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Rapid Commun Mass Spectrom ; 9(10): 897-901, 1995.
Article in English | MEDLINE | ID: mdl-7670154

ABSTRACT

We report the sequence analysis of an oligodeoxyribonucleotide by electrospray mass spectrometry (ES-MS). For full sequence analysis of the oligomer, d(CGTAGCTACG), an enzyme digestion step followed by off-line high performance liquid chromatography was used. For ES-MS analysis, introducing the sample in an imidazole buffer with 70% acetonitrile increased the electrospray ion currents and helped reduce sodium-adduct ion peaks.


Subject(s)
Oligonucleotides/analysis , Sequence Analysis, DNA/methods , Chromatography, High Pressure Liquid , Mass Spectrometry , Molecular Sequence Data , Oligonucleotides/chemical synthesis , Phosphoric Diester Hydrolases/chemistry , Snake Venoms/chemistry , Spectrophotometry, Ultraviolet
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