[[Technology Surgical Intervention in Ulcerative Colitis Modeling Descending Part of the Colon in Rats Line "Wistar"].]

BACKGROUND: Ulcerative colitis (UC) is a chronic immune inflammatory condition of the colon with an unknown aetiology, leading to disability and reduced quality of life of patients. UC primarily affects young adults. In most cases, inflammatory bowel disease (iBD) debuts at reproductive age. The incidence of UC and severe clinical course has increased overall across the world. The study of the mechanisms of pathogenesis and aetiology of this disease contributes to the development of new effective methods of treatment. OBJECTIVE: The aim of our study was to develop technology of the surgery directed to induction of reversible ischemic damage, the erosive-ulceration of gut mucosae (descending colon) at rats of the WISTAR line. MATERIALS AND METHODS: Experimental research was done using male rats < (n=40), their age was more than 6 months, weight of body is 280 - 350 grams. The animals were separated into 3 groups: 1 - (n=14) Sholimov's operative method, 2 group (n=16) - model using special technology, 3 group (n=10) - Coopr's H. S. induction ulcerative colitis. Observation was been done during 10 days. We offered special surgical technique: parietal ligation on every vasa recta of colon descendens of rats the length 3 cm along colon, on the apex vesicae and then vasa recta are cut. Then during 7 days 1% solution of dextran sulfate sodium (DSN) was given for experimental animals after postoperative period. By the histolog- ical biopsy of colon mucosa the results were estimated. RESULTS/CONCLUSION: we have created erosive-ulceration of gut mucosae (descending colon) of rat with productive inflam- mation, vasculitis and plasmatic impregnation of the vessel walls and intimal thickening. Our model of ulcerative colitis can be used for the development and testing of new methods for the study and treatment of this pathology.

[Gastroesophageal reflux disease: pathogenesis and morbid anatomy].

By analyzing the current data available in the literature, the authors consider the pathogenesis and morbid anatomy of gastroesophageal reflux disease. They assess the role of the anatomic and physiological features of the esophagogastric transition in the development of reflux-esophagitis and its complications. The principles of morphological diagnosis are considered in the interpretation of esophageal biopsy in reflux-esophagitis and Barrett's esophagus.

[Clinicomorphological diagnosis and surgical treatment of complicated gastroesophageal reflux disease].

Biopsy and surgery specimens taken from 224 patients with gastroesophageal reflux disease were clinically and morphologically analyzed. The morphological diagnosis of this condition is of more informative value than endoscopy and X-ray study. Malignization occurs in 25% of patients with Barrett oesophagus. One case of surgical treatment for gastroesophageal reflux disease yielding a good outcome is described.

[Wound healing process differences in type I and type II diabetes mellitus]. / Osobennosti techeniia ranevogo protsessa pri I i II tipe sakharnogo diabeta.

In 88 patients with purulent wounds in diabetes mellitus the course of wound healing was studied depending on the type of the disease. Clinical and morphological distinctions of the wound process in type 1 and type 2 diabetes mellitus were revealed.

[Analysis of the mechanism of the ulcerostatic effect of tris-[2-hydroxyethyl] ammonium salt of iron-containing polyacrylic acid]. / Analiz mekhanizma ul'tserostaticheskogo deistviia tris-[2-oksiétil] ammoniinoi soli zhelezosoderzhashchei poliakrilovoi kisloty.

It was demonstrated in experiments on rats with acetate gastric ulcer that ammonium salt tris-(2-hydroxyethyl) of iron-containing polyacrylic acid intensifies the development of granulation-fibrous tissue and promotes fuller restoration of connective-tissue polymers--collagen, noncollagenous proteins, and glycosaminoglycans in it. The biochemical results are confirmed by histological studies.

[The detection of albumin intraperitoneally administered to rabbits in the subclavian lymph node]. / Vyiavlenie intraperitoneal'no vvedennogo krolikam al'bumina v podkliuchichnom limfouzle.

With light and electron microscopy, the localization of human albumin labeled with colloidal gold is described in the subclavia lymph nodes of rabbits following an intraperitoneal injection of this labeled albumin. Most of the particles were found in the reticular cells of the sinus, and some particles were identified in the sinus macrophages. No particles were found inside lymph node follicules within 1 hour after injection. All stages of internalization of foreign protein inside lymph node cells were demonstrated.

[Diagnosis and surgical treatment of reflux esophagitis]. / Diagnostika i khirurgicheskoe lechenie refliuks-ézofagita.

Operations were performed on 192 patients with reflux esophagitis, 23 of them had peptic stricture of the esophagus. Esophago-fundoplication was the main operation. Nissen's (106), Tupe (47), Belsi's (3), Dor's (5), and atypical methods were applied. Whenever indicated it was supplemented by crurorhaphy, SPV, pylorotomy, correction of the duodenal junction, etc. Resection of the esophagus (19) was performed with one-stage esophagoplasty by means of the stomach through a left thoracoabdominal approach (14), the whole stomach passed through the posterior mediastinum from an abdomino-cervical approach (2) and the whole stomach with Lewis' intrathoracic anastomosis (3). Distal gastric resection was carried out in 6 and other operations in 3 patients. The mortality was 1%. Reoperations were performed in 5 patients. The results were good in 81.2% of cases. The tactics is individualized according to the presence or absence of a stricture, its length, and localization of the upper border.

[Effect of 1-(chloromethyl)silatrane on biochemical indices in the tissue of experimental gastric ulcer]. / Vliianie 1-(khlormetil)silatrana na biokhimicheskie pokazateli tkani éksperimental'noi iazvy zheludka.

It has been demonstrated in rats with experimental gastric ulcer that 1-(chloromethyl) silatrane intensifies the development of granulation-fibrous tissue and favours optimization of the ratio of collagens and glycosaminoglycans, the main connective tissue biopolymers. Furthermore, 1-(chloromethyl) silatrane produces a more pronounced effect on hexosamine-containing glycoproteins in the ulcer tissue as compared with methyluracil and oxyferriscorbon. At the same time, judging by the time course of sialoproteins, the antiulcerous drugs, methyluracil and oxyferriscorbon, are much more effective antiinflammatory agents than 1-(chloromethyl)silatrane.

[Clinical variants of chronic ischemic syndrome of the lower extremities]. / Klinicheskoe varianty khronicheskogo ishemicheskogo sindroma nizhnikh konechnostei.

Analysis is made of 1,014 cases with occlusive lesion of the bifurcation of the aorta and iliofemoral segment in patients with chronic ischemic syndrome of the lower limbs. The cause of high arterial occlusion with atherosclerosis in 95.5% of patients and nonspecific aortitis in 12 cases. A group of 20 patients with selective lesion of the femoral segment, which is distinguished from atherosclerosis obliterans, endarteritis with ascending thrombosis, and nonspecific aortitis by its clinico-angiographic and morphological manifestations, was analyzed in detail. This form of arterial occlusion is called "segmental thromboarteritis". The results of reconstructive operations in most patients with "segmental thromboarteritis" proved unfavourable.