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1.
J Oral Rehabil ; 45(11): 837-844, 2018 Nov.
Article in English | MEDLINE | ID: mdl-29926505

ABSTRACT

In 2013, consensus was obtained on a definition of bruxism as repetitive masticatory muscle activity characterised by clenching or grinding of the teeth and/or by bracing or thrusting of the mandible and specified as either sleep bruxism or awake bruxism. In addition, a grading system was proposed to determine the likelihood that a certain assessment of bruxism actually yields a valid outcome. This study discusses the need for an updated consensus and has the following aims: (i) to further clarify the 2013 definition and to develop separate definitions for sleep and awake bruxism; (ii) to determine whether bruxism is a disorder rather than a behaviour that can be a risk factor for certain clinical conditions; (iii) to re-examine the 2013 grading system; and (iv) to develop a research agenda. It was concluded that: (i) sleep and awake bruxism are masticatory muscle activities that occur during sleep (characterised as rhythmic or non-rhythmic) and wakefulness (characterised by repetitive or sustained tooth contact and/or by bracing or thrusting of the mandible), respectively; (ii) in otherwise healthy individuals, bruxism should not be considered as a disorder, but rather as a behaviour that can be a risk (and/or protective) factor for certain clinical consequences; (iii) both non-instrumental approaches (notably self-report) and instrumental approaches (notably electromyography) can be employed to assess bruxism; and (iv) standard cut-off points for establishing the presence or absence of bruxism should not be used in otherwise healthy individuals; rather, bruxism-related masticatory muscle activities should be assessed in the behaviour's continuum.


Subject(s)
Bruxism/classification , Bruxism/diagnosis , Masticatory Muscles/physiopathology , Sleep/physiology , Wakefulness/physiology , Bruxism/etiology , Consensus , Diagnosis, Differential , Electromyography , Humans , Polysomnography
2.
J Dent Res ; 95(4): 416-22, 2016 Apr.
Article in English | MEDLINE | ID: mdl-26758381

ABSTRACT

The role of masticatory muscle activation on pain in temporomandibular muscle and joint disorders (TMJD) is controversial. This single-group, prospective panel study examined the relationships among masticatory muscle tension, emotional distress, and TMJD pain in a sample of 7,023 observations obtained from 171 individuals using longitudinal multilevel modeling. Three main hypotheses were tested. The first posited that emotional distress and muscle tension directly influenced pain (hypothesis 1a: Distress → TMJD Pain; hypothesis 1b: Muscle Tension → TMJD Pain). The second posited that emotional distress directly influenced muscle tension (Distress → Muscle Tension), and the third posited that the effect of emotional distress on pain was mediated by muscle tension (Distress → Muscle Tension → TMJD pain). We also examined the fit of the data to possible alternative models. All the data used in this study were collected via an experience sampling methodology. The fit of the preferred models was better than that of the alternative models, with the preferred models explaining large proportions of the data, especially for level 2 variance (hypothesis 1a = 41% variance; hypothesis 1b = 69% variance; hypothesis 2 = 48% variance). In the mediation model, the addition of muscle tension to the model reduced the impact of emotional distress. The findings support a causal role for masticatory muscle tension in TMJD pain. Clinically, the results suggest that addressing tension and other oral parafunctions in those diagnosed with TMJDs should be an important part of the conservative, noninvasive care of individuals diagnosed with the myofascial pain or arthralgia of TMJD.


Subject(s)
Facial Pain/physiopathology , Facial Pain/psychology , Masticatory Muscles/physiopathology , Stress, Psychological , Temporomandibular Joint Disorders/physiopathology , Temporomandibular Joint Disorders/psychology , Adult , Female , Humans , Linear Models , Longitudinal Studies , Male , Muscle Tonus/physiology , Pain Measurement , Prospective Studies , Risk Factors
3.
Psychiatriki ; 26(1): 38-44, 2015.
Article in English | MEDLINE | ID: mdl-25880382

