ABSTRACT
The narrow genetics of most crops is a fundamental vulnerability to food security. This makes wild crop relatives a strategic resource of genetic diversity that can be used for crop improvement and adaptation to new agricultural challenges. Here, we uncover the contribution of one wild species accession, Arachis cardenasii GKP 10017, to the peanut crop (Arachis hypogaea) that was initiated by complex hybridizations in the 1960s and propagated by international seed exchange. However, until this study, the global scale of the dispersal of genetic contributions from this wild accession had been obscured by the multiple germplasm transfers, breeding cycles, and unrecorded genetic mixing between lineages that had occurred over the years. By genetic analysis and pedigree research, we identified A. cardenasii-enhanced, disease-resistant cultivars in Africa, Asia, Oceania, and the Americas. These cultivars provide widespread improved food security and environmental and economic benefits. This study emphasizes the importance of wild species and collaborative networks of international expertise for crop improvement. However, it also highlights the consequences of the implementation of a patchwork of restrictive national laws and sea changes in attitudes regarding germplasm that followed in the wake of the Convention on Biological Diversity. Today, the botanical collections and multiple seed exchanges which enable benefits such as those revealed by this study are drastically reduced. The research reported here underscores the vital importance of ready access to germplasm in ensuring long-term world food security.
Subject(s)
Arachis/genetics , Crops, Agricultural/genetics , Seeds/genetics , Africa , Asia , Chromosome Mapping/methods , DNA, Plant/genetics , Genetic Markers/genetics , Genetic Variation/genetics , Genome, Plant/genetics , Hybridization, Genetic/genetics , Oceania , Plant Breeding/methods , Species SpecificityABSTRACT
PREMISE OF THE STUDY: The genetic bottleneck of polyploid formation can be mitigated by multiple origins, gene flow, and recombination among different lineages. In crop plants with limited origins, efforts to increase genetic diversity have limitations. Here we used lineage recombination to increase genetic diversity in peanut, an allotetraploid likely of single origin, by crossing with a novel allopolyploid genotype and selecting improved lines. METHODS: Single backcross progeny from cultivated peanut × wild species-derived allotetraploid cross were studied over successive generations. Using genetic assumptions that encompass segmental allotetraploidy, we used single nucleotide polymorphisms and whole-genome sequence data to infer genome structures. KEY RESULTS: Selected lines, despite a high proportion of wild alleles, are agronomically adapted, productive, and with improved disease resistances. Wild alleles mostly substituted homologous segments of the peanut genome. Regions of dispersed wild alleles, characteristic of gene conversion, also occurred. However, wild chromosome segments sometimes replaced cultivated peanut's homeologous subgenome; A. ipaënsis B sometimes replaced A. hypogaea A subgenome (~0.6%), and A. duranensis replaced A. hypogaea B subgenome segments (~2%). Furthermore, some subgenome regions historically lost in cultivated peanut were "recovered" by wild chromosome segments (effectively reversing the "polyploid ratchet"). These processes resulted in lines with new genome structure variations. CONCLUSIONS: Genetic diversity was introduced by wild allele introgression, and by introducing new genome structure variations. These results highlight the special possibilities of segmental allotetraploidy and of using lineage recombination to increase genetic diversity in peanut, likely mirroring what occurs in natural segmental allopolyploids with multiple origins.
Subject(s)
Arachis/genetics , Hybridization, Genetic , Polyploidy , Alleles , Genetic Variation , Homologous RecombinationABSTRACT
The occurrence of the armyworm Spodoptera albula (Walker) is registered for the first time attacking peanut in Tupã county (21° 56' 00" S; 50° 31' 00" W), São Paulo State, Brazil.
É registrada pela primeira vez a ocorrência de Spodoptera albula (Walker) atacando amendoim no município de Tupã (21° 56' 00" S; 50° 31' 00" W), no Estado de São Paulo, Brasil.
ABSTRACT
High mite infestations were observed during two consecutive years (1999 and 2000) in peanut fields of cultivar IAC-Caiapó, at the Adamantina Experiment Station of Instituto Agronômico de Campinas (IAC). The mites were identified as Tetranychus ogmophallos Ferreira & Flechtmann, and the symptoms consisted of an initial silver-like chlorosis on leaves, progressing to formation of a great ammount of web structures covering the plants. For a preliminary evaluation of the damage, two 1.000 m² plots were marked during the 1998/99 field growing. One was not treated with pesticide and the other was chemically sprayed for mite control. In both plots, pod harvesting was done at three intervals, and pod yield within each plot was estimated as the average of harvesting intervals. Comparison between treated and untreated plots indicated a 76,5 percent yield reduction due to mite infestation. T. ogmophallos has been previously known from Arachis pintoi, a wild peanut species, but no report of its occurrence on cultivated peanut (Arachis hipogaea) was known.
Altas infestações do ácaro Tetranychus ogmophallos Ferreira & Flechtmann foram observadas em plantios de amendoim cultivar IAC-Caiapó realizados na Estação Experimental de Adamantina, Estado de São Paulo, pertencente ao Instituto Agronômico de Campinas (IAC), durante dois anos agrícolas consecutivos (1998/99 e 2000). As infestações foram caracterizadas inicialmente pelo prateamento das folhas com posterior formação de grande quantidade de teia cobrindo as plantas. T. ogmophallos foi descrito a partir de exemplares coletados em Arachis pintoi e, até o momento, não havia sido registrado infestando amendoim cultivado. No plantio de 1998/99, duas áreas de 1000 m² foram demarcadas para a avaliação do dano causado pelo ácaro nas plantas de amendoim; em uma dessas áreas, o ácaro foi controlado quimicamente. Com base em três épocas distintas de colheita nas duas áreas, verificou-se redução de 76,5 por cento na média de produção de vagens.
