ABSTRACT
We investigated the ability of surfactant-induced spreading to promote nanoparticle distribution on model mucus hydrogels. The hydrogels were formulated with viscoelastic properties and surface tensions that match those of native lung mucus. Nanoparticle-containing droplets with or without surfactant were deposited on the mucus surface and spreading patterns were monitored by time-course fluorescence imaging. Overall, surfactant-induced spreading of nanoparticles required an appropriate balance between Marangoni forces and viscoelastic subphase resistance. Spreading was enhanced on bare gels by increasing the concentration of surfactant in the droplets or reducing the viscoelastic properties of the subphase. However, with a pre-existing film of pulmonary surfactant on the mucus surface, spreading was dramatically inhibited as the surface tension gradient between the droplets and the surrounding subphase decreased. A complete lack of spreading was observed at surface tensions that matched those in the tracheobronchial region of the lungs, even with full-concentration Infasurf. These studies demonstrate that the magnitude of spreading on lung mucus-like surfaces is limited by native mucosal properties.