ABSTRACT

The quest for existential meaning constitutes a universal phenomenon traditionally manifested in official religions (religiosity) or personal modes of transcendence (spirituality). Religiosity and spirituality have been found to be associated with a variety of mental health and illness parameters. In the last decades there is an increasing number of publications with interesting results on the relationship between religiosity and mental health, both on a theoretical and a clinical level. Recent research suggests the presence of clinically important interactions between religious beliefs and mental health, although the exact nature of the associations remains unclear. The aim of the present study is to investigate subjective health status in relation to specific dimensions of religiosity and spirituality in Greek students; 202 students of the faculty of Theology of the University of Athens were interviewed using the Brief Multidimensional Measurement of Religiousness/Spirituality (BMMRS), which assesses the dimensions of "daily spiritual experiences", "meaning", "values/beliefs", "forgiveness", "private religious practices", "religious/spiritual coping", "religious support", "religious/ spiritual history", "commitment", "organizational religiousness", and "religious preferences". Subjective health status was measured by the General Health Questionnaire (GHQ-28) which examines four areas of health in the following sub-scales: (a) somatic symptoms, (b) anxiety and insomnia, (c) social dysfunction and (d) severe depression. Pearson correlations coefficients and linear regression analyses were used to estimate the associations of GHQ-28 subscales with religiosity dimensions. High scores in each dimension of BMMRS corresponded to a low level of religiosity. The dimension of "daily spiritual experiences" was positively correlated with the subscales of anxiety/ insomnia, social dysfunction and severe depression, while the dimension of "values/beliefs" with social dysfunction and severe depression and the dimension of "forgiveness" with all GHQ-28 subscales. The "organizational religiousness" dimension was positively correlated with anxiety/ insomnia, while overall self-ranking with social dysfunction and severe depression. Additionally, the dimension of "meaning" had a negative correlation with somatic symptoms. Moreover, in the multiple linear regression analyses, "meaning" was independently negative associated with somatic symptoms (p=0.032), whilst "daily spiritual experiences" were positively associated with anxiety/insomnia (p=0.023). Also, "values/beliefs and the overall self-ranking were positively associated with social dysfunction (p=0.026), (p=0.01) and "daily spiritual experiences", "values/beliefs", "forgiveness", as well as the overall self-ranking with severe depression (p=0.03), (p=0.01), (p=0.017), (p=0.009). Certain religiosity dimensions ("daily spiritual experiences", "values/beliefs", "forgiveness" and "organizational religiousness") were correlated with lower morbidity, in accordance to previous reports in different populations, whereas "meaning" was correlated with more somatic symptoms.


Subject(s)
Adaptation, Psychological , Depressive Disorder , Religion , Spirituality , Adult , Depressive Disorder/diagnosis , Depressive Disorder/epidemiology , Depressive Disorder/psychology , Diagnostic Self Evaluation , Female , Greece/epidemiology , Health Status Disparities , Humans , Male , Mental Health/statistics & numerical data , Psychometrics/methods , Self-Assessment , Social Adjustment , Students/psychology , Students/statistics & numerical data
4.
Folia Morphol (Warsz) ; 74(1): 100-5, 2015.
Article in English | MEDLINE | ID: mdl-25792403

ABSTRACT

BACKGROUND: There is no consensus in the literature as to which point of the radial artery (RA) is the safest to attempt vascular access. The purpose of this study was to measure the diameter, tortuosity and branching patterns of the distal RA. MATERIALS AND METHODS: 140 cadaveric RAs (66 male, 74 female) were dissected and measured. The external diameter of the RA was measured at 2 cm increments starting at the radial styloid process (SP), moving proximally. The location and degree of 2-dimensional arterial tortuosity were recorded if > 35 degrees. Branches of the RA were recorded with respect to their distance from the SP. RESULTS: We observed that the right RA significantly increased in diameter at distances beyond 4 cm proximal from the radial SP, regardless of the sex of the individual. This increase in size was not noted on the left RA's. Muscular artery branches of the distal RA were noted on average 1.82 cm proximal from the SP. Clinically significant tortuosity was present on average 3.47 cm proximal from the radial SP. The left RA did not significantly change in size along its course, but its statistically similar diameter when compared to the right RA allows us to make a recommendation this is applicable bilaterally. CONCLUSIONS: Our data suggests that regardless of gender, vascular access of the RA could be safely performed at distances greater than 4 cm from the SP to yield a vessel with a larger diameter, less tortuosity, and fewer branches.

5.
Int J Obes (Lond) ; 38(7): 912-8, 2014 Jul.
Article in English | MEDLINE | ID: mdl-24732146

ABSTRACT

OBJECTIVE: The objective of this study is to examine associations between aspects of the environment in school neighborhoods and childhood body mass index percentile (BMIp). METHODS: Trained medical students visited 46 elementary schools in the Kansas City metropolitan area to conduct medical screenings that included the height and weight measurements of 12 118 boys and girls 4-12 years of age in the academic year 2008-2009. For the same time period, aspects of the built environment in a 2-mile radius around each school was obtained from the Walkscore database. Other environmental characteristics (for example, population change) of these areas were also obtained from various sources. Hierarchical linear modeling was used to estimate the associations between neighborhood- and individual-level factors and BMIp. RESULTS: Population size along with the number of fast-food restaurants and grocery stores were positively associated with BMIp, whereas population change along with the number of parks and fitness centers were inversely associated with BMIp. CONCLUSIONS: After considering individual-level factors and the random effects of schools, environmental elements of school neighborhoods predict childhood BMIp. This study offers evidence of the health influence of school neighborhoods in a way that can inform neighborhood redevelopment efforts.


