerase chain reaction ipsilaterally 12 h after injury and persisted for 2 weeks. While mRNA was also detected contralaterally, the time course of expression was shorter (1 week). By immunohistochemistry, NOS-2 protein was initially detected ipsilaterally 12 h after injury in infiltrating inflammatory cells. Astroglial cells expressed NOS-2 from 24 to 72 h after injury. The expression of monocyte chemoattractant protein (MCP-1) mRNA peaked at 6 h on the lesion side, remained for 24 h and then declined by 48 h. On the unlesioned side, MCP-1 mRNA was expressed to a much lesser extent and had declined by 24 h. The up-regulation of MCP-1 was relatively specific as a closely related mRNA encoding IP-10 was not significantly increased. These findings implicate a role for NOS-2 and MCP-1 as potential regulators of cellular events following cryogenic cerebral trauma.


Subject(s)
Brain Injuries/enzymology , Chemokine CCL2/biosynthesis , Nitric Oxide Synthase/biosynthesis , Animals , Astrocytes/enzymology , Cats , Female , Immunohistochemistry , Mice , Mice, Inbred BALB C , Polymerase Chain Reaction
10.
Am J Pathol ; 150(2): 617-30, 1997 Feb.
Article in English | MEDLINE | ID: mdl-9033275

ABSTRACT

Chemokines are secreted peptides that exhibit selective chemoattractant properties for target leukocytes. Two subfamilies, alpha- and beta-chemokines, have been described, based on structural, genetic, and functional considerations. In acute experimental autoimmune encephalomyelitis (EAE), chemokines are up-regulated systemically and in central nervous system (CNS) tissues at disease onset. Functional significance of this expression was supported by other studies; intervention with an antichemokine antibody abrogated passive transfer of EAE, and chemokines expressed in brains of transgenic mice recruited appropriate leukocyte populations into the CNS compartment. Chemokine expression in the more relevant circumstance of chronic EAE has not been addressed. We monitored the time course and cellular sources of chemokines (monocyte chemoattractant protein-1, macrophage inflammatory protein-1 alpha, interferon-gamma-inducible protein of 10 kd, KC, and regulated on activation, normal T-cell expressed and secreted cytokine) in CNS and peripheral tissues during spontaneous relapses of chronic EAE. We found coordinate chemokine up-regulation in brain and spinal cord during clinical relapse, with expression confined to CNS tissues. Monocyte chemoattractant protein-1, interferon-gamma-inducible protein of 10 kd, and KC were synthesized by astrocytic cells, whereas macrophage inflammatory protein-1 alpha and regulated on activation, normal T-cell expressed and secreted cytokine were elaborated by infiltrating leukocytes. The results demonstrate stringent regulation of multiple chemokines in vivo during a complex organ-specific autoimmune disease. We propose that chemokine expression links T-cell antigen recognition and activation to subsequent CNS inflammatory pathology in chronic relapsing EAE.


Subject(s)
Central Nervous System/metabolism , Chemokines/biosynthesis , Encephalomyelitis, Autoimmune, Experimental/metabolism , Neurons/metabolism , Animals , Cell Line , Central Nervous System/pathology , Chemokines/genetics , Chronic Disease , Encephalomyelitis, Autoimmune, Experimental/pathology , Female , Kinetics , Liver/metabolism , Mice , Mice, Inbred Strains , Muscles/metabolism , RNA, Messenger/metabolism , Recurrence , Spleen/metabolism , Time Factors
13.
J Immunol ; 156(11): 4363-8, 1996 Jun 01.
Article in English | MEDLINE | ID: mdl-8666808

ABSTRACT

By 24 h after mechanical trauma to the cerebral cortex, astroglial reaction begins and injury sites are infiltrated by activated mononuclear phagocytes derived from blood-borne monocytes and endogenous microglia. There is little information about cellular interactions between astrocytes and leukocytes during this process. We previously showed that murine astrocytes produce chemokines including monocyte chemoattractant protein-1 (MCP-1) during experimental autoimmune encephalomyelitis. In this study, we asked whether astrocytes produce MCP-1 in the absence of immune mediated inflammation. To address this question, we analyzed the time course and cellular source of MCP-1 in mouse brain after penetrating mechanical injury, with particular focus on early time points before histologic detection of infiltrating mononuclear phagocytes. We observed sharply increased steady state levels of MCP-1 mRNA within 3 h after nitrocellulose membrane stab or implant injury to the adult mouse brain, and MCP-1 protein elevations were documented at 12 h postinjury. In situ hybridization combined with immunohistochemistry for the glial fibrillary acidic protein astrocyte marker showed that astrocytes were the cellular source of MCP-1 mRNA at these early time points after mechanical brain injury. Stab injury to the neonatal brain evoked neither MCP-1 expression nor astrogliosis. These results demonstrate that chemokine gene expression comprises one component of the astrocyte activation program. The data are consistent with a role for MCP-1 in the central nervous system inflammatory response to trauma.


