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1.
Bioorg Med Chem ; 17(6): 2428-32, 2009 Mar 15.
Article in English | MEDLINE | ID: mdl-19243952

ABSTRACT

Bulged sites in DNA and RNA have become targets for rational drug design due to their suspected involvement in a number of key biomolecular processes. A lead compound, derived from the enediyne natural product NCS-chrom has been used to inform chemical synthesis of a family of designed probes of DNA bulges, one of which shows 80 nM affinity for a two base bulged target. Key contributors to binding of these spirocyclic compounds have been studied in order to correlate affinity and specificity with structural features. Herein, we demonstrate that the glycosyl linkage stereochemistry of the pendant aminofucosyl group plays a pivotal role in binding, and coupled with insight obtained with various bulged targets, will allow rational design of second generation ligands.


Subject(s)
DNA Probes , Zinostatin/chemistry , Base Sequence , Fluorescence , Magnetic Resonance Spectroscopy , Spectrometry, Mass, Electrospray Ionization , Stereoisomerism
2.
Curr Top Med Chem ; 8(6): 436-47, 2008.
Article in English | MEDLINE | ID: mdl-18397167

ABSTRACT

Of the commonly recognized structural elements within nucleic acids, bulges are among the least developed as targets for small molecules. Bulges in DNA and RNA have been linked to biomolecular processes involved in numerous diseases, thus probes with affinity for these targets would be of considerable utility to chemical biologists and medicinal chemists. Despite such opportunity, there is a dearth of small molecules available with affinity for bulges, which has hampered exploitation of these key targets. We have used guided chemical synthesis to prepare small molecules capable of binding to DNA and RNA bulges. Our design is based on a template which mimics a metabolite of the enediyne neocarzinostatin. The key spirocylic building block was formed through an intramolecular aldol process and the parent template shows pronounced affinity for 2 base bulges. Functionalization with specific aminosugar moieties confers nanomolar binding affinity for selected bulged DNA targets, and installation of reactive functional groups allows covalent modification of bulges. These rationally designed agents can now be used to study the stereochemistry and architecture of bulge-drug complexes and investigate the molecular biology of bulge induced processes. Members of this class have been shown to induce slipped synthesis of DNA, suggesting the agents, in addition to recognizing and binding to pre-formed bulges, can also induce bulge formation on demand.


Subject(s)
DNA/chemistry , Enediynes/chemistry , Base Sequence , Drug Design , Enediynes/chemical synthesis , Models, Biological , Molecular Probes/chemical synthesis , Molecular Probes/chemistry , Nucleic Acid Conformation , Nucleic Acid Heteroduplexes/chemistry , Oligonucleotides/chemistry , Zinostatin/analogs & derivatives , Zinostatin/chemical synthesis , Zinostatin/chemistry
3.
Biochemistry ; 46(16): 4793-803, 2007 Apr 24.
Article in English | MEDLINE | ID: mdl-17388570

ABSTRACT

The solution structure of the complex formed between an oligodeoxynucleotide containing a two-base bulge (5'-CCATCGTCTACCTTTGGTAGGATGG) and SCA-alpha2, a designed spirocyclic helical molecule, has been elucidated. SCA-alpha2, a close mimic of the metabolite, NCSi-gb, of the DNA bulge-specific enediyne antibiotic neocarzinostatin, differs in possessing a more stable spirocyclic ring system and in lacking certain bulky groupings that compromise bulged DNA binding. This study provides a detailed comparison of the binding modes of the two complexes and provides new insights into the importance of shape and space, as opposed to simple nucleotide sequence, in complex formation at the bulge site. The two rigidly held aromatic rings of SCA-alpha2 form a right-handed helical molecular wedge that specifically penetrates the bulge-binding pocket and immobilizes the two bulge residues (GT), which point toward the minor groove, rather than the major groove as in the NCSi-gb.bulged DNA complex. The ligand aromatic ring systems stack on the DNA bulge-flanking base pairs that define the long sides of the triangular prism binding pocket. Like NCSi-gb, SCA-alpha2 possesses the natural N-methylfuranose moiety, alpha-linked to the benzindanol (BI) moiety. The amino sugar anchors in the major groove of the DNA and points toward the 3'-bulge-flanking base pair. Lacking the bulky cyclocarbonate of NCSi-gb, the SCA-alpha2.bulged DNA complex has a much less twisted and buckled 3'-bulge-flanking base pair (dG20.dC8), and the G20 residue stacks directly above the BI ring platform. Also, the absence of the methyl group and the free rotation of the methoxy group on the dihydronaphthanone (NA) moiety of SCA-alpha2 allow better stacking geometry of the NA ring above the 5'-bulge-flanking dG21.dC5 base pair. These and other considerations help to explain why NCSi-gb binds very poorly to bulged RNA and are consistent with the recent observation of good binding with SCA-alpha2. Thus, although the two complexes resemble each other closely, they differ in important local environmental details. SCA-alpha2 has a better hand-in-glove fit at the bulge site, making it an ideal platform for the placement of moieties that can react covalently with the DNA and for generating congeners specific for bulges in RNA.