Subject(s)
Diet , Exercise , Pediatric Obesity/prevention & control , Residence Characteristics , School Health Services/organization & administration , Social Environment , Body Mass Index , Child , Child, Preschool , Choice Behavior , Environment Design , Female , Food Preferences , Health Education , Humans , Kansas/epidemiology , Linear Models , Male , Pediatric Obesity/epidemiology , Prevalence , Restaurants , Schools , Socioeconomic Factors
6.
J Oral Rehabil ; 40(1): 2-4, 2013 Jan.
Article in English | MEDLINE | ID: mdl-23121262

ABSTRACT

To date, there is no consensus about the definition and diagnostic grading of bruxism. A written consensus discussion was held among an international group of bruxism experts as to formulate a definition of bruxism and to suggest a grading system for its operationalisation. The expert group defined bruxism as a repetitive jaw-muscle activity characterised by clenching or grinding of the teeth and/or by bracing or thrusting of the mandible. Bruxism has two distinct circadian manifestations: it can occur during sleep (indicated as sleep bruxism) or during wakefulness (indicated as awake bruxism). For the operationalisation of this definition, the expert group proposes a diagnostic grading system of 'possible', 'probable' and 'definite' sleep or awake bruxism. The proposed definition and grading system are suggested for clinical and research purposes in all relevant dental and medical domains.


Subject(s)
Bruxism , Consensus , Bruxism/classification , Bruxism/diagnosis , Diagnosis, Differential , Electromyography , Humans , Movement , Physical Examination , Polysomnography , Sleep Bruxism/classification , Sleep Bruxism/diagnosis , Surveys and Questionnaires , Wakefulness
7.
Mutat Res ; 634(1-2): 156-62, 2007 Dec 01.
Article in English | MEDLINE | ID: mdl-17719836

ABSTRACT

The potential reactivity and structural properties of oxiranes (epoxides) are advantageous when considering polymers for medical devices. However, epoxy compounds are widely known to have genotoxic properties. The objective of the study was to evaluate the cytotoxicity and primary DNA damage effects induced by oxiranes and siloranes, silicon containing oxiranes. The siloranes, Ph-Sil, Tet-Sil, and Sil-Mix and the oxiranes Cyracure UVR-6105 and 1,3-bis[2-(2-oxiranylmethyl) phenoxy]pentane (OMP-5) were dissolved in organic solvents and dilutions containing less than 0.5% solvent were used in biological assays. The concentration that reduced the viability of 50% (TC(50)) of L929 cells was measured using the MTT assay and guided the selection of subtoxic doses for evaluation of DNA damage. Ph-Sil was more cytotoxic than OMP-5, Cyracure UVR-6105 and Sil-Mix. However, the TC(50) value of Tet-Sil could not be determined due to its poor solubility. DNA damage was evaluated in the sister chromatid exchange (SCE) assay with CHO cells, and the alkaline comet assay with L929 cells. In contrast to the siloranes, the oxiranes exhibited significant increases (p>0.05) in SCE frequencies and DNA migration relative to their solvent controls. Our findings support previous reports that siloranes have low genotoxic potential and can be suitable components for development of biomaterials.


Subject(s)
Cell Survival/drug effects , DNA Damage/drug effects , Ethylene Oxide/analogs & derivatives , Ethylene Oxide/toxicity , Silicon Compounds/toxicity , Animals , CHO Cells , Comet Assay , Cricetinae , Cricetulus , Epoxy Resins/toxicity , Ethylene Oxide/chemistry , Mice , Sister Chromatid Exchange
8.
Cephalalgia ; 27(6): 542-9, 2007 Jun.
Article in English | MEDLINE | ID: mdl-17441972

ABSTRACT

To assess the diagnostic and behavioural overlap of headache patients with temporomandibular disorders (TMD), individuals recruited from the general population with self-described headaches were compared with non-headache controls. The examination and diagnostic procedures in the Research Diagnostic Criteria (RDC) for TMD were applied to both sets of subjects by a blinded examiner. Following their examination, subjects used experience sampling methods to obtain data on pain, tooth contact, masticatory muscle tension, emotional states and stress. Results showed that a significantly higher proportion of the headache patients received an RDC/TMD diagnosis of myofascial pain than non-headache controls. Headache patients also reported significantly more frequent and intense tooth contact, more masticatory muscle tension, more stress and more pain in the face/head and other parts of the body than non-headache controls. These results are similar to those reported for TMD patients and they suggest that headache patients and TMD patients overlap considerably in diagnosis and oral parafunctional behaviours.


