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1.
Article in Russian | MEDLINE | ID: mdl-377871

ABSTRACT

Experiments in a number of biological models showed that the cultures of E. coli isolated from the urine of children with pyelonephritis had a varied spectrum of pathogenic properties. Histologically confirmed pyelonephritis induced by intravenous infection in CBA mice, treated with 5% glucose by the method of Montgomerie et al., correlated with the bacterial adhesiveness to the epithelium and the interference with capillary permeability, registered in experiments on the pulmonary model with Evans blue used for control. The criterion for the development of pyelonephritis in mice, which was based, in the opinion of Mintogemerie et al., on the positive results, indicating the presence of the infecting agent in the culture obtained by inoculation with the samples of urine and kidney tissue, was found to be insufficient, as only 28 out of 45 cultures of E. coli isolated from the kidneys coincided with histologically confirmed cases of pyelonephritis.


Subject(s)
Capillary Permeability , Escherichia coli Infections/etiology , Escherichia coli/pathogenicity , Pyelonephritis/etiology , Animals , Disease Models, Animal , Escherichia coli Infections/microbiology , Evans Blue , Mice , Mice, Inbred CBA , Pyelonephritis/microbiology , Time Factors
2.
Article in Russian | MEDLINE | ID: mdl-339627

ABSTRACT

A study was made of 268 cultures isolated from the urine of 263 children suffering from pyelonephritis. Of the total number of different cultures E. coli constituted 79.3 percent; the percentage of the rest varied from 5.2 to 0.4. Examination of 87 urinocultures of E. coli isolated from sick children with the specific immune response showed that the majority of bacterial signs (urease activity, capacity to produce alpha-hemolysin to utilize saccharose and raffinose, to synthesize colicine) failed to correlate with their pyelopathogenicity. The reference to individual serological groups also failed to serve as a sufficient foundation for the separation of these microbes into individual nephropathogenic or pyelopathogenic groups. In experiments with 3H-glucose labeled bacteria there was revealed a marked adhesive capacity in 94 percent of E. coli strains towards the epithelial cells of the RH strain. A positive radioactive label failed to correlate with the presence in E. coli of common pili and with the bacterial agglutinability with the sera K88, K99, and KH-III. The latter pointed to the presence of a factor of unknown nature in the nephropathogenic E. coli strains imparting adhesive properties to bacteria.


Subject(s)
Bacteriuria/microbiology , Escherichia coli Infections/microbiology , Escherichia coli/isolation & purification , Pyelonephritis/microbiology , Antibodies, Bacterial/isolation & purification , Child , Escherichia coli/immunology , Escherichia coli Infections/urine , Humans , Pyelonephritis/urine , Serotyping
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