ABSTRACT
In contrast to defibrinated blood, cells isolated from heparinized blood give lower T-lymphoproliferative responses. Such responses can be also suppressed by addition of heparin to lymphocyte cultures. Platelets inhibit T cell proliferation and B cell differentiation, but their interactions with heparin may produce contrasting effects on different immune functions.
Subject(s)
Antibody Formation , Blood Platelets/physiology , Heparin/pharmacology , Isoantigens/immunology , Lymphocyte Activation , T-Lymphocytes/immunology , Blood Platelets/drug effects , Humans , Leukocytes, Mononuclear/drug effects , Leukocytes, Mononuclear/immunology , Lymphocyte Activation/drug effects , Lymphocyte Culture Test, Mixed , T-Lymphocytes/drug effectsABSTRACT
A case of large granular lymphocyte lymphocytosis with neutropenia was studied as evidenced by peripheral lymphocytosis of cells having typical morphology and profound neutropenia. Surface markers analysis revealed that almost all cells were CD8+ and their subpopulation DR+. The cells had strong spontaneous and inducible suppressor functions in vitro. Moreover, their ADCC activity was strong but NK activity very weak.