Systems genomics of salinity stress response in rice.

Populations can adapt to stressful environments through changes in gene expression. However, the role of gene regulation in mediating stress response and adaptation remains largely unexplored. Here, we use an integrative field dataset obtained from 780 plants of Oryza sativa ssp. indica (rice) grown in a field experiment under normal or moderate salt stress conditions to examine selection and evolution of gene expression variation under salinity stress conditions. We find that salinity stress induces increased selective pressure on gene expression. Further, we show that trans-eQTLs rather than cis-eQTLs are primarily associated with rice's gene expression under salinity stress, potentially via a few master-regulators. Importantly, and contrary to the expectations, we find that cis-trans reinforcement is more common than cis-trans compensation which may be reflective of rice diversification subsequent to domestication. We further identify genetic fixation as the likely mechanism underlying this compensation/reinforcement. Additionally, we show that cis- and trans-eQTLs are under different selection regimes, giving us insights into the evolutionary dynamics of gene expression variation. By examining genomic, transcriptomic, and phenotypic variation across a rice population, we gain insights into the molecular and genetic landscape underlying adaptive salinity stress responses, which is relevant for other crops and other stresses.

Vaccine inequity: a threat to Africa's recovery from COVID-19.

BACKGROUND: Vaccine inequity is a reality facing the Sub-Saharan Africa region as vaccine nationalism from high-income countries (HICs) leads to limited access to the lifesaving vaccines needed to end the pandemic. In Africa, a significant portion of the population has yet to be vaccinated against Covid-19; however, the barriers to accessing such vaccines, including capacity challenges, still persist despite the implementation of the COVAX facility meant to support the lower- and middle-income countries (LMICs) to boost vaccination. METHODS: This study involved a systemic narrative review where literature search was conducted using the NCBI's PMC and BMC databases based on defined keywords. Three authors were involved in the literature search and consensus was applied to settle disagreements and validate the findings. RESULTS: In this systematic narrative review, we report that vaccine nationalism remains a challenge for LMICs as HICs still hoard vaccines and even bypass COVAX to procure doses directly from the manufacturers. Factors that promote vaccine hesitancy in Africa include misinformation regarding the Covid-19 vaccine, a lack of trust in politicians and the pharmaceutical industry, and concerns about vaccine safety and efficacy. The policies implemented to enhance vaccine coverage in Africa, such as mandates, community engagement, and partnerships, all seek to promote equity of vaccination and ending Covid-19. CONCLUSION: Covid-19 vaccine inequity persists and contributes to prolonged pandemic in LMICs. In response, African governments have taken certain measures to enhance vaccine uptake but more needs to be done to address resistance to vaccines.

Healthcare providers in Nigerian hospitals and HIV-related stigma: a systematic review.

BACKGROUND: Elimination of barriers to identification of new HIV infections, treatment adherence and retention in care of people living with HIV/AIDS is vital to the attainment of WHO's ambitious vision 2030 of 90:90:90 for HIV/AIDS. However, HIV-related stigma, especially among health workers, has been widely documented as a serious threat to this project. This study explored the factors associated with the stigmatization of people living with HIV among healthcare workers in Nigerian hospitals. METHOD: Electronic literature search was conducted on eight databases using keywords and MeSH guidelines. Using the PRISMA protocol, studies published from 2003 to 2022 were retrieved and analyzed. RESULT: Of the 1481 articles identified, 9 met the inclusion criteria. All the included studies were conducted across 10 of the 36 states in Nigeria, with every geo-political zone in Nigeria represented by at least two studies. The overarching themes identified were attitude and beliefs (n = 7), knowledge of HIV/AIDS (n = 3), quality of care (n = 4), education and in-service training (n = 4), and health facility policies and procedures (n = 3). Factors associated with HIV-related stigma among healthcare workers varied by gender, healthcare settings, specialties of health workers, and the presence of institutional stigma reinforcements. Healthcare workers without recent in-service training on HIV/AIDS and those who work in hospitals without anti-HIV/AIDS stigma policies exhibited more HIV-related stigmatizing attitudes. CONCLUSION: Continuous in-service training of healthcare workers and the development of comprehensive stigma reduction interventions that will be reinforced with anti-HIV stigma policies in clinical settings may facilitate the attainment of national HIV prevention goals.

