Development stage-dependent oxidative stress responses to the exposure to roundup original© in a neotropical annual killifish.

Herbicides are the most commonly applied pesticides in Brazil, specifically those based on glyphosate, and are used for different crops, near the habitats of annual killifish. Annual killifish presents a short life cycle with generally restricted geographic distribution. In this context, we evaluated the effect of the Roundup Original© (65, 130 and 260 µg. L-1 of glyphosate) herbicide on different development stages (adult-young and senile) of the annual killifish (Cynopoecilus sp.). We quantified the oxidative balance markers (superoxide dismutase, catalase, glutathione S-transferase, lipid peroxidation levels, and total proteins). We observed that the senile individuals presented 2-fold higher lipid peroxidation levels associated with the maintenance of superoxide dismutase and catalase activity levels even after exposure to the herbicide. However, senile subjects were negatively impacted by the exposure to formulations containing glyphosate, and this was related to a loss of glutathione S-transferase activity. Our research demonstrated that the established physiological markers and this species look promising for toxicology studies.

Biomarkers of oxidative stress in the post-embryonic characterization of the neotropical annual killifish.

Annual killifish are among the most remarkable extremophile species with the shortest vertebrate life span. Few studies have reported on the oxidative balance throughout their life cycle and its association to the natural aging process of these neotropical animals in a natural environment. We standardized and analyzed physiological markers related to the redox balance of the annual killifish (Cynopoecilus fulgens) throughout the post-embryonic life cycle (enzyme activity of Superoxide Dismutase, Catalase, Glutathione Peroxidase, and Glutathione S-transferase, as well as the determination of the levels of Lipoperoxidation, Carbonylated Proteins, and Total Proteins). We tested the influence of environmental variables on these biomarkers. Individuals were collected, including juveniles, adults, and seniles, in three sampling units around the Parque Nacional da Lagoa do Peixe, located in the Coastal Plain of Rio Grande do Sul. We observed that males and females used different physiological strategies of their redox balance during their life cycle, and their oxidative balance was influenced by their reproductive period and environmental variables (water temperature, abundance of predators, abundance of another sympatric annual killifish species, and abundance of C. fulgens). The population of each temporary pond presented different physiological responses to the adaptation of their life cycle, and there was an influence of environmental component as a modulator of this cycle. Our study offers reference values that will be useful for comparison in future research with short-lived organisms.