Subject(s)
DNA/chemistry , Enediynes/chemistry , Fucose/analogs & derivatives , Spiro Compounds/chemistry , Fucose/chemistry , Ligands , Models, Molecular , Nuclear Magnetic Resonance, Biomolecular , Zinostatin/analogs & derivatives , Zinostatin/chemistry
4.
Biochemistry ; 46(2): 561-7, 2007 Jan 16.
Article in English | MEDLINE | ID: mdl-17209566

ABSTRACT

Because bulged structures (unpaired bases) in nucleic acids are of general biological significance, it has been of interest to design small molecules as specific probes of bulge function. On the basis of our earlier work with the specific DNA bulge-binding metabolite obtained from the enediyne antitumor antibiotic neocarzinostatin chromophore (NCS-chrom), we have prepared three small helical spirocyclic molecules that most closely mimic the natural product. These wedge-shaped molecules resemble the natural product in having the sugar residue attached to the same five-membered ring system. In one instance, the sugar is aminoglucose in beta-glycosidic linkage, and in the other, two enantiomers have the natural sugar N-methylfucosamine in alpha-glycosidic linkage. All three analogues were found to interfere with bulge-specific cleavage by NCS-chrom and the ability of bulged DNA to serve as a template for DNA polymerase 1 in accordance with their binding affinities for DNA containing a two-base bulge. Comparable results were obtained with the analogues for the less efficiently cleaved three-base bulge DNA structures. In each situation, the enantiomers possessing the natural sugar in alpha-glycosidic linkage are the most potent inhibitors of the cleavage reaction. In the DNA polymerase reactions, again, the closest natural product mimics were the most effective in selectively impeding nucleotide extension at the bulge site, presumably by complex formation. These results demonstrate the potential usefulness of bulge-binding compounds in modifying DNA structure and function and support efforts to design and prepare reactive species of these molecules that can covalently modify bulged DNA.


Subject(s)
DNA/chemistry , Molecular Probes/chemistry , Spiro Compounds/chemistry , Base Sequence , DNA Primers/chemistry , Drug Design , Molecular Probes/chemical synthesis , Molecular Structure , Nucleic Acid Conformation , Spiro Compounds/chemical synthesis
5.
Bioorg Med Chem ; 15(2): 784-90, 2007 Jan 15.
Article in English | MEDLINE | ID: mdl-17085052

ABSTRACT

Bulged structures in DNA and RNA have been linked to biomolecular processes involved in numerous diseases, thus probes with affinity for these nucleic acid targets would be of considerable utility to chemical biologists. Herein, we report guided chemical synthesis of small molecules capable of binding to DNA bulges by virtue of their unique (spirocyclic) geometry. The agents, modeled on a natural product congener, show pronounced selectivity for specific bulged motifs and are able to enhance slipped DNA synthesis, a hallmark functional assay of bulge binding. Significantly, bulge-agent complexes demonstrate characteristic fluorescent signatures depending on bulge and flanking sequence in the oligo. It is anticipated that these signature patterns can be harnessed as molecular probes of bulged hotspots in DNA and RNA.


Subject(s)
DNA/chemistry , Indicators and Reagents , Magnetic Resonance Spectroscopy , Molecular Probes , Nucleic Acid Conformation , Oligonucleotide Probes , Spectrometry, Mass, Electrospray Ionization
6.
Chem Commun (Camb) ; (42): 4431-3, 2006 Nov 13.
Article in English | MEDLINE | ID: mdl-17057867

ABSTRACT

Based on fluorescence binding studies and 1D 1H NMR studies, designed synthetic analogues of NCSi-gb bind specifically with two-base bulged RNA, including HIV-2 TAR RNA, making them potential lead compounds for antiviral drug development.