Vision-related quality of life amongst patients with low vision and blindness in a resource-limited country.

PURPOSE: To evaluate the significance of sociodemographic and clinical variables on the quality of life of low-vision patients in a tertiary hospital in Enugu, Nigeria, and also determine the causative ocular pathologies and their vision-related quality of life. METHODS: A cross-sectional study of adults 18 years and above with low vision/blindness. They were consecutively enrolled and clinically evaluated. The WHO-NEI-VFQ-25 questionnaire for quality of life was administered. Descriptive statistics were used to summarize variables. Kruskal-Wallis test, analysis of variance, and post hoc tests were done where appropriate. RESULTS: Of the 400 participants, 81% were above 40 years. Mean age was 57.27 ± 16.34 years. Males (52.0%) and females (48.0%). 225 (56.25%) had Moderately Severe Visual Impairment. Low vision/blindness negatively affected the different domains of quality of life significantly, except ocular pain and dependency. Glaucoma and Cataract were the major disease conditions that affected most of the domains significantly. These conditions when poorly managed, due to poor and limited health resources, progress to low vision/blindness. Of all the socio-demographic factors and clinical variables studied, only age and severity of low vision were significant predictors of quality of life. CONCLUSION: There is the need for prompt and proper treatment of low-vision patients and advocacy for the establishment of adequate and functional rehabilitation services. Quality of life which measures the overall impact of a disease on an individual was low in people with low vision and blindness. Low-vision services, which include rehabilitation, will provide comprehensive eye-care services and thus improve their quality of life.

Age and body stature, handgrip, and strength endurance analyses of elite weightlifters: boosting 1RM bench press

Abstract Aim: This study aims to characterize the body stature and age, handgrip, and endurance strength of elite male weightlifters, and ascertain the anthropometric features that can boost IRM bench press. Methods: Forty-four elite male weightlifters of different weight categories participated in this study. All participants were members of the Ebonyi state team in Nigeria. We assessed their body weight, height, arm span, arm girth, chest girth, hip girth, thigh girth, handgrip strength, 1RM, time for repetitions (TR), and the number of repetitions (NR) of bench press, using National Strength and Conditioning Association's guidelines. Results: 1RM bench press depends on waist and thigh girths, while the speed of bench press was associated with height and arm span of elite weightlifters. 1RM, NR, and TR of bench press depend on age, body weight, and BMI. Consistently, NR of bench press increase with age; range 18, 22 years, and body weight increases with age; range 28, 32 years, while handgrip strength reduces with age; range 38 years and above (p < 0.05). Conclusion: The strength of association of age, and BMI with strength endurance and 1RM bench press was robust and similar, but inconsistent with handgrip strength. It also suggests that enhancing the musculoskeletal structures of the thigh and waist could boost 1RM bench press while handgrip strength could not but reduces as age advances beyond 38 years.

Maternal and neonatal anthropometric analyses: Determining birth outcomes in low-risk pregnancies at Alex Ekwueme Federal University Teaching Hospital, Abakaliki.

OBJECTIVE: To assess the input of maternal and neonatal body physique anthropometries to birth outcomes. METHODS: A prospective study of 177 full-term births at Alex Ekwueme Federal Teaching Hospital Abakaliki from July to December, 2019. Maternal and neonatal anthropometric parameters and birth outcomes: mode of delivery and Apgar score were considered in this study. The measurements followed the guidelines outlined by the Institute of Medicine. RESULTS: The prevalence of low birth weight (6.21%), cesarean delivery (14.12%), and abnormal Apgar score (9.04%) was relatively low in the study. Birth weight was dependent on maternal age, weight, body mass index, percentage body fat, and waist and hip circumference (P < 0.05). Normal Apgar score at 1 min after birth of male neonates was dependent on maternal BMI and neonatal head circumference whereas abnormal Apgar score in male and female neonates was dependent on maternal age and waist:hip ratio, respectively (P < 0.05). CONCLUSION: Maternal and neonatal body physique anthropometries can be used to identify mothers and neonates at risk of having birth complications. This provided a practical alternative means to know the possible risk of undesirable birth outcomes with spontaneous vaginal delivery in low-risk pregnancies.

