Antibiofilm activity of cell-free supernatants of vaginal isolates of Corynebacterium amycolatum against Pseudomonas aeruginosa and Klebsiella pneumoniae.

Biofilm formation is an important factor in the development of antibiotic resistance and chronic infection. In this study, we demonstrated that the cell-free supernatant of vaginal isolates of C. amycolatum caused a reduction in biofilm formation, destroyed the preformed biofilms, altered the cell surface properties and reduced the production of exopolysaccharides in clinical isolates of P. aeruginosa и Kl. pneumoniae. Microscopic observations showed that P. aeruginosa and Kl. pneumoniae biofilm formed small clusters scattered over the surface after treatment with cell-free supernatant of C. amycolatum ICIS 99, in contrast to the dense aggregates observed in controls, as well as the flat, scattered, and unstructured biofilm architecture after treatment of preformed biofilms cell-free supernatant. The cells were flat and relatively unstructured. Based on these results, we hypothesize that C. amycolatum likely produces secondary metabolites with antimicrobial activity and utilizes a similar mechanism of action to bacteriocins and/or biosurfactants. The data obtained open the prospect of studying the metabolic profile of the cell-free supernatant of C. amycolatum to understand the nature and mechanism of the detected antibacterial action and provide further support for the probiotic potential of C. amycolatum vaginal isolates.

Genome sequence of Corynebacterium amycolatum ICIS 99 isolated from human vagina reveals safety and beneficial properties.

Corynebacterium amycolatum ICIS 99 was isolated from vaginal smears of healthy women and showed promising results in antimicrobial screenings. Here, we report the draft genome sequence of this strain and analyze its main features to assess its safety and useful properties. The genome is 2,532,503 bp long and contains 2186 CDSs with an average G + C content of 59.0%. Analyses of the ICIS 99 genome revealed the absence of true virulence factors. The genome contains genes involved in the synthesis of secondary metabolites and bacteriocins of the class sactipeptide. In the genome of ICIS 99, we identified a large number of genes responsible for adaptation and survival in the vaginal environment, including acid and oxidative stress resistance genes. The genomic information of ICIS 99 provides a basis for understanding the safety and useful properties of ICIS 99 and for considering it as a potential probiotic strain. The whole genome shotgun project has been deposited at DDBJ/ENA/GenBank under the accession number JAIUSU000000000.

Genome Characterization and Probiotic Potential of Corynebacterium amycolatum Human Vaginal Isolates.

The vaginal microbiome of healthy women contains nondiphtheria corynebacteria. The role and functions of nondiphtheria corynebacteria in the vaginal biotope are still under study. We sequenced and analysed the genomes of three vaginal C. amycolatum strains isolated from healthy women. Previous studies have shown that these strains produced metabolites that significantly increased the antagonistic activity of peroxide-producing lactic acid bacteria against pathogenic and opportunistic microorganisms and had strong antimicrobial activity against opportunistic pathogens. Analysis of the C. amycolatum genomes revealed the genes responsible for adaptation and survival in the vaginal environment, including acid and oxidative stress resistance genes. The genes responsible for the production of H2O2 and the synthesis of secondary metabolites, essential amino acids and vitamins were identified. A cluster of genes encoding the synthesis of bacteriocin was revealed in one of the annotated genomes. The obtained results allow us to consider the studied strains as potential probiotics that are capable of preventing the growth of pathogenic microorganisms and supporting colonisation resistance in the vaginal biotope.

Draft Genome Sequence of the Vaginal Isolate Corynebacterium amycolatum ICIS 9.

Corynebacterium amycolatum ICIS 9 was isolated from a vaginal smear of a healthy woman. Here, we report the draft genome sequence of C. amycolatum ICIS 9, which will be useful for further studies of specific genetic features of this strain and for understanding its probiotic properties.

Draft Genome Sequence of Corynebacterium amycolatum Strain ICIS 53 Isolated from a Female Urogenital Tract.

This report describes the draft genome sequence of Corynebacterium amycolatum strain ICIS 53, isolated from the reproductive tract of a healthy woman. The size of the genome was 2,460,257 bp (58.98% G+C content). Annotation revealed 2,173 coding sequences, including 2,076 proteins, 7 rRNA genes, and 53 tRNA genes.