Subject(s)
Fucose/analogs & derivatives , HIV Long Terminal Repeat , HIV-2/genetics , RNA, Viral/metabolism , Spiro Compounds/chemistry , Fucose/chemistry , Fucose/metabolism , Humans , Models, Molecular , Molecular Structure , RNA, Viral/chemistry , Spiro Compounds/metabolism
7.
Bioorg Med Chem Lett ; 16(11): 2895-9, 2006 Jun 01.
Article in English | MEDLINE | ID: mdl-16546380

ABSTRACT

NCSi-gb is a neocarzinostatin chromophore (NCS-chrom) metabolite which binds strongly to certain two-base DNA bulges. Compared with previously reported NCSi-gb analogues, a new analogue with a different aminoglycoside position was synthesized, and it showed strong fluorescence and improved binding and sequence selectivity to DNA bulges. The N-dimethylated form of this analogue had a similar binding pattern, and it competitively inhibited bulge-specific cleavage by NCS-chrom.


Subject(s)
DNA/chemistry , Molecular Probes/chemistry , Circular Dichroism , Molecular Structure
8.
Org Lett ; 7(1): 71-4, 2005 Jan 06.
Article in English | MEDLINE | ID: mdl-15624980

ABSTRACT

A designed molecule with capacity to bind DNA bulges (20) has been prepared from readily available starting materials. The spirocyclic template was modeled on a metabolite of neocarzinostatin chromophore (NCSi-gb) and is equipped with functionality to enable convenient bioassay. Preliminary studies confirm binding at specific bulged sequences and induction of polymerase-mediated slippage events. The target compound offers a convenient means to study affinity for unique bulged motifs and for use as a molecular biology reagent.


Subject(s)
DNA/chemistry , Models, Molecular , Nucleic Acid Conformation
9.
Org Lett ; 6(26): 4833-6, 2004 Dec 23.
Article in English | MEDLINE | ID: mdl-15606078

ABSTRACT

[reaction: see text] Synthesis of chiral spirocyclic helical compounds which mimic the molecular architecture of the potent DNA bulge binder obtained from the antitumor agent NCS-chrom has been accomplished. Structural analysis of the compounds by CD and NMR is presented. NMR titration study indicates binding of P,alpha-helimer (1d) at a two-base bulge site in a DNA oligomer, providing insight to the design of agents as specific probes of a bulged structure in nucleic acids.


Subject(s)
DNA/chemistry , Magnetic Resonance Spectroscopy/methods , Spiro Compounds/chemical synthesis , Cyclization , Models, Molecular , Molecular Conformation , Oligonucleotides/chemistry , Spiro Compounds/chemistry , Stereoisomerism
10.
Bioorg Med Chem Lett ; 14(12): 3337-9, 2004 Jun 21.
Article in English | MEDLINE | ID: mdl-15149702

ABSTRACT

A designed molecule with capacity to alkylate DNA bulges has been prepared from readily available starting materials. The spirocyclic template utilized was designed on the basis of established architectures, and equipped with a mustard alkylating group. Preliminary studies confirm alkylation of specific bulged sequences, paving the way for second generation substrates with higher affinity.


Subject(s)
Alkylating Agents/chemical synthesis , DNA/genetics , DNA/metabolism , Nucleic Acid Conformation , Alkylating Agents/metabolism , Base Sequence/physiology , Binding Sites/physiology
11.
Biochemistry ; 43(3): 641-50, 2004 Jan 27.
Article in English | MEDLINE | ID: mdl-14730968

ABSTRACT

The solution structure of the complex formed between an oligonucleotide containing a two-base bulge (5'-CACGCAGTTCGGAC.5'-GTCCGATGCGTG) and ent-DDI, a designed synthetic agent, has been elucidated using high-resolution NMR spectroscopy and restrained molecular dynamic simulation. Ent-DDI is a left-handed wedge-shaped spirocyclic molecule whose aglycone portion is an enantiomer of DDI, which mimics the spirocyclic geometry of the natural product, NCSi-gb, formed by base-catalyzed activation of the enediyne antibiotic neocarzinostatin. The benzindanone moiety of ent-DDI intercalates between the A6.T21 and the T9.A20 base pairs, overlapping with portions of the purine bases; the dihydronaphthalenone moiety is positioned in the minor groove along the G7-T8-T9 bulge sequence; and the aminoglycoside is in the middle of the minor groove, approaching A20 of the nonbulged strand. This alignment of ent-DDI along the DNA helical duplex is in the reverse direction to that of DDI. The aminoglycoside moiety of ent-DDI is positioned in the 3' direction from the bulge region, whereas that of the DDI is positioned in the 5' direction from the same site. This reverse binding orientation within the bulge site is the natural consequence of the opposite handedness imposed by the spirocyclic ring junction and permits the aromatic ring systems of the two spirocyclic enantiomers access to the bulge region. NMR and CD data indicate that the DNA in the DDI-bulged DNA complex undergoes a larger conformational change upon complex formation in comparison to the ent-DDI-bulged DNA, explaining the different binding affinities of the two drugs to the bulged DNA. In addition, there are different placements of the bulge bases in the helical duplex in the two complexes. One bulge base (G7) stacks inside the helix, and the other one (T8) is extrahelical in the DDI-bulged DNA complex, whereas both bulge bases in the ent-DDI-bulged DNA complex prefer extrahelical positions for drug binding. Elucidation of the detailed binding characteristics of the synthetic spirocyclic enantiomers provides a rational basis for the design of stereochemically controlled drugs for bulge binding sites.


