Subject(s)
Bacterial Proteins/genetics , Chromosomes, Bacterial/genetics , Escherichia coli Proteins , Mycoplasma/genetics , Operon , Ribosomal Proteins/genetics , Amino Acid Sequence , Aminopeptidases/genetics , Base Sequence , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , L-Lactate Dehydrogenase/chemistry , L-Lactate Dehydrogenase/genetics , Methionyl Aminopeptidases , Molecular Sequence Data , Mycoplasma/enzymology , Physical Chromosome Mapping , SEC Translocation Channels , Sequence Homology, Amino AcidABSTRACT
The genomic library of Mycoplasma gallisepticum was constructed and two clones, selected by hybridization on E. coli 16S rRNA, were analyzed. The restriction map of the clones indicate that both clones belong to the same region of the M. gallisepticum genome. The results of Southern hybridization with either E. coli 16S rRNA, or E. coli 23S rRNA, or oligonucleotide synthesized as a part of M. gallisepticum 5S rRNA, led to the conclusion that the unspliced rRNA operon was cloned. The order of genes in the operon is common for eubacteria: 16S-23S-5S. The tuf gene of M. gallisepticum was mapped inside the cloned region 5 kb upstream of 16S gene by hydridization on E. coli tufA gene and oligonucleotide synthesized on the basis of M. gallisepticum tuf gene sequence. The direction of transcription of the gene and the expected direction of transcription of the rRNA operon coincide.
