Subject(s)
Chemokine CXCL12/metabolism , Granulocyte Colony-Stimulating Factor/pharmacology , Hematopoietic Stem Cell Mobilization , Hematopoietic Stem Cells/cytology , MicroRNAs/genetics , Signal Transduction , Animals , Hematopoietic Stem Cells/metabolism , Mice , Mice, Knockout , Polymorphism, Single NucleotideABSTRACT
The harmfulness of mealybugs resulting from sucking plant sap, secreting honeydew, and transmitting plant viruses can give them the status of serious pests. This study documents the influence of Pseudococcus maritimus (Ehrhorn) and Pseudococcus longispinus (Targioni Tozzetti) infestation on alterations in selected physiological parameters of Phalaenopsis x hybridum 'Innocence'. The condition of the cytoplasmic membranes was expressed as the value of thiobarbituric acid reactive substances. We have determined changes in the activities of catalase and guaiacol peroxidase and measured the following chlorophyll fluorescence parameters: maximum quantum yield of photosystem II (Fv/Fm), effective quantum yield (Y), photochemical quenching (qP), and nonphotochemical quenching (qN). The strongest physiological response of orchids was recorded in the initial period of mealybugs infestation. Prolonged insect feeding suppressed lipid peroxidation, peroxidase and catalase activity, as well as photosynthesis photochemistry. The pattern of changes was dependent on mealybug species. This indicated the complexity of the processes responsible for plant tolerance. Data generated in this study have provided a better understanding of the impact of two mealybug species infestation on Phalaenopsis and should be useful in developing pest management strategies.
Subject(s)
Antibiosis , Hemiptera/physiology , Herbivory , Orchidaceae/physiology , Animals , Female , NymphABSTRACT
Cultivated orchids are the most abundantly attacked by polyphagous mealybugs. This study documented how different density of mealybug Pseudococcus longispinus (Targioni Tozzetti) infestation is associated with a response of antioxidative systems of Phalaenopsis × hybridum 'Innocence'. The degree of cell damage, estimated by electrolyte leakage measurement and the level of thiobarbituric acid reactive substances (TBARS), the content of pigments as well as the activity of antioxidative enzymes and proline level, as measurements of stress and stress compensation in moth orchid were examined. The highest electrolyte leakage (E L) value among samples from colonized plants was found in the orchids from series III (50 individuals/plant), whereas the lowest in the plants from series II (20 individuals/plant). The TBARS content reached the highest level at the lowest number of feeding insects (series I). Peroxidase activity toward guaiacol was significantly increased in series I (5 individuals/plant). The highest catalase activity was recorded in plants colonized by the highest number of scale insects (series III). Whereas, the highest value of proline was in series II. The content of individual photosynthetic pigments (chlorophyll a, chlorophyll b and carotenoids) in plant tissues did not vary significantly between control and colonized orchids. The results have not confirmed hypothesis that the increasing number of mealybugs occurring on plant enhanced plant physiological response. The degree of longtailed mealybug infestation on plants was positively correlated only with electrolyte leakage and catalase activity in leaf tissues.
Subject(s)
Antioxidants/metabolism , Hemiptera/physiology , Hybridization, Genetic/genetics , Orchidaceae/genetics , Orchidaceae/metabolism , Orchidaceae/parasitology , Plant Leaves/metabolism , Analysis of Variance , Animals , Carotenoids/metabolism , Catalase/metabolism , Cell Membrane/metabolism , Chlorophyll/metabolism , Chlorophyll A , Electrolytes/metabolism , Peroxidase/metabolism , Population Density , Proline/metabolism , Thiobarbituric Acid Reactive SubstancesABSTRACT
The role of corticosterone (Cort), the immune system's major stress hormone, in the regulation of hematopoietic stem and progenitor cells (HSPCs) and their dynamic bone marrow (BM) microenvironment is currently unknown. We report that corticotropin-releasing factor receptor 1 (CRFR1) mutant mice with chronically low Cort levels showed aberrant HSPC regulation, having higher HSPC numbers and upregulation of the chemokine CXCL12, phenotypes that were restored by Cort supplementation. Expanded stromal progenitors known to support HSPCs were also observed in these low-Cort-containing mice. A similar phenotype was induced in wild-type (WT) mice by Metyrapone, a Cort synthesis inhibitor. Conversely, high Cort exposure induced HSPC apoptosis, reduced long-term BM repopulation and decreased stromal progenitor cell numbers. We documented circadian oscillations of Cort in WT BM but not in CRFR1 mutant mice, leading to diminished circadian BM CXCL12 fluctuations and increased number of circulating HSPCs in these mice. Finally, low Cort induced expansion of stromal progenitors, CXCL12 expression, HSPC proliferation and BM repopulation capacity, involving Notch1 signaling. This was associated with upregulation of the Notch ligand, Jagged1, in BM myeloid cells. Our results suggest that daily physiologic Cort oscillations are critical for balanced HSPC proliferation and function involving Notch1 signaling and their supportive BM microenvironment.