Cognitive-behavioural reflective training for improving critical thinking disposition of nursing students.

BACKGROUND/OBJECTIVE: The importance of critical thinking in improving treatment practices in, for instance, the nursing profession, cannot be overemphasized. Despite this importance, empirical studies have shown that helping strategies to train learners on critical thinking disposition are needed. Given this knowledge gap, this study investigated the impacts of cognitive-behavioral reflective training for improving the critical thinking disposition of nursing students. METHOD: Of all the students screened, a total of 167 participants were assigned to the treatment group and waitlisted control group. A researchers-developed training program aimed at improving critical thinking disposition was delivered in English language by therapists. RESULTS: Repeated-measures ANOVA showed that there was no significant difference between the critical thinking disposition of nursing students in the treatment and control groups as measured by CTDI-M at the posttest. At the posttreatment and follow-up measures, there were consistently improved impacts of CBRT on the critical thinking disposition of nursing students in Nigeria as measured by CTDI-M. CONCLUSION: Following the results, we concluded that cognitive-behavioral reflective training was beneficial and had sustained improvement in enhancing the critical thinking disposition of nursing students.

Impact of rational emotive behavioral therapy on personal value system of students with visual impairment: A group randomized control study.

BACKGROUND: Visually impaired students have been reported to struggle with value system and rash decision making process. This study examined the impact of rational emotive behavioral therapy (REBT) in reducing negative personal value system of visually impaired people. METHOD: A group-randomized trial design was adopted using 56 students with visual impairment. The participants received a value-based rational emotive behavior progamme and were assessed at 3 points using PVS and ABS-2-AV. RESULTS: The result of analysis of covariance showed that there was no significant difference between the treatment and control groups in initial personal value in Nigerian as measured by PVS. At the post-treatment the effect of V-REBP was significant in personal value and follow-up assessment respectively in favour of treatment group. CONCLUSION: We concluded that there is significant impact of rational emotive behavioral therapy on reduction of negative personal value system of students with visual impairment.

Effect of a critical thinking intervention on stress management among undergraduates of adult education and extramural studies programs.

OBJECTIVE: The objective of this study was to examine the effect of a critical thinking intervention (CTI) on stress management among undergraduates of adult education and extramural studies programs. METHOD: A total of 44 undergraduates were randomly sorted into experimental and waitlist control groups. We used the Perceived Stress Scale for data collection at the pre-test, post-test, and follow-up stages. We used unpaired t and paired t-tests to analyze the data collected. SPSS version 22.0 was used for the data analyses (SPSS Inc., Chicago, IL). RESULTS: It was shown that the CTI was effective in reducing the mean stress of the participants compared to the control group both in the post-test (t[42] = -22.453, P < .001) and follow-up periods (t[42] = -34.292, P < .001). There were statistically significant changes in the mean stress of participants in the experimental group from the pre-test to post-test phases (t[23] = 26.30, P = .000, r = .08], and from pre-test to follow-up(t[23] = 37.10, P = .000, r = .30). The mean stress of the participants in the experimental group from post-test to follow-up signified the sustained positive influence of the CTI on the mean stress (t[23] = 2.41, P = .000, r = .46) of the undergraduates. CONCLUSION: This study adds to the literature by showing that a CTI is a valuable strategy for stress reduction in a university environment. Given that the CTI demonstrated the ability to reduce stress among undergraduates enrolled in adult education and extramural studies programs, we hope that similar interventions will be adopted to manage and prevent stress among students in other departments and disciplines.