Subject(s)
Headache/diagnosis , Headache/physiopathology , Temporomandibular Joint Disorders/diagnosis , Temporomandibular Joint Disorders/physiopathology , Adult , Bruxism/physiopathology , Facial Pain/etiology , Facial Pain/physiopathology , Female , Humans , Male , Medical History Taking , Physical Examination
9.
Dentomaxillofac Radiol ; 36(3): 138-42, 2007 Mar.
Article in English | MEDLINE | ID: mdl-17463097

ABSTRACT

OBJECTIVES: To compare the diagnostic accuracy of conventional film, unenhanced direct digital and inversion grayscale direct digital imaging in the detection of approximal caries. METHODS: 150 approximal surfaces of extracted permanent molars and premolars were selected for the study on the basis of varying lesion depth. The teeth were radiographed using Ektaspeed Plus film; digital images were made with a Schick CMOS-APS sensor. 7 examiners evaluated 58 randomized images of each modality. Histological sectioning of the teeth was used to verify the presence and extent of decay. RESULTS: No significant difference was found between the diagnostic accuracies of the three imaging modalities (P=0.226). Analysis of the diagnostic accuracy of the three modalities on lesion depth showed no statistically significant interaction; however, the main effect of the lesion depth was significant (P<0.001, eta(2)=0.936). CONCLUSIONS: The overall diagnostic accuracy of the three modalities in the detection of approximal carious lesions was comparable. All three modalities performed poorly in the detection of enamel lesions.


Subject(s)
Dental Caries/diagnostic imaging , Radiography, Dental/methods , Analysis of Variance , Bicuspid/diagnostic imaging , Humans , Likelihood Functions , Molar/diagnostic imaging , ROC Curve , Radiography, Dental, Digital , Reproducibility of Results , Statistics, Nonparametric , X-Ray Film
10.
J Oral Rehabil ; 34(2): 97-104, 2007 Feb.
Article in English | MEDLINE | ID: mdl-17244231

ABSTRACT

Interocclusal splints may be an effective modality in the management of temporomandibular disorders (TMD), but there is little evidence regarding the mechanism by which splints work. This study tested the hypothesis that pain reduction produced by splints is associated with reduction in parafunctional activity. In a two-group, single-blinded randomized clinical trial, patients diagnosed with myofascial pain received full coverage hard maxillary stabilization splints. Patients were instructed to maintain or avoid contact with the splint for the 6 weeks of active treatment. Patients who decreased the intensity of tooth contact were expected to show the greatest alleviation of pain, and those who maintained or increased contact were expected to report lesser reductions in pain. Experience-sampling methodology was used to collect data on pain and parafunctional behaviours at pre-treatment and during the final week of treatment. Patients were reminded approximately every 2 h by pagers to maintain/avoid contact with the splint. The amount of change in intensity of tooth contact accounted for a significant proportion of the variance in pain change scores. Patients who reduced tooth contact intensity the most reported greater relief from pain. Splints may produce therapeutic effects by reducing parafunctional activities associated with TMD pain.


Subject(s)
Facial Pain/prevention & control , Motor Activity , Occlusal Splints , Temporomandibular Joint Disorders/therapy , Adolescent , Adult , Aged , Facial Pain/etiology , Female , Humans , Kansas , Male , Middle Aged , Pain Measurement , Single-Blind Method , Temporomandibular Joint Disorders/complications , Treatment Outcome
11.
J Oral Rehabil ; 30(6): 573-7, 2003 Jun.
Article in English | MEDLINE | ID: mdl-12787453

ABSTRACT

To test the hypothesis that a flat plane interocclusal appliance affects the electromyographic (EMG) activity of the temporalis and masseter muscles in pain-free individuals, maxillary splints were fabricated for 20 individuals who reported no history, signs or symptoms of myofascial pain or arthralgia as determined by two trained, independent examiners. Subjects were instructed to establish light tooth contact, maximum clenching, and moderate clenching with/without the splint in place (as determined by random assignment) while EMG data from the left and right temporalis and masseter muscles were recorded. A 5-min biofeedback training session to relax the masticatory muscles was followed by a repetition of the tooth contact/clenching tasks with/without the splint in place. With the splint in place, the activity of the temporalis muscles decreased for all tasks, significantly for the left and right temporalis under maximal clenching and for the right temporalis under moderate clenching. In contrast, the activity of the masseter muscles increased under light and moderate clenching (significantly for the left masseter under moderate clenching) and decreased slightly under maximal clenching. The effectiveness of interocclusal appliances may be due to mechanisms other than redistribution of adverse loading.


