ABSTRACT
The authors present the results of immunization of horses-producers with a commercial antigen and the yolk culture of the living R. sibericus (strain K1) for the purpose of obtaining specific immune sera for many purposes. It was shown that the original combined scheme of immunization and reimmunization of horses, successfully approved in the preparation of immune sera to Rickettsia prowazeki also proved to be highly effective for obtaining the antisera to R. sibericus. Sera obtained after the primary immunization of horses could be successfully used as diagnostic sera, but they were of no use for the preparation of fluorescent antibodies and the highly active hemosensitin; as to the sera obtained after the remote reimmunization--they were universal raw material. Dry fluorescent antibodies to R. sibericus were prepared of the horse sera (their stain titre was 1 : 256--1 : 512) a highly active hemosensitin, and also dry commercial diagnostic sera for the IHT, CFR and the immunofluorescent reaction of microagglutination.
Subject(s)
Horses/immunology , Immunization , Rickettsia/immunology , Rickettsial Vaccines , Vaccines , Agglutination Tests , Animals , Antibodies, Bacterial/analysis , Antibody Formation , Complement Fixation Tests , Drug Evaluation, Preclinical , Fluorescent Antibody Technique , Hemagglutination Tests , Hemolysis , Immunization, Secondary , Male , Mice , Time FactorsABSTRACT
A dry polyvalent erythrocyte immunoglobulin diagnostic preparation for arboviruses of Group B may be prepared to be used in the indirect hemagglutination test. The preparation detects antigens of arboviruses of this group in the tissue culture fluid and in suckling mouse brain tissue treated with sucrose-acetone. The test with Group B arbovirus antigens is inhibited specifically by the homologous polyvalent immune gamma globulin. Inhibition of hemagglutination by species-specific immune globulins to individual members of Group B arboviruses permits typing of tick-borne encephalitis birus only. In making the erythrocyte diagnostic preparation, the optimal concentration of the sensitizing immune gamma globulin should be determined experimentally.