Chemical footprints of harmattan dust and traffic corridor particulates monitored at two environmentally distinct geopolitical zones in Nigeria.

Harmattan dust and traffic-related pollution have been a serious environmental concern in the West African sub-region. In order to further contribute to the understanding of ambient levels of atmospheric pollution and chemical composition in the region, this study monitored harmattan dust and traffic-related particulate matter at four locations across southwestern (Ile-Ife) and north-western (Zaria) geo-political zones of Nigeria. The collected samples were characterized for their chemical composition using Energy-Dispersive X-Ray Fluorescence spectrometer equipped with an optimized secondary target x-ray excitation conditions (Al, CaF2, Fe, Ge, Zr, Mo, Ag, Al2O3). The objectives are to assess spatio-temporal mass concentrations, chemical footprints, enrichment factors, elemental correlations, and ratios at all locations. The X-ray analytical method was validated with a NIST SRM 2783 air particulate standard, and detection limits for each chemical specie were determined. Validation results showed good reproducibility of the certified reference material with relative standard deviations of the elements much lower by about 1-13% than the corresponding reference values. Mass concentrations reached up to 2200 µgm-3 in the north and 1500 µgm-3 in the south. The range of mean concentration of crustal marker elements were Al (5-27 µgm-3), Si (5-856 µgm-3), Ca (0.78-13 µgm-3), and Fe (2-13 µgm-3), and were most abundant during the harmattan particularly in the southwestern region. Highest mean concentration values of 380, 810, and 420 ngm-3 were recorded for Cr, Cu, and Pb respectively at the traffic corridor which also recorded the highest enrichment factors. Black carbon and elemental concentrations contributed between 1 to 54% and 9 to 94% across the locations respectively. Backward trajectories of atmospheric flow over the locations showed two dominant sources; dust laden source from the Sahara desert and maritime flow over the Gulf of Guinea. This study found that chemical footprints (Al, Si, K, Ca, Ti, and Fe) of harmattan-related dust were more correlated (r2 between 0.88 and 0.99) than those attributed to dust re-suspension at the traffic location.

In vivo studies on the biochemical indices of Plasmodium berghei infected mice treated with Alstonia boonei leaf and root extracts.

BACKGROUND: A study on the biochemical indices of albino mice infected with Plasmodium berghei and treated with Alstonia boonei aqueous and ethanolic extracts was undertaken. METHODS: 216 males mice were randomly assigned to six treatment groups each containing six mice for both aqueous and ethanolic extracts experiments. P. berghei NK-65 was inoculated into the mice intraperitoneally and establishment of infection confirmed. Administration of extracts of was done after phytochemical and acute toxicity tests at varying concentrations, for both suppressive and curative tests. Blood samples collected by ocular puncturing were examined for the biochemical indices; ALT, AST, ALP, creatinine and total protein using the standard procedures. RESULTS: A. boonei extracts suppression of P. berghei in mice was comparable to the standard drug. Significantly higher (p<0.05) recovery of mice treated with A. boonei extracts was observed. The biochemical indices examined all had significantly (p<0.05) increased concentration after 7 days post-infection, except for total protein concentration which had no significant increase or decrease due to A. boonei extracts administration. CONCLUSION: The antiplasmodial potentials of A. boonei leaf and root extracts were dosage and duration-dependent, and have demonstrated satisfactory normalization of altered biochemical indices due to malaria.

Biomonitoring of environmental pollution in the vicinity of iron and steel smelters in southwestern Nigeria using transplanted lichens and mosses.