Subject(s)
DNA/chemistry , Intercalating Agents/chemistry , Nucleic Acid Conformation , Binding Sites , Circular Dichroism , Crystallography, X-Ray , Enediynes , Nuclear Magnetic Resonance, Biomolecular , Nucleic Acid Heteroduplexes/chemistry , Oligonucleotides/chemistry , Stereoisomerism , Zinostatin/analogs & derivatives , Zinostatin/chemistry
12.
Biochemistry ; 42(28): 8472-83, 2003 Jul 22.
Article in English | MEDLINE | ID: mdl-12859193

ABSTRACT

The solution structure of the complex formed between an oligonucleotide containing a two-base bulge (5'-CACGCAGTTCGGAC.5'-GTCCGATGCGTG) and DDI, a designed synthetic agent, has been elucidated using high-resolution NMR spectroscopy and restrained molecular dynamic simulation. DDI, which has been found to modulate DNA strand slippage synthesis by DNA polymerase I [Kappen, L. S., Xi, Z., Jones, G. B., and Goldberg, I. H. (2003) Biochemistry 42, 2166-2173], is a wedge-shaped spirocyclic molecule whose aglycone structure closely resembles that of the natural product, NCSi-gb, which strongly binds to an oligonucleotide containing a two-base bulge. Changes in chemical shifts of the DNA upon complex formation and intermolecular NOEs between DDI and the bulged DNA duplex indicate that agent specifically binds to the bulge site of DNA. The benzindanone moiety of DDI intercalates via the minor groove into the G7-T8-T9.A20 pocket, which consists of a helical base pair and two unpaired bulge bases, stacking with the G7 and A20 bases. On the other hand, the dihydronaphthalenone and aminoglycoside moieties are positioned in the minor groove. The aminoglycoside, which is attached to spirocyclic ring, aligns along the A20T21G22 sequence of the nonbulged strand, while the dihydronaphthalenone, which is restrained by the spirocyclic structure, is positioned near the G7-T8-T9 bulge site. The aminoglycoside is closely aligned with the dihydronaphthalenone, preventing its intercalation into the bulge site. In the complex, the unpaired purine (G7) is intrahelical and stacks with the intercalating moiety of DDI, whereas the unpaired pyrimidine (T8) is extrahelical. The structure of the complex formed by binding of the synthetic agent to the two-base bulged DNA reveals a binding mode that differs in important details from that of the natural product, explaining the different binding specificity for the bulge sites of DNA. The structure of the DDI-bulged DNA complex provides insight into the structure-binding affinity relationship, providing a rational basis for the design of specific, high-affinity probes of the role of bulged nucleic acid structures in various biological processes.


Subject(s)
DNA/chemistry , Oligodeoxyribonucleotides/chemistry , Base Sequence , Binding Sites , Deoxyribonuclease I/metabolism , Kinetics , Magnetic Resonance Spectroscopy , Models, Molecular , Nucleic Acid Conformation , Solutions
13.
Biochemistry ; 42(5): 1186-98, 2003 Feb 11.
Article in English | MEDLINE | ID: mdl-12564921