Subject(s)
Bruxism , Electromyography , Masseter Muscle/physiology , Occlusal Splints , Temporal Muscle/physiology , Temporomandibular Joint Disorders/physiopathology , Bruxism/physiopathology , Humans , Occlusal Splints/standards
12.
J Dent Res ; 82(5): 367-71, 2003 May.
Article in English | MEDLINE | ID: mdl-12709503

ABSTRACT

Unpolymerized dental monomers can leach out into the oral biophase and are bioavailable for metabolism. We hypothesize that metabolites would be less toxic than parent monomers. We first identified the formation of metabolites from bisphenol F diglycidyl ether (BFDGE) and Bisphenol A glycidyl methacrylate (BISGMA) after their exposure to liver S9 fractions. Then, the metabolites and parent compounds were subjected to in vitro cytotoxicity, mutagenicity, and estrogenicity studies. Bisphenol A bis(2,3-dihydroxypropyl) ether and bisphenol F bis(2,3-dihydroxypropyl) ether were the hydroxylated metabolites of BISGMA and BFDGE, respectively. Cytotoxicity against L929 cells showed that the metabolites were significantly (p < 0.05) less cytotoxic than the parent monomers. Only BFDGE was mutagenic in the Ames assay with strain TA100 of Salmonella typhimurium. Parent and metabolite compounds did not stimulate estrogen-dependent MCF-7 cell proliferation above solvent controls. These results indicated that the hydroxylated metabolites were non-mutagenic, non-estrogenic, and less cytotoxic than their parent monomers.


Subject(s)
Bisphenol A-Glycidyl Methacrylate/pharmacokinetics , Bisphenol A-Glycidyl Methacrylate/toxicity , Dental Materials/metabolism , Dental Materials/toxicity , Epoxy Compounds/pharmacokinetics , Epoxy Compounds/toxicity , Animals , Benzhydryl Compounds , Biocompatible Materials/metabolism , Biocompatible Materials/toxicity , Cells, Cultured/drug effects , Estrogens, Non-Steroidal/pharmacology , Humans , Hydroxylation , Inactivation, Metabolic , L Cells/drug effects , Materials Testing , Mice , Microsomes, Liver/metabolism , Mutagenicity Tests , Toxicity Tests
13.
Orthod Craniofac Res ; 5(3): 161-5, 2002 Aug.
Article in English | MEDLINE | ID: mdl-12194665

ABSTRACT

OBJECTIVES: To test the hypothesis that there is no significant difference in clinical bracket failure between the 3M APC Mini Twin bracket system and the Transbond XT adhesive during orthodontic treatment. DESIGN: A randomized, split mouth, prospective clinical trial. SETTING AND SAMPLE POPULATION: The Department of Orthodontics and Dentofacial Orthopedics at University of Missouri, Kansas City. Twenty-nine subjects who were treatment planned to receive comprehensive orthodontics using 0.018 slot appliances. EXPERIMENTAL VARIABLE: The adhesive pre-coated brackets (APC) system was randomly assigned to one side of the mouth and the Transbond XT was assigned to the opposite side. OUTCOME MEASURE: The absence of any of the brackets at the time of a regular or emergency orthodontic appointment. RESULTS: There was no significant difference (P > 0.05) in bracket failure between the 3M APC Mini Twin bracket system and the Transbond XT adhesive at any time period (90, 180, or 365 days). The failure rate of brackets on premolars was higher than on incisors or canines. CONCLUSION: APC Mini Twin bracket system and Transbond XT adhesive are equally effective in maintaining brackets clinically. Inexperienced operators had an overall 7.5% bond failure 12 months following bracket placement.


Subject(s)
Acrylic Resins/chemistry , Bisphenol A-Glycidyl Methacrylate/chemistry , Dental Bonding , Orthodontic Appliance Design , Orthodontic Brackets , Resin Cements/chemistry , Adolescent , Adult , Bicuspid , Chi-Square Distribution , Child , Cuspid , Equipment Failure , Humans , Incisor , Prospective Studies , Statistics as Topic , Surface Properties
14.
Dent Mater ; 18(5): 413-21, 2002 Jul.
Article in English | MEDLINE | ID: mdl-12175581