This study identified specific emission sources of atmospheric pollution in the vicinity of two secondary iron and steel smelting factories in Osun state, southwestern Nigeria, using transplanted biomonitors. A total of 120 biomonitors consisting of lichen and moss were grown under a controlled environment and later transplanted to the surroundings of each factory for monitoring of air pollutants for 3 months in both wet and dry seasons. The elemental contents (K, Ca, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Se, Br, Rb and Sr) of the biomonitors were determined by X-ray florescence (XRF) spectroscopy. The source identification was performed by applying positive matrix factorization (PMF) receptor modelling approach using the elemental data set from the two smelters. Among the measured elements, Fe had the highest average concentration in the lichen and moss samples as well as in both seasons. The average concentrations of Co, Ni, Cu, Zn, As and Br were low. The varying average elemental concentrations of lichen and moss reflect the pattern of impact of smelting on atmospheric airborne pollution around the factories. The four factors resolved by PMF and their respective contributions were metal processing (39.0%), Fe source (28.0%), crustal/soil (22.0%) and road dust (11.0%) for moss and Fe source (34.0%), crustal/soil (26.0%), coal combustion (25.0%) and road dust (15.0%) for lichen. The study showcases lichen and moss as cheaper and yet efficient uninterrupted monitoring tools of air pollution sources associated with iron and steel smelting industrial activities.

Near-field infrared nanospectroscopy and super-resolution fluorescence microscopy enable complementary nanoscale analyses of lymphocyte nuclei.

Recent super-resolution fluorescence microscopy (3D-Structured Illumination Microscopy, 3D-SIM) studies have revealed significantly altered nuclear organization between normal lymphocyte nuclei and those of classical Hodgkin's Lymphoma. Similar changes have been found in Multiple Myeloma (MM) nuclei, as well as in a premalignant condition, Monoclonal Gammopathy of Unknown Significance (MGUS). Using 3D-SIM, an increase in DNA-poor and DNA-free voids was evident in reconstructed 3D-SIM images of diseased nuclei at 40 nm pixel resolution (x,y: 40 nm, z: 80 nm). At best, far-field FTIR imaging yields spatially resolved images at ∼500 nm spatial resolution; however, near-field infrared imaging breaks the diffraction limit at a scale comparable to that of 3D-SIM, providing details on the order of 30 nm spatial resolution. We report here the first near-field IR imaging of lymphocyte nuclei, and far-field IR imaging results of whole lymphocytes and nuclei from normal human blood. Cells and nuclei were mounted on infrared-compatible substrates, including CaF2, undoped silicon wafers, and gold-coated silicon wafers. Thermal source far-field FTIR images were obtained with an Agilent-Cary 620 microscope, 15× objective, 0.62 NA and 64 × 64 array Focal Plane Array detector (University of Manitoba), or with a similar microscope equipped with both 15× and 25× (0.81 NA) objectives, 128 × 128 FPA and either thermal source or synchrotron source (single beam) infrared light at the Advanced Light Source (ALS), LBNL, Berkeley CA. Near-field IR spectra were acquired at the ALS, on the in-house SINS equipment, as well as with a Neaspec system, both illuminated with synchrotron light. Finally, some near-field IR spectra and images were acquired at Neaspec GmbH, Germany. Far-field IR spectra of normal cells and nuclei showed the characteristic bands of DNA and proteins. Near-field IR spectra of nuclei showed variations in bands assigned to protein and nucleic acids including single and double-stranded DNA. Near-field IR images of nuclei enabled visualization of protein and DNA distribution in spatially-resolved chromosome territories and nuclear pores.

Antidiabetic and antihyperlipidemic potential of ethanol extract of Salacia lehmbachii stem bark in alloxan-induced diabetic rats.

Background Salacial lehmbachii stem bark is used traditionally for the treatment of diabetes mellitus and its associated complications. Treatment of diabetes is necessary to reduce these complications. Methods In this study, the antidiabetic and antihyperlipidemic potential of S. lehmbachii ethanol stem bark extract was evaluated in alloxan-induced diabetic rats at a dose of 100 mg/kg, 200 mg/kg, and 400 mg/kg p.o. daily for 21 days. Blood glucose levels, serum total cholesterol (TC), triglycerides (TG), high density lipoprotein (HDL), low density lipoprotein (LDL), and very low density lipoprotein (VLDL) were assessed in the animals. Results Treatment of alloxan-induced diabetic rats with S. lehmbachii stem bark extract showed significant (p<0.01) reduction in blood glucose levels when compared with diabetic control. The elevated levels of serum cholesterol, triglycerides, LDL, and VLDL were significantly (p<0.01) reduced by S. lehmbachii stem bark extract, while the level of HDL significantly (p<0.01) increased. Conclusions The results obtained suggest that S. lehmbachii stem bark extract has the potential to treat diabetes condition and hyperlipidemic disorders.