ABSTRACT

Neocarzinostatin (NCS-chrom), a natural enediyne antitumor antibiotic, undergoes either thiol-dependent or thiol-independent activation, resulting in distinctly different DNA cleavage patterns. Structures of two different post-activated NCS-chrom complexes with DNA have been reported, revealing strikingly different binding modes that can be directly related to the specificity of DNA chain cleavage caused by NCS-chrom. The third structure described herein is based on recent studies demonstrating that glutathione (GSH) activated NCS-chrom efficiently cleaves DNA at specific single-base sites in sequences containing a putative single-base bulge. In this structure, the GSH post-activated NCS-chrom (NCSi-glu) binds to a decamer DNA, d(GCCAGAGAGC), from the minor groove. This binding triggers a conformational switch in DNA from a loose duplex in the free form to a single-strand, tightly folded hairpin containing a bulge adenosine embedded between a three base pair stem. The naphthoate aromatic moiety of NCSi-glu intercalates into a GG step flanked by the bulge site, and its substituent groups, the 2-N-methylfucosamine carbohydrate ring and the tetrahydroindacene, form a complementary minor groove binding surface, mostly interacting with the GCC strand in the duplex stem of DNA. The bulge site is stabilized by the interactions involving NCSi-glu naphthoate and GSH tripeptide. The positioning of NCSi-glu is such that only single-chain cleavage via hydrogen abstraction at the 5'-position of the third base C (which is opposite to the putative bulge base) in GCC is possible, explaining the observed single-base cleavage specificity. The reported structure of the NCSi-glu-bulge DNA complex reveals a third binding mode of the antibiotic and represents a new family of minor groove bulge DNA recognition structures. We predict analogue structures of NCSi-R (R = glu or other substituent groups) may be versatile probes for detecting the existence of various structures of nucleic acids. The NMR structure of this complex, in combination with the previously reported NCSi-gb-bulge DNA complex, offers models for specific recognition of DNA bulges of various sizes through binding to either the minor or the major groove and for single-chain cleavage of bulge DNA sequences.


Subject(s)
DNA/chemistry , Nucleic Acid Conformation , Zinostatin/chemistry , Antibiotics, Antineoplastic/chemistry , Base Composition , Binding Sites , DNA Adducts/chemistry , DNA Damage , DNA, Single-Stranded/chemistry , Enediynes , Macromolecular Substances , Molecular Conformation , Nuclear Magnetic Resonance, Biomolecular , Transformation, Genetic , Zinostatin/analogs & derivatives
14.
Biochemistry ; 42(7): 2166-73, 2003 Feb 25.
Article in English | MEDLINE | ID: mdl-12590606

ABSTRACT

It has been postulated that bulged structures may be intermediates in the DNA strand slippage synthesis associated with the expansion of nucleotide repeats in various neurodegenerative diseases and cancer. To probe the possible role of bulged structures in this process, we have synthesized a wedge-shaped spirocyclic molecule, DDI (double-decker intercalator), on the basis of our earlier work with the bulge-specific derivative prepared from the enediyne antitumor antibiotic neocarzinostatin chromophore. Using a series of primers/templates containing nucleotide repeats [(AAT)(3)/(ATT)(5), (ATT)(3)/(AAT)(5), (CAG)(3)/(CTG)(5), (CA)(4)C/(GT)(7)G, (GT)(4)G/(CA)(7)C, T(9)/A(30), T(20)/A(30)] with the Klenow fragment of Escherichia coli DNA polymerase I, we find that DDI markedly enhances the formation of long DNA products, whose synthesis would require strand slippage to occur. DDI-induced slippage synthesis is more pronounced as the incubation proceeds and at limiting enzyme levels. The gel band pattern of the synthesized DNA products reflects the particular nucleotide repeat unit and is not altered by DDI. The lack of any drug effect on primer extension on M13 DNA and heteropolymeric 62-mer templates, where strand slippage is much less likely to occur, suggests that stimulation of slippage synthesis by DDI is not due to a direct effect on the enzyme. By contrast, other DNA-binding agents, such as ethidium bromide, distamycin, and doxorubicin, inhibit the formation of slippage-induced DNA products, but this block can be overcome by DDI, presumably by its destabilizing duplex DNA-binding sites for these other agents. We propose that DDI binds to or induces the formation of a bulge or related structure, which promotes DNA strand slippage and its consequent expansion of nucleotide repeats during replication by DNA polymerase I and that this action provides insight into the development of agents that interfere with nucleotide expansions found in various disease states.


