Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 4 de 4
Filter
Add more filters










Database
Language
Publication year range
1.
J Allergy (Cairo) ; 2012: 490905, 2012.
Article in English | MEDLINE | ID: mdl-23118775

ABSTRACT

Sublingual immunotherapy (SLIT) has been considered to be a painless and efficacious therapeutic treatment of allergic rhinitis which is known as type I allergy of nasal mucosa. Nevertheless, its mechanisms need to be further investigated. In this study, we constructed an effective murine model of sublingual immunotherapy in allergic rhinitis, in which mice were sublingually administered with ovalbumin (OVA) followed by intraperitoneal sensitization and nasal challenge of OVA. Sublingually treated mice showed significantly decreased specific IgE responses as well as suppressed Th2 immune responses. Sublingual administration of OVA did not alter the frequency of CD4(+)CD25(+) regulatory T cells (Tregs), but led to upregulation of Foxp3- and IL-10-specific mRNAs in the Tregs of cervical lymph nodes (CLN), which strongly suppressed Th2 cytokine production from CD4(+)CD25(-) effector T cells in vitro. Furthermore, sublingual administration of plasmids encoding the lymphoid chemokines CCL19 and CCL21-Ser DNA together with OVA suppressed allergic responses. These results suggest that IL-10-expressing CD4(+)CD25(+)Foxp3(+) Tregs in CLN are involved in the suppression of allergic responses and that CCL19/CCL21 may contribute to it in mice that received SLIT.

2.
Adv Otorhinolaryngol ; 72: 103-6, 2011.
Article in English | MEDLINE | ID: mdl-21865703

ABSTRACT

Sublingual immunotherapy has been considered to be a painless and effective therapeutic treatment for allergic rhinitis, and is known as type 1 allergy of the nasal mucosa. So far, its mechanism of action has been elucidated employing peripheral blood serum and lymphocytes in an antigen-specific fashion. Because of the limitations in sampling human materials, there is still controversy among many reports between clinical efficacy and laboratory data. Therefore, its mechanism of action needs to be investigated further by using promising animal models such as rodents and monkeys. Bearing this in mind, in our present study, we successfully constructed an effective murine model for sublingual immunotherapy in allergic rhinitis in which mice were administered ovalbumin (OVA) sublingually followed by intraperitoneal sensitization and nasal challenge.


Subject(s)
Immunotherapy/methods , Nasal Mucosa/immunology , Ovalbumin/administration & dosage , Rhinitis, Allergic, Seasonal/therapy , Administration, Sublingual , Humans , Rhinitis, Allergic, Seasonal/immunology , Treatment Outcome
3.
J Immunol ; 186(7): 4253-62, 2011 Apr 01.
Article in English | MEDLINE | ID: mdl-21357262

ABSTRACT

In this study, we demonstrated a new airway Ag sampling site by analyzing tissue sections of the murine nasal passages. We revealed the presence of respiratory M cells, which had the ability to take up OVA and recombinant Salmonella typhimurium expressing GFP, in the turbinates covered with single-layer epithelium. These M cells were also capable of taking up respiratory pathogen group A Streptococcus after nasal challenge. Inhibitor of DNA binding/differentiation 2 (Id2)-deficient mice, which are deficient in lymphoid tissues, including nasopharynx-associated lymphoid tissue, had a similar frequency of M cell clusters in their nasal epithelia to that of their littermates, Id2(+/-) mice. The titers of Ag-specific Abs were as high in Id2(-/-) mice as in Id2(+/-) mice after nasal immunization with recombinant Salmonella-ToxC or group A Streptococcus, indicating that respiratory M cells were capable of sampling inhaled bacterial Ag to initiate an Ag-specific immune response. Taken together, these findings suggest that respiratory M cells act as a nasopharynx-associated lymphoid tissue-independent alternative gateway for Ag sampling and subsequent induction of Ag-specific immune responses in the upper respiratory tract.


Subject(s)
Antigens, Bacterial/administration & dosage , Lymphoid Tissue/immunology , Nasal Mucosa/immunology , Nasopharynx/immunology , Plant Lectins/administration & dosage , Turbinates/immunology , Administration, Inhalation , Animals , Antigens, Bacterial/immunology , Epithelial Cells/immunology , Epithelial Cells/microbiology , Epithelial Cells/ultrastructure , Lymphocyte Count , Lymphoid Tissue/microbiology , Lymphoid Tissue/ultrastructure , Mice , Mice, 129 Strain , Mice, Inbred BALB C , Mice, Knockout , Nasal Cavity/immunology , Nasal Cavity/microbiology , Nasal Cavity/ultrastructure , Nasal Mucosa/microbiology , Nasal Mucosa/ultrastructure , Nasopharynx/microbiology , Nasopharynx/ultrastructure , Plant Lectins/biosynthesis , Plant Lectins/immunology , Salmonella typhimurium/immunology , Streptococcus pyogenes/immunology , Turbinates/microbiology , Turbinates/ultrastructure , Ulex/immunology , Wheat Germ Agglutinins/immunology
4.
J Exp Med ; 206(11): 2351-64, 2009 Oct 26.
Article in English | MEDLINE | ID: mdl-19822644

ABSTRACT

The eye is protected by the ocular immunosurveillance system. We show that tear duct-associated lymphoid tissue (TALT) is located in the mouse lacrimal sac and shares immunological characteristics with mucosa-associated lymphoid tissues (MALTs), including the presence of M cells and immunocompetent cells for antigen uptake and subsequent generation of mucosal immune responses against ocularly encountered antigens and bacteria such as Pseudomonas aeruginosa. Initiation of TALT genesis began postnatally; it occurred even in germ-free conditions and was independent of signaling through organogenesis regulators, including inhibitor of DNA binding/differentiation 2, retinoic acid-related orphan receptor gammat, lymphotoxin (LT) alpha1beta2-LTbetaR, and lymphoid chemokines (CCL19, CCL21, and CXCL13). Thus, TALT shares immunological features with MALT but has a distinct tissue genesis mechanism and plays a key role in ocular immunity.


Subject(s)
Immunity/immunology , Lacrimal Apparatus/embryology , Lacrimal Apparatus/immunology , Lymphoid Tissue/embryology , Lymphoid Tissue/immunology , Organogenesis/immunology , Animals , Animals, Newborn , CD4-Positive T-Lymphocytes/immunology , Epithelium/immunology , Epithelium/ultrastructure , Epitopes/immunology , Immunization , Immunoglobulin A/immunology , Inhibitor of Differentiation Protein 2/metabolism , Lacrimal Apparatus/microbiology , Lacrimal Apparatus/ultrastructure , Lymphoid Tissue/microbiology , Lymphoid Tissue/ultrastructure , Lymphotoxin alpha1, beta2 Heterotrimer/metabolism , Mice , Nuclear Receptor Subfamily 1, Group F, Member 3 , Receptors, Retinoic Acid/metabolism , Receptors, Thyroid Hormone/metabolism
SELECTION OF CITATIONS
SEARCH DETAIL
...