Loneliness, life satisfaction and psychological distress among out-of-school adolescents in a Nigerian urban city.

Loneliness is the feeling of distress that arises when an individual perceives his or her social relationships as being less satisfying than what is desired. Life satisfaction is a global assessment of an individual's quality of life based on his chosen criteria. This descriptive cross sectional study assessed loneliness, life satisfaction and psychological distress among 480 out of school adolescents using the UCLA loneliness scale, the Satisfaction with life scale (SWLS) and the General Health questionnaire. Reasons for out of school are poverty (45.0%), eager to do business (30.0%) and death of parents (20.0%). 25.8% experienced frequent loneliness while 24.2% had psychological distress. Findings were discussed in line with literature reviewed and recommendations made.

Antidiarrheal and antimicrobial activities of the ethanol extract from the Icacina senegalensis root bark.

BACKGROUND: The Icacina senegalensis root bark is traditionally used for the treatment of gastrointestinal disorders in Nigeria. To date, no scientific study has substantiated or refuted this claim. METHODS: The antidiarrheal and antimicrobial activities of the ethanol root bark extract were investigated in rats and against some selected diarrhea-causing microorganisms. RESULTS: The extract significantly decreased the frequency of castor oil-induced diarrhea, and inhibited the masses and volumes of intestinal fluid accumulation in the castor oil-induced enteropooling method. The distance travelled by the charcoal meal was also decreased by the extract gastrointestinal transit method. The extract also strongly inhibited the growth of some selected microorganisms. CONCLUSIONS: The ethanol extract of the I. senegalensis root bark showed antidiarrheal activity, thus justifying its long folkloric use in diarrhea treatment. The extract also demonstrated antimicrobial activity against selected diarrheal causing organisms.

Psychosocial correlates of perceived stress among undergraduate medical students in Nigeria.

OBJECTIVES: To assess the prevalence and factors associated with perceived stress among medical students. METHODS: A cross-sectional study of students (n=623) selected across eight medical schools in Nigeria. A structured questionnaire obtained socio-demographic characteristics, alcohol use (Alcohol Use Disorders Identification Test), other psychoactive drug use (Drug Abuse Screening Test), anxiety/depression symptoms (Hospital Anxiety Depression Scale) and stress (Perceived Medical School Stress Scale). We performed bivariate analysis using the chi-squared test, t-test and one-way ANOVA, with multiple regression analysis for multivariate testing in analysing the data. RESULTS: Most students reported experiencing medical school stress. Female participants were more likely to perceive medical school as competitive (t(621)=1.17, p=0.003), less likely to see medical school as a threat (t(621)=-2.70, p=0.01) or worry about finances (t(621)=-4.80, p=0.001). Nearly a quarter; 21.3% (n=133) and 28.6% (n=178) reported depression and anxiety symptoms respectively. Approximately 4.2% (n=26) were dependent on alcohol, while 14.1% (n=88) had 'low-risk use' for other psychoactive substances. In the multiple regression model, lack of finance (B=2.881, p=0.001), weak adherence to religious faith (B=2.376, p=0.001), anxiety symptoms (B=-2.231, p=0.002), problematic alcohol use (B=5.196, p=0.001) and choice of study influenced by parents (B=-3.105, p=0.001) were predictors of greater perceived stress. CONCLUSIONS: Medical students in Nigeria report high levels of stress. Incorporating stress reduction strategies in the medical curriculum, and the input of students in providing feedback regarding the methods and styles of undergraduate medical education is required.

Graves' Thyrotoxicosis Presenting as Schizophreniform Psychosis: A Case Report and Literature Review.

