Effect of light colour and photoperiod on biomass growth and phycocyanin production by Synechococcus PCC 6715.

The effect of light colour and light regime on growth and production of the thermostable C-phycocyanin (PC) by the thermophilic cyanobacterium Synechococcus 6715 in the tubular photobioreactor has been analysed. The highest specific growth rate (1.918 d-1) and biomass concentration (5.11 gVS ⋅L-1) were observed under constant illumination of the red light. However, the PC concentration in volatile solids (e.g blue light 30.68 ± 0.8 mgPC⋅gVS-1 PP and 21.7 ± 1 mgPC⋅gVS-1 CI) as well as per photobioreactor unit volume (e.g red light 122.66 ± 2.28 mgPC⋅L-1 PP and 74.71 ± 8.43 mgPC⋅L-1 PP) was higher in the 16L:8D photoperiod. The obtained PC purity was higher in the case of photoperiod (≈1.5). PCC6715 lacks genes encoding phycoerythrins what suggests T1 type of pigmentation. Although changes in biomass pigmentation were not significant, the strain was able to adapt its photosystem what can be used in the optimization of PC production by application of different light colours.

Mathematical modeling of the integrated process of mercury bioremediation in the industrial bioreactor.

The mathematical model of the integrated process of mercury contaminated wastewater bioremediation in a fixed-bed industrial bioreactor is presented. An activated carbon packing in the bioreactor plays the role of an adsorbent for ionic mercury and at the same time of a carrier material for immobilization of mercury-reducing bacteria. The model includes three basic stages of the bioremediation process: mass transfer in the liquid phase, adsorption of mercury onto activated carbon and ionic mercury bioreduction to Hg(0) by immobilized microorganisms. Model calculations were verified using experimental data obtained during the process of industrial wastewater bioremediation in the bioreactor of 1 m³ volume. It was found that the presented model reflects the properties of the real system quite well. Numerical simulation of the bioremediation process confirmed the experimentally observed positive effect of the integration of ionic mercury adsorption and bioreduction in one apparatus.