Subject(s)
Nicotiana/drug effects , Plants, Toxic , Polysorbates/toxicity , Reactive Oxygen Species/toxicity , Solvents/toxicity , Water Pollutants, Chemical/toxicity , Cells, Cultured , Electric Conductivity , Hydrogen-Ion Concentration , Paraquat/metabolism , Paraquat/toxicity , Peroxides/metabolism , Peroxides/toxicity , Polysorbates/metabolism , Reactive Oxygen Species/metabolism , Solvents/metabolism , Nicotiana/cytology , Nicotiana/metabolism , Water Pollutants, Chemical/metabolism , tert-ButylhydroperoxideABSTRACT
Acetyl-CoA carboxylase activities from whole blood and, in some of the experiments, from liver were found to be lower in biotin-deficient chicks compared with controls. In vitro stimulation of liver acetyl-CoA carboxylase activity by biotin appeared to be a better index for the evaluation of the biotin status than measuring the enzyme activity alone.
Subject(s)
Biotin/deficiency , Liver/enzymology , Acetyl-CoA Carboxylase/blood , Acetyl-CoA Carboxylase/metabolism , Animals , Biotin/blood , Chickens , Female , MaleABSTRACT
Pyruvate carboxylase activities of erythrocytes and liver preparations and their in vitro stimulation by biotin were used for the determination of the biotin status of chicks. Reasonable stability of the enzyme in erythrocytes was achieved when storing the erythrocytes deep-frozen in a glycerol-containing medium. Results of the activation assays in erythrocytes and liver are compared with biotin levels in feed, plasma and liver. The pyruvate carboxylase activation assay appears to be a useful tool for assessing the biotin status of chicks.
