Efeito do ômega 3 e da vitamina B12 no espermograma, na histomorfometria dos órgãos reprodutivos e nas temperaturas do corpo com termografia infravermelha em ratos Wistar / Effect of Omega 3 and Vitamin B12 on the spermogram, histomorphometry of the reproductive organs and body temperatures with infrared thermography in Wistar rats

Objetivou-se estudar o efeito do ômega 3 e da vitamina B12 no espermograma, na histomorfometria dos órgãos reprodutivos e na temperaturas do corpo com termografia infravermelha em ratos Wistar. Utilizaram-se 16 ratos, em quatro grupos (n=4), que receberam injeções diárias por 30 dias, sendo: grupo controle - solução salina; grupo ômega 3 - óleo de peixe 1g/kg; grupo B12 - vitamina B12 3µg; e grupo ômega 3 + B12 - óleo de peixe 1g/kg e vitamina B12 3µg. Imagens termográficas de áreas do corpo foram obtidas. No 30º dia, os ratos foram sacrificados e realizaram-se as análises de morfologia espermática e histomorfometria. Os dados foram submetidos à análise de variância e ao teste de Tukey a 5%. A temperatura da superfície do escroto foi superior no grupo B12 (P<0,05). Não houve diferenças entre grupos (P>0,05) para temperaturas do globo ocular. Houve correlação entre temperatura da superfície do escroto e porcentagem de gota citoplasmática distal (P=0,678). A elevação da temperatura do escroto resulta no aumento da porcentagem de gotas citoplasmáticas distais. A temperatura do globo ocular não sofre influência significativa do ômega 3 e da vitamina B12. O ômega 3 reduz o epitélio seminífero, e a vitamina B12 minimiza esse efeito.(AU)

The objective of this study was to study the effect of Omega 3 and vitamin B12 on spermogram, histomorphometry of reproductive organs and body temperature with infrared thermography in Wistar rats. Sixteen rats were used in four groups (n= 4) who received daily injections for 30 days. Control Group - saline solution; Group Omega 3 - fish oil 1g/kg; Group B12 - vitamin B12 3μg and Group Omega 3 + B12 - fish oil 1g/kg and vitamin B12 3μg. Thermographic images of body were obtained. On the 30th day the rats were sacrificed and analyzes of sperm morphology and histomorphometry were performed. Data were submitted to analysis of variance and Tukey's test at 5%. The surface temperature of the scrotum was higher in group B12 (P< 0.05). There were no differences between groups (P> 0.05) for eyeball temperatures. There was a correlation between scrotal temperature and distal cytoplasmic droplet (P= 0.678). Elevation of scrotum temperature results in an increase in the percentage of distal cytoplasmic droplets. The temperature of the eyeball is not significantly influenced by Omega 3 and vitamin B12. Omega 3 reduces the seminiferous epithelium and vitamin B12 minimizes this effect.(AU)

Concentração sérica de progesterona, pH da vagina e morfometria corpórea de cervas gestantes (Cervus unicolor) em cativeiro / Serum concentration of progesterone, pH of the vagina and body morphometryof pregnant deer (Cervus unicolor)in captivity

The aim was to study pregnant cervus in captivity, to obtain body morphometric data, serum progesterone concentrations, and pH of the vagina. Females of cervus (Cervus unicolor), (n=8) between 2 and 3 years old were used in November during breeding season. After sedation, in one collection, the following was measured: body weight 54.50 ± 18.70kg, body length 1.21 ± 0.16m, height at withers 0.75 ± 0.08m, thoracic perimeter 0.84 ± 0.12m, and body mass index 97.32 ± 10.50kg / m2. After sedation, blood samples were collected to measure progesterone concentration by radioimmunoassay and the use of an indicator tape to obtain the pH of the vagina was performed eight times at four day intervals in the eight females, in a total of 64 samples for each exam. Data were analyzed, and significance was at 5%. There were no differences (P> 0.05) between measurements for serum progesterone concentrations and pH of the vagina. The correlations between body weight, progesterone, pH, and body mass index were not significant (P> 0.05). There was no significant oscillation of progesterone concentration and pH of the vagina in the pregnancy period studied.(AU)

Concentração sérica de progesterona, pH da vagina e morfometria corpórea de cervas gestantes (Cervus unicolor) em cativeiro / Serum concentration of progesterone, pH of the vagina and body morphometryof pregnant deer (Cervus unicolor)in captivity

The aim was to study pregnant cervus in captivity, to obtain body morphometric data, serum progesterone concentrations, and pH of the vagina. Females of cervus (Cervus unicolor), (n=8) between 2 and 3 years old were used in November during breeding season. After sedation, in one collection, the following was measured: body weight 54.50 ± 18.70kg, body length 1.21 ± 0.16m, height at withers 0.75 ± 0.08m, thoracic perimeter 0.84 ± 0.12m, and body mass index 97.32 ± 10.50kg / m2. After sedation, blood samples were collected to measure progesterone concentration by radioimmunoassay and the use of an indicator tape to obtain the pH of the vagina was performed eight times at four day intervals in the eight females, in a total of 64 samples for each exam. Data were analyzed, and significance was at 5%. There were no differences (P> 0.05) between measurements for serum progesterone concentrations and pH of the vagina. The correlations between body weight, progesterone, pH, and body mass index were not significant (P> 0.05). There was no significant oscillation of progesterone concentration and pH of the vagina in the pregnancy period studied.

Concomitant Malignant Pulmonary Peripheral Nerve Sheath Tumour and Benign Cutaneous Peripheral Nerve Sheath Tumour in a Dog.

Peripheral nerve sheath tumours (PNSTs) are neoplastic growths derived from Schwann cells, perineural cells or both. Malignant PNSTs (MPNSTs) are uncommon in domestic animals. This report describes the concomitant occurrence of PNSTs in a 10-year-old female cocker spaniel with a clinical history of respiratory impairment. Grossly, there was a large infiltrative mass in the caudal lobe of the right lung; smaller nodules were observed in the other lobes of the right lung. Furthermore, a small encapsulated cutaneous nodule was observed on the left hindlimb. Histopathology of the pulmonary tumours revealed the proliferation of pleomorphic spindle-shaped cells with moderate mitotic index arranged in interwoven bundles and concentric Antoni A and Antoni B patterns; invasion of the adjacent pulmonary tissue was observed. The cutaneous nodule consisted of neoplastic mesenchymal cells in interwoven bundles with concentric whorls, but without the marked anisokaryosis, binucleation and infiltrative growth seen in the pulmonary tumour. Immunohistochemistry revealed that both tumours were immunoreactive for vimentin, glial fibrillary acidic protein and S100 protein, but were negative for factor VIII. These findings are indicative of a MPNST in the lung with a concomitant benign PNST in the limb. This case represents the first report of a primary MPNST in the lung of a dog. This neoplastic growth should be included in the differential diagnosis of primary malignant pulmonary tumours of dogs.