Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Type of study
Language
Publication year range
1.
J Infect Dis ; 214(3): 426-37, 2016 08 01.
Article in English | MEDLINE | ID: mdl-27234419

ABSTRACT

BACKGROUND: Bacillus Calmette-Guerin (BCG) vaccine is widely used for the prevention of tuberculosis, despite limited efficacy. Most immunological studies of BCG or Mycobacterium tuberculosis strains grow bacteria in the presence of detergent, which also strips the mycobacterial capsule. The impact of the capsule on vaccine efficacy has not been explored. METHODS: We tested the influence of detergent in cultures of BCG and M. tuberculosis strains on the outcome of vaccination experiments on mice and transcriptional responses on M. tuberculosis RESULTS: Vaccination of mice with encapsulated BCG promoted a more potent immune response relative to vaccination with unencapsulated BCG, including higher polysaccharide-specific capsule antibody titers, higher interferon γ and interleukin 17 splenic responses, and more multifunctional CD4(+) T cells. These differences correlated with variability in the bacterial burden in lung and spleen of mice infected with encapsulated or unencapsulated M. tuberculosis The combination of vaccination and challenge with encapsulated strains resulted in the greatest protection efficacy. The transcriptome of encapsulated M. tuberculosis was similar to that of starvation, hypoxia, stationary phase, or nonreplicating persistence. CONCLUSIONS: The presence of detergent in growth media and a capsule on BCG were associated with differences in the outcome of vaccination, implying that these are important variables in immunological studies.


Subject(s)
BCG Vaccine/immunology , Bacterial Capsules/immunology , Bacterial Capsules/metabolism , Culture Media/chemistry , Mycobacterium bovis/immunology , Mycobacterium bovis/metabolism , Adjuvants, Immunologic , Animals , Antibodies, Bacterial/blood , CD4-Positive T-Lymphocytes/immunology , Female , Gene Expression Profiling , Interferon-gamma/metabolism , Interleukin-17/metabolism , Mice, Inbred C57BL , Mycobacterium bovis/growth & development , Mycobacterium tuberculosis/genetics , Mycobacterium tuberculosis/growth & development , Mycobacterium tuberculosis/metabolism
SELECTION OF CITATIONS
SEARCH DETAIL
...