Psychosis, as the first presentation of thyrotoxicosis, is extremely rare. Consequently, it is often misdiagnosed as a primary psychiatric disorder, especially in developing countries with poor healthcare facilities. Owing to the high level of illiteracy and lack of knowledge, it is fairly common to ascribe many illnesses to spiritual attacks in Nigeria and other African countries, especially when the disease is rarely seen or is associated with psychiatric manifestations. Herein, we present the case of a teenage female Nigerian and review the literature on this subject.

Gemtuzumab ozogamicin in acute myeloid leukemia.

CD33 is variably expressed on leukemia blasts in almost all patients with acute myeloid leukemia (AML) and possibly leukemia stem cells in some. Efforts to target CD33 therapeutically have focused on gemtuzumab ozogamicin (GO; Mylotarg), an antibody-drug conjugate delivering a DNA-damaging calicheamicin derivative. GO is most effective in acute promyelocytic leukemia but induces remissions in other AML types and received accelerated approval in the US in 2000. However, because a large follow-up study showed no survival improvement and increased early deaths the drug manufacturer voluntarily withdrew the US New Drug Application in 2010. More recently, a meta-analysis of data from several trials reported better survival in adults with favorable- and intermediate-risk cytogenetics but not adverse-risk AML randomized to receive GO along with intensive induction chemotherapy. As a result, GO is being re-evaluated by regulatory agencies. Responses to GO are diverse and predictive biological response markers are needed. Besides cytogenetic risk, ATP-binding cassette transporter activity and possibly CD33 display on AML blasts may predict response, but established clinical assays and prospective validation are lacking. Single-nucleotide polymorphisms in CD33 may also be predictive, most notably rs12459419 where the minor T-allele leads to decreased display of full-length CD33 and preferential translation of a splice variant not recognized by GO. Data from retrospective analyses suggest only patients with the rs12459419 CC genotype may benefit from GO therapy but confirmation is needed. Most important may be markers for AML cell sensitivity to calicheamicin, which varies over 100 000-fold, but useful assays are unavailable. Novel CD33-targeted drugs may overcome some of GO's limitations but it is currently unknown whether such drugs will be more effective in patients benefitting from GO and/or improve outcomes in patients not benefitting from GO, and what the supportive care requirements will be to enable their safe use.

Genetic dissection for zinc deficiency tolerance in rice using bi-parental mapping and association analysis.

KEY MESSAGE: Zinc deficiency is a widespread soil constraint in rice production. Here, we present QTL/candidate genes associated with Zn deficiency tolerance identified through bi-parental QTL mapping and genome-wide association analysis. Zinc (Zn) deficiency is a widespread soil constraint in rice production. Despite several physiological studies elucidating Zn deficiency tolerance mechanisms, little is known about genetic factors conferring tolerance. To identify QTL associated with root development, biomass accumulation, and grain yield under Zn deficiency, we combined bi-parental QTL mapping in a population of 200 backcross inbred (BC1F6) lines and genome-wide association analysis using 247 k SNP markers across 140 accessions of an indica diversity panel. Three QTLs for Zn deficiency tolerance on chromosomes 3, 6, and 12 co-localized in both approaches and the association analysis detected two additional strong QTL on chromosomes 1 and 9 not present in the bi-parental population. Based on haplotype analysis of the indica panel, biomass consistently increased due to the minor 'tolerance' haplotypes, which had frequencies between 13 and 34%. By utilizing the previous transcript data collected from the same Zn-deficient field, we identified one putative candidate gene within the chromosome 6-QTL, which was associated with all traits in both analyses. Gene Os06g44220 was barely expressed under +Zn conditions but strongly upregulated in both root and shoot under stress and consistently more so in the tolerant genotype. Os06g44220 is an uncharacterized gene with expression previously detected only under salinity stress. Four SNP alterations within the promoter region distinguish the two alleles identified and a genotype tolerant to Zn deficiency shares the same allele as salinity tolerant varieties, lending support to the hypothesis that this gene may confer tolerance to both stresses.