Subject(s)
Astrocytes/immunology , Brain Injuries/immunology , Chemokine CCL2/genetics , Animals , Animals, Newborn , Astrocytes/metabolism , Brain/immunology , Brain/metabolism , Brain Injuries/genetics , Brain Injuries/metabolism , Female , Gene Expression , Male , Mice , RNA, Messenger/genetics , RNA, Messenger/metabolism , Wounds, Penetrating/genetics , Wounds, Penetrating/immunology , Wounds, Penetrating/metabolism
14.
Am J Pathol ; 148(3): 889-96, 1996 Mar.
Article in English | MEDLINE | ID: mdl-8774143

ABSTRACT

Reactive astrogliosis is a prominent pathological feature of multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis. It is characterized by hypertrophy of astrocytes with increased content of glial fibrillary acidic protein (GFAP.) Studies of reactive astrocytes in acute experimental autoimmune encephalomyelitis have been complicated by the observation that the diffuse increase in GFAP immunohistochemical staining at the onset of central nervous system inflammation does not parallel the gradual increase in GFAP content probably because tissue edema enhances GFAP immunostaining. To characterize changes in GFAP expression, we performed in situ hybridization at 3- to 7-day intervals during the course of acute murine experimental autoimmune encephalomyelitis. We found a biphasic course of GFAP expression: an early phase of astrocyte reaction surrounding perivascular inflammatory cuffs and submeningeal infiltrates at the onset of central nervous system inflammation and clinical disease and a later phase of increased GFAP mRNA expression in regions of demyelination during resolution of inflammation and clinical improvement. IP-10, a member of a family of proinflammatory chemoattractant cytokines called chemokines, was expressed by astrocytes in a similar distribution as those expressing increased GFAP mRNA in the early phase of inflammation but was no detected in astrocytes in the later phase of activation. These results indicate that location and function of reactive astrocytes may vary during the course of immune-mediated demyelination.


Subject(s)
Astrocytes/physiology , Encephalomyelitis, Autoimmune, Experimental/metabolism , Glial Fibrillary Acidic Protein/genetics , RNA, Messenger/metabolism , Acute Disease , Animals , Female , Glial Fibrillary Acidic Protein/metabolism , Immunohistochemistry , In Situ Hybridization , Mice , Mice, Inbred Strains , Time Factors
15.
Brain Behav Immun ; 9(4): 315-30, 1995 Dec.
Article in English | MEDLINE | ID: mdl-8903849

ABSTRACT

Central nervous system (CNS) expression of two chemokine mRNAs, encoding monocyte chemoattractant protein-1 (MCP-1) and IFN-gamma-inducible protein (IP-10), was previously shown to be closely related to the onset of clinical signs of murine experimental autoimmune encephalomyelitis (EAE). Chemokine mRNAs accumulated in a striking, transient burst within astrocytes, near inflammatory leukocyte infiltrates. It remained unclear if chemokines functioned to initiate leukocyte entry into CNS tissues, or to amplify the intrathecal inflammatory reaction. To address this issue, we determined the expression of chemokine mRNAs at the earliest evidence of CNS immune-mediated inflammation. For these experiments, mice were sacrificed in pairs at varying times after immunization. Only one member of each pair was symptomatic for EAE at the time of sacrifice. Symptom presence correlated well with histological inflammation at the time of sacrifice. RNA was prepared from two CNS sites, brain and spinal cord, and expression of chemokine mRNAs was analyzed by a sensitive and quantitative reverse transcriptase/polymerase chain reaction dot-blot hybridization assay. CNS expressions of MCP-1 and IP-10 gene were correlated tightly with histological inflammation; indeed, chemokine expression was never detected in the absence of leukocyte infiltrates. In situ hybridizations showed that astrocytes expressed chemokine transcripts. These findings provide new information about mechanisms controlling chemokine mRNA expression during immune-mediated inflammation in EAE and are consistent with a role for chemokines as amplifiers of CNS inflammatory reactions.


Subject(s)
Autoimmune Diseases/genetics , Central Nervous System/metabolism , Chemokine CCL2/biosynthesis , Chemokines, CXC , Chemotaxis, Leukocyte , Cytokines/biosynthesis , Encephalomyelitis, Autoimmune, Experimental/genetics , Gene Expression Regulation , RNA, Messenger/biosynthesis , Animals , Astrocytes/metabolism , Base Sequence , Chemokine CCL2/genetics , Chemokine CXCL10 , Cytokines/genetics , Female , Liver/metabolism , Mice , Mice, Inbred Strains , Molecular Sequence Data
16.
Int J Dev Neurosci ; 13(3-4): 153-65, 1995.
Article in English | MEDLINE | ID: mdl-7572272

ABSTRACT

In this paper, we discuss the potential involvement of a new family of cytokines, termed chemokines, in CNS inflammatory pathology. Chemokines are a family of proinflammatory cytokines which are able to stimulate target-cell-specific directional migration of leukocytes. Because of this feature, chemokines may be potent mediators of inflammatory processes. We have previously reported observations indicating that chemokines may be involved in the process of lesion formation during autoimmune inflammation within CNS, and, in particular, are likely participants in the process of influx of inflammatory cells into the CNS parenchyma. We observed also that mechanical injury of brain and subsequent post-traumatic inflammation may in part be mediated by chemokines. Chemokines undoubtedly co-operate with cell-associated adhesion molecules during recruitment of leukocytes from blood to CNS. The sequential expression of soluble and membrane-bound signals for leukocyte migration is an intricate process that can be interrupted by a variety of strategies. Our data suggest that chemokines may represent a promising target for future therapy of inflammatory conditions, including CNS inflammation resulting from varied insults.


Subject(s)
Central Nervous System/physiology , Chemokines/physiology , Animals , Central Nervous System/metabolism , Chemokines/metabolism , Humans , Inflammation/metabolism , Inflammation/pathology
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