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1.
Biomed Opt Express ; 9(4): 1948-1963, 2018 Apr 01.
Article in English | MEDLINE | ID: mdl-29675331

ABSTRACT

Ocular UV doses accumulate all-day, not only during periods of direct sun exposure. The UV protection efficiency of three clear lenses was evaluated experimentally, validated by simulation, and compared to non-UV protection: a first spectacle lens with a tailored UV absorber, a second spectacle lens, minimizing UV back reflections, as well as a third spectacle lens, combining both. A tailored UV-absorber efficiently reduced overall UV irradiance to 7 %, whereas reduction of back-reflections still left UV irradiance at 42 %. Thus, clear lenses with a tailored UV absorber efficiently protect the eye from UV, supplementing sun glasses wear to an all-day protection scenario.

2.
J Biomed Opt ; 12(1): 014038, 2007.
Article in English | MEDLINE | ID: mdl-17343513

ABSTRACT

A highly parallel microarray scanner for functional genomic research using a dynamically reconfigurable holographic excitation and hyperspectral fluorescence detection is described. The light from two laser sources (405 and 532 nm) is split into an arbitrary number of focused spots on the biochip using a pair of spatial light modulators and a novel imaging system. The parallel optical scanner includes a hyperspectral detection unit with a high-sensitivity CCD camera to detect and analyze the emitted fluorescence spectra (from 430 to 800 nm) of all illuminated spots simultaneously. By using an xy scanner, the spectra of all spots on an entire array can be read out line by line.


Subject(s)
Holography/instrumentation , Microscopy, Confocal/instrumentation , Microscopy, Fluorescence, Multiphoton/instrumentation , Oligonucleotide Array Sequence Analysis/instrumentation , Equipment Design , Equipment Failure Analysis , Holography/methods , Microscopy, Confocal/methods , Microscopy, Fluorescence, Multiphoton/methods , Oligonucleotide Array Sequence Analysis/methods , Protein Array Analysis/instrumentation , Protein Array Analysis/methods , Reproducibility of Results , Sensitivity and Specificity
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