ABSTRACT

OBJECTIVES: Visible light cure oxirane/polyol resins of Cyracure UVR-6105 with pTHF-250 has been previously shown useful for development of dental composites. This oxirane/polyol (4016) in combination with other oxiranes were formulated into composites (4016E, 4016G and 4016GB) containing 72.9-74.9% quartz filler. The main objective of the study was to evaluate some of the physical properties and the biocompatibility of the composites. RESULTS: PhotoDSC analysis of composites demonstrated twice the enthalphy values of Z100 (31J/g). Composites 4016E and 4016G showed compressive strengths similar to Z100 (337+/-35Mpa), P>0.05. Discs of composite 4016E, containing Epon 825 oxirane (E), and composite 4016G containing Araldite GY 281 oxirane (G) were non-cytotoxic (-) while the composite 4016GB, containing G and Ebecryl 1830 (B), was mildly (+) cytotoxic to L929 cells in the agar diffusion assay. Seven-day extracts of 4016GB composite were cytotoxic while extracts of 4016E and 4016G were less cytotoxic to L929 cells in the MTT assay. Extracts were obtained from 7 day incubations of composite (3 cm(2) surface area/ml) in acetone or ethanol/saline (1:20) at 37 degrees C. All composite extracts were non-mutagenic to Ames strains TA100, TA98, TA97a and TA1535. The overall results with composite 4016GB suggest that leachable components were cytotoxic but non-mutagenic. With the exception of oxirane components, G and E, the oxirane Cyracure UVR-6105 and other components were non-mutagenic. From cytotoxicity studies, the photoinitiator, Sarcat CD 1012, was the most cytotoxic (TC(50)=14 microM) component. Components G (TC(50)=17 microM), E (TC(50)=50 microM) and B (TC(50)=151 microM) were significantly (p < 0.05) more cytotoxic than Cyracure UVR-6105 (1488 microM) and the polyol, pTHF-250 (TC(50)=6072 microM). SIGNIFICANCE: Favorable results obtained with composites 4016G and 4016E indicates that suitable oxirane/polyol formulations can be designed and optimized for development of dental composites with acceptable mechanical properties and biocompatibility. However, leachable analysis of extracts obtained from longer incubation periods is needed before final conclusions could be drawn about the leachability of oxirane components.


Subject(s)
Biocompatible Materials/chemistry , Biocompatible Materials/toxicity , Composite Resins/chemistry , Composite Resins/toxicity , Ethylene Oxide/toxicity , Animals , Area Under Curve , Cell Survival/drug effects , Chemical Phenomena , Chemistry, Physical , Electrophoresis, Agar Gel , Epoxy Resins/toxicity , L Cells/drug effects , Lethal Dose 50 , Materials Testing , Mice , Mutagenicity Tests , Polymers/toxicity , Solubility
15.
Biochim Biophys Acta ; 1550(1): 100-6, 2001 Nov 26.
Article in English | MEDLINE | ID: mdl-11738092

ABSTRACT

The dental restorative monomer, BISGMA (2,2-bis[4-(2-hydroxy-3-methacryloxypropoxy)phenyl]propane), and bisphenol A diglycidyl ether (BADGE) increase the velocity of the reaction catalyzed by pancreatic cholesterol esterase (CEase, bovine). The metabolite of these monomers, bisphenol A bis(2,3-dihydroxypropyl) ether, and a common plasticizer, di-2-ethylhexyl phthalate (DEHP), also increase the velocity of CEase-catalyzed ester hydrolysis. BISGMA at concentrations of 1.5-8.0 microM increases the velocity to 126-169% of its value in the absence of BISGMA. Increasing BISGMA above 8 microM caused no further increase in velocity. BADGE at 7-25 microM increases the velocity to 112-205% of its value without BADGE. The metabolite of BISGMA and BADGE at concentrations of 2.0-7.1 microM increases the velocity to 103-113% of its value without metabolite. DEHP at concentrations of 0.52-4.3 microM increases the velocity to 108-187% of its value without DEHP. On the other hand, bisphenol A dimethacrylate is a competitive inhibitor of CEase, with a K(i) of 3.1 microM.


Subject(s)
Dentin-Bonding Agents/pharmacology , Epoxy Compounds/pharmacology , Methacrylates/pharmacology , Sterol Esterase/chemistry , Benzhydryl Compounds , Butyrates/pharmacology , Diethylhexyl Phthalate/pharmacology , Enzyme Activation/drug effects , Kinetics , Molecular Structure , Sterol Esterase/antagonists & inhibitors
16.
J Biomater Sci Polym Ed ; 12(1): 89-105, 2001.
Article in English | MEDLINE | ID: mdl-11334192

