ABSTRACT
Nitrogen dioxide's rats' inhalations with injections per os of pyrazole, amidopyrine and sodium nitrite lead to considerable increasing of endogenic N-nitrosodimethylamine formation, which had been determined by system gas chromatograph-thermal energetic analyser. This increasing essentially didn't depend on the rats' immunisation by vaccine BCG, which leads to the intensification of NO synthesis by peritoneal macrophages and others manifestations of their metabolic activation: increasing of creatine kinase and adenosine desaminase activities. It hadn't been brought to light the obvious dependent between changes of xanthine oxidase and xanthine dehydrogenase activities in the liver and blood serum and intensification of lipids peroxidation and also the amount of N-nitrosodimethylamine in the rats in the conditions of endogenic and exogenic nitrosation factors' influence.
Subject(s)
Dimethylnitrosamine/metabolism , Purines/metabolism , Animals , BCG Vaccine/pharmacology , Chromatography, Gas , Lipid Peroxidation , Liver/drug effects , Liver/enzymology , Liver/metabolism , Macrophages, Peritoneal/drug effects , Macrophages, Peritoneal/metabolism , Male , Nitrosation , Rats , Xanthine Dehydrogenase/blood , Xanthine Dehydrogenase/metabolism , Xanthine Oxidase/blood , Xanthine Oxidase/metabolismABSTRACT
The Ukrainian INEC Section is the most recently established chapter of the European Institute of Ecology and Cancer (INEC). We herewith review its organization and goals, membership, scientific platform, and areas of scientific activity.
Subject(s)
Ecology , Neoplasms/epidemiology , Societies, Medical/organization & administration , Humans , Societies, Medical/trends , UkraineABSTRACT
Inhalation of nitrogen dioxide (NO2) by mice administered orally amidopyrine (AP) and sodium nitrite resulted in increased biosynthesis of N-nitrosodimethylamine (NDMA), as determined by analysis using gas chromatography with thermal energy analyzer detector. These results were also confirmed indirectly in chronic experiments on rats using the system of biomarkers of NDMA formation (single-stranded DNA liver damages, alanine-aminotransferase, glutathione-S-transferase, and liver S9 fraction activity). The inhibition of NDMA metabolism by 4-methylpyrazol (4-MP) administration increases the sensitivity of NDMA biosynthesis assay in frozen whole-mouse powder. The results confirm that NO2 can serve as the precursor of nitrosamines.
Subject(s)
Carcinogens/metabolism , Dimethylnitrosamine/metabolism , Liver/metabolism , Nitrogen Dioxide/administration & dosage , Administration, Inhalation , Administration, Oral , Alanine Transaminase/metabolism , Aminopyrine/administration & dosage , Aminopyrine/metabolism , Animals , Anti-Inflammatory Agents, Non-Steroidal/administration & dosage , Anti-Inflammatory Agents, Non-Steroidal/metabolism , Biomarkers , DNA Damage/drug effects , Drug Combinations , Glutathione Transferase/metabolism , Male , Mice , Mice, Inbred C57BL , Mutagens/administration & dosage , Mutagens/metabolism , Rats , Sodium Nitrite/administration & dosage , Sodium Nitrite/metabolismABSTRACT
The effect of carcinogenic N-nitrosoamines (NA), NA-producing tetramethylthiuram disulfide (TMTD) pesticide, and of various doses of nitrogen fertilizers (90, 180, and 270 kg/ha) on the forming and vital functions of microbiocenosis of light gray forest soil was studied. The quantity, nitrifying activity, and gas production of nitrifying bacteria increased under the influence of NA. The nitrosodimethylamine (NDMA) and the nitrosodibutilamine (NDBA) changed the correlation between the number of soil-inhabiting fungi and bacteria so that the former prevailed. The greatest effect was observed on the twentieth day of cultivation of the soil when high concentrations of NDMA (50 mcg/kg) and NDBA (105 mcg/kg) were used. The typical dominant species among the fungi was the phytotoxigenic Penicillium funiculosum. The nitrosationable pesticide tetramethylthiuram disulfide (TMTD) applied with nitrogen fertilizers in a dose of 180 and 270 kg/ha exerted an influence on the microbiocenosis of the soil similar to NA. The potato yield decreased and the ecology of microbiocenosis deteriorated under these conditions in a model vegetable experiment. It is suggested that the nitrosoamines and their precursors may change the balance of nitrogen, decrease the yield of plants, and deteriorate the ecological balance.
Subject(s)
Nitrosamines/pharmacology , Soil Microbiology , Solanum tuberosum/drug effects , Thiram/pharmacology , Dimethylnitrosamine/pharmacology , Models, Biological , Solanum tuberosum/growth & development , Time FactorsABSTRACT
On the primary rat lung cell cultures, a study was made of the transforming action of sodium nitrite (NN) and amidopyrine combination with the control for formation of carcinogen--N-nitrosodimethylamine (NDMA) in growth medium. The manifestation of transformation was registered by appearance of morphological changes and multilayer growth foci. As criteria for evaluation after 48 hour treatments with NDMA, NN and AP, were used DNA-synthetizing activity of cells, mitotic index, frequency of mitoses pathology, monolayer density. The effects of transforming dose of NN alone and in combination with AP were the same. But malignization (tumor development in newborn rats in the points of cell suspension inoculation) took place only after administration of NN in combination with AP, when carcinogen was formed. Theophylline decreased the action of NN-AP combination.
