ABSTRACT
Samples of four types of particulate bone matrix derivatives were prepared and surgically inserted into standardized critical-sized defects in calvariae of Long-Evans rats. Implantation of demineralized bone matrix (DBM), bone regenerative matrix, and two types of particulate chemosterilized, antigen-extracted, autolyzed, allogeneic (AAA) bone from endochondral (eAAA) and intramembranous (iAAA) sources will result in the regeneration of bone in orthotopic skull defects. The four preparations were tested in orthotopic, 8-mm calvarial wounds to compare the quantity of new bone that formed 28 days postimplantation. Quantitative computer imaging was used to measure roentgenographic gray levels and bone volume of new trabeculae (calcified plus osteoid). All experimental bone matrix derivatives produced more bone volume than the nontreated control wounds. Bone volume regenerated from iAAA was significantly less than eAAA and DBM-regenerated bone.