ABSTRACT

The objective of this study was to identify through quantum mechanical quantitative structure activity relationships (Q-QSARs) chemical structures in dental monomers that influence their mutagenicity. AMPAC, a semiempirical computer program that provides quantum mechanical information for chemical structures, was applied to three series of reference chemicals: a set of methacrylates, a set of aromatic and a set of aliphatic epoxy compounds. QSAR models were developed using this chemical information together with mutagenicity data (Salmonella TA 100, Ames Test). CODESSA, a QSAR program that calculates quantum chemical descriptors from information generated by AMPAC and statistically matches these descriptors with observed biological properties was used. QSARs were developed which had r2 values exceeding 0.90 for each study series. These QSARs were used to accurately predict the mutagenicity of BISGMA. a monomer commonly used in dentistry, and two epoxy monomers with developing use in dentistry, GY-281 and UVR-6105. The Q-QSAR quantum mechanical descriptors correctly predicted the level of mutagenicity for all three compounds. The descriptors in the correlation equation pointed to components of structure that may contribute to mutagenesis. The QSARs also provided 'dose windows' for testing mutagenicity, circumventing the need for extensive dose exploration in the laboratory. The Q-QSAR method promises an approach for biomaterials scientists to predict and avoid mutagenicity from the chemicals used in new biomaterial designs.


Subject(s)
Dental Enamel/chemistry , Mutagens , Dose-Response Relationship, Drug , Methacrylates/chemistry , Models, Chemical , Mutagenicity Tests , Quantum Theory , Software , Structure-Activity Relationship
17.
J Periodontol ; 72(2): 174-82, 2001 Feb.
Article in English | MEDLINE | ID: mdl-11288790

ABSTRACT

BACKGROUND: Relative few reports exist concerning healing of laser created osteotomies over an extended period of time. The purpose of this study was to evaluate long-term healing, from 21 to 63 days, of osteotomy defects in the rat tibia created with the Nd:YAG and CO2 in the presence of a surface cooling spray of air/water. METHODS: The experimental model consisted of 15 large Sprague-Dawley rats. Six treatment modalities were randomly distributed among 6 tibial recipient sites: 1) a negative control (no treatment); 2) a positive control (bur osteotomy); 3) CO2 laser at 5 W (860 J/cm2); 4) CO2 laser at 6 W (1,032 J/cm2); 5) Nd:YAG laser at 5 W (714 J/cm2); and 6) Nd:YAG laser at 7 W (1,000 J/cm2). All laser irradiation was delivered in the presence of a surface coolant consisting of air (15 psi) and sterile water. Five animals were sacrificed at each of 3 time intervals: 21, 35, and 63 days post-treatment. Multiple histologic sections from each treatment site were examined by light microscopy using hematoxylin and eosin Goldner's trichrome stains, and polarized light and evaluated for presence of a char layer, heat induced cracking, heat related alterations in cells or tissue matrix, and osseous regeneration. RESULTS: Healing was severely delayed in all laser treated sites compared to positive control sites. Of the laser treated sites, those irradiated by CO2 laser at 5 W (780 J/cm2) exhibited the greater amount of bone regeneration. At best, however, only a small percentage of sections from any of the laser treated specimens showed evidence of bone regeneration within the ablation defect regardless of the post-treatment time interval. CONCLUSIONS: Under the conditions of this study, the osseous healing response was severely delayed by CO2 and Nd:YAG laser irradiation of bone, even in the presence of a surface cooling spray of air/water.


Subject(s)
Bone and Bones/surgery , Laser Therapy , Air , Aluminum Silicates , Analysis of Variance , Animals , Bone Marrow/pathology , Bone Marrow/surgery , Bone Matrix/pathology , Bone Matrix/surgery , Bone Regeneration , Bone and Bones/pathology , Carbon Dioxide , Coloring Agents , Fluorescent Dyes , Hot Temperature , Longitudinal Studies , Male , Microscopy, Polarization , Models, Animal , Necrosis , Neodymium , Osteotomy/instrumentation , Osteotomy/methods , Random Allocation , Rats , Rats, Sprague-Dawley , Tibia/pathology , Tibia/surgery , Water , Wound Healing , Yttrium
18.
J Dent Res ; 80(9): 1789-92, 2001 Sep.
Article in English | MEDLINE | ID: mdl-11926234

ABSTRACT

Many reports have demonstrated inflammation after the placement of dental restorations. To explain this side-effect, we studied a biomarker in the inflammatory response. The intercellular adhesion molecule-1 (ICAM-1) is a key mediator for recruitment of leukocytes to the site of inflammation. Therefore, we investigated whether methacrylates (a BISGMA-based dental resin, BISGMA, and MAA) and Cyracure UVR 6105, an epoxy monomer, could alter ICAM-1 expression in unstimulated and TNF-alpha-stimulated endothelial cells. Six-well plates with monolayers of human umbilical vein cells, ECV 304 (ATCC CRL 1998), were exposed to TNF-alpha (1 ng/mL) in the presence and absence of subtoxic and TC50 doses of chemicals for 24 hrs at 37 degrees C/5% CO2. Several doses of TNF-alpha (0.5-2 ng/mL) were coincubated with 100 microL of undiluted aqueous dental resin extracts. Cells were harvested and stained with mAB FITC-conjugated anti-human ICAM-1 (CD54). ICAM-1 expression was measured by flow cytometry. Cells expressed basal levels of ICAM-1, which was up-regulated by TNF-alpha but was not changed by all samples studied. Except for UVR 6105, the methacrylates significantly decreased ICAM-1 expression in TNF-alpha-stimulated cells. These findings suggest that methacrylates may decrease the recruitment of leukocytes to sites of inflammation.


Subject(s)
Dental Materials/toxicity , Endothelium/drug effects , Intercellular Adhesion Molecule-1/biosynthesis , Resins, Synthetic/toxicity , Tumor Necrosis Factor-alpha/pharmacology , Analysis of Variance , Bisphenol A-Glycidyl Methacrylate/toxicity , Cell Line, Transformed , Cell Survival/drug effects , Composite Resins/toxicity , Cyclohexanecarboxylic Acids/toxicity , Dose-Response Relationship, Drug , Endothelium/cytology , Endothelium/metabolism , Humans , In Vitro Techniques , Leukocytes/drug effects , Methacrylates/toxicity , Silicon Dioxide/toxicity , Zirconium/toxicity
19.
J Contemp Dent Pract ; 2(1): 24-30, 2001 Feb 15.
Article in English | MEDLINE | ID: mdl-12167941

ABSTRACT

The purpose of the study was to compare the segmental measurement tool from the Shick Technologies CDR digital system with the conventional film radiography measurement technique to determine preoperative working length. Natural extracted human teeth with varying degrees of root curvature were used. All teeth were imaged using the Shick Technologies CDR direct digital system and conventional E-speed film. Measurements from digital radiography and conventional film were compared with measurements made directly from the endodontic files. The Wilks' Lambda multivariate test was used. The mean measurement of each modality was compared with the other and with the gold standard. The test showed statistically significant differences between the two modalities at p<0.05. The mean measurements for both modalities were not significantly different from the gold standard. The modality most closely approaching the gold standard was the conventional film.


Subject(s)
Dental Pulp Cavity/anatomy & histology , Dental Pulp Cavity/diagnostic imaging , Odontometry/methods , Radiography, Dental, Digital , Root Canal Preparation/instrumentation , Humans , Multivariate Analysis , Odontometry/instrumentation , Tooth Root/anatomy & histology , Tooth Root/diagnostic imaging , X-Ray Film
20.
J Prosthodont ; 10(4): 204-11, 2001 Dec.
Article in English | MEDLINE | ID: mdl-11781968

ABSTRACT

PURPOSE: The study investigated the effects of palatal depth and a resin anchoring system on the adaptation of denture base resin to the master cast after compression molding and heat polymerization. MATERIALS AND METHODS: Forty-eight virtually identical polymethyl methacrylate dentures were fabricated on master casts with either a deep or shallow palatal vault. One half of the master casts of each palate type were altered by the addition of anchoring holes along the posterior land area, as well as perpendicularly in the midsaggital area. Anchoring holes were made with a #8 round bur to a depth of 5 mm. Twenty-four hours after polymerization, the bases on their casts were sectioned at the posterior border and evaluated for degree of adaptation using a traveling microscope. Maladaptation at the interface of the denture base and master cast was measured at predetermined mediolateral locations. A split-plot analysis of variance (alpha = 0.05) was performed followed by a post-hoc Dunn Multiple Comparison Test. RESULTS: In general, depth of the palatal vault did not significantly influence denture palatal discrepancy (p =.0780), but the use of the anchoring system significantly reduced mean gap distances (p =.000). At lateral and midpalate locations, gap distances between the denture bases and their casts were reduced from approximately 0.3 mm to approximately 0.1 mm when the anchoring system was used. CONCLUSIONS: Mean gap distances for steep palate dentures were significantly less than shallow palate dentures at vestibule and lateral palate locations, and anchoring holes placed in an edentulous master cast along the posterior land area and at the midline significantly improved the adaptation of denture bases.


Subject(s)
Acrylic Resins/chemistry , Denture Bases , Denture Design , Palate , Analysis of Variance , Hot Temperature , Humans , Models, Dental , Polymers/chemistry , Polymethyl Methacrylate/chemistry , Pressure , Statistics as Topic , Surface Properties
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