Subject(s)
DNA/biosynthesis , Intercalating Agents/chemistry , Nucleic Acid Conformation , Repetitive Sequences, Nucleic Acid , Spiro Compounds/chemistry , Bacteriophage M13/chemistry , Bacteriophage M13/genetics , Binding Sites , DNA/chemistry , DNA Polymerase I/chemistry , DNA, Single-Stranded/biosynthesis , DNA, Viral/chemistry , Ethidium/chemistry , Nucleic Acid Synthesis Inhibitors/chemistry , Oligodeoxyribonucleotides/chemistry , Templates, Genetic , Trinucleotide Repeats
15.
Chem Biol ; 9(8): 925-31, 2002 Aug.
Article in English | MEDLINE | ID: mdl-12204692

ABSTRACT

Bulged regions of nucleic acids are important structural motifs whose function has been linked to a number of key nuclear processes. Additionally, bulged intermediates have been implicated in the etiology of several genetic diseases and as targets for viral regulation. Despite these obvious ramifications, few molecules are capable of selective binding to bulged sequences. Prompted by the remarkable affinity of a natural product metabolite, we have designed and prepared a series of readily accessible synthetic agents with selective bulge binding activity. Furthermore, by screening a library of bulge-containing oligodeoxynucelotides, correlations between structure and affinity of the agents can be drawn. In addition to potential applications in molecular biology, the availability of these spirocyclic agents now opens the door for rational drug design.


Subject(s)
DNA/drug effects , Drug Design , Antineoplastic Agents/chemistry , Cyclization , DNA/chemistry , DNA/metabolism , Drug Evaluation, Preclinical , Enediynes , Gene Library , Hydrolysis/drug effects , Ligands , Nucleic Acid Conformation/drug effects , Oligodeoxyribonucleotides/chemistry , Oligodeoxyribonucleotides/metabolism , Spiro Compounds/chemical synthesis , Spiro Compounds/pharmacology , Structure-Activity Relationship , Zinostatin/analogs & derivatives , Zinostatin/chemistry
16.
Biochemistry ; 41(16): 5131-43, 2002 Apr 23.
Article in English | MEDLINE | ID: mdl-11955061

ABSTRACT

Our previous structure elucidation of the complexes of DNA and postactivated neocarzinostatin chromophore (NCS-chrom) compounds revealed two distinctly different binding modes of this antitumor molecule. A thorough understanding of these results will provide the molecular basis for the binding and DNA chain cleavage properties of NCS-chrom. NCSi-gb is one of the postactivated mimics of NCS-chrom which is formed under thiol-free conditions and is able to bind to DNA. This report describes the structure refinement of the NCSi-gb-bulge-DNA complex [Stassinopoulos, A., Jie, J., Gao, X., and Goldberg, I. H. (1996) Science 272, 1943-1946] and the NMR characterization of the free bulge-DNA and free NCSi-gb. These results reveal that the formation of the complex involves conformational changes in both the DNA and the ligand molecule. Of mechanistic importance for the NCS-chrom-DNA interaction, the two ring systems of the drug are brought closer to each other in the complex. This conformation correlates well with the previously observed marked enhancement of the formation of a DNA bulge cleaving species in the presence of bulge-DNA sequences, due to the promotion of the intramolecular radical quenching of the activated NCS-chrom. Interestingly, the binding of NCSi-gb promotes the formation of a bulge binding pocket; this was not found in the unbound DNA. NCS-chrom is unique among the enediyne antibiotics in its ability to undergo two different mechanisms of activation to form two different DNA binding and cleaving species. The two corresponding DNA complexes are compared. One, the bulge-DNA binder NCSi-gb, involves the major groove, and the second, the duplex binder NCSi-glu which is generated by glutathione-induced activation, involves the minor groove. Since the two NCS-chrom-related ligand molecules contain some common chemical structural elements, such as the carbohydrate ring, the striking differences in their DNA recognition and chain cleavage specificity provide insights into the fundamental principles of DNA recognition and ligand design.


Subject(s)
Antibiotics, Antineoplastic/chemistry , DNA, Single-Stranded/chemistry , Nucleic Acid Conformation/drug effects , Zinostatin/chemistry , Antibiotics, Antineoplastic/metabolism , Base Sequence , Binding Sites , Computer Simulation , DNA, Single-Stranded/metabolism , Enediynes , Ligands , Macromolecular Substances , Models, Molecular , Nuclear Magnetic Resonance, Biomolecular/methods , Sulfhydryl Reagents/chemistry , Zinostatin/analogs & derivatives , Zinostatin/metabolism
17.
Bioorg Med Chem ; 10(5): 1329-35, 2002 May.
Article in English | MEDLINE | ID: mdl-11886796

ABSTRACT

The binding of the wedge-shaped isostructural analogue of the biradical species of the chromphore of antitumor antibiotic neocarzinostatin to sequence-specific bulged DNAs results in alterations in ellipticity of the DNAs. Circular dichroism (CD) spectroscopic results suggest that the drug specifically recognizes bulges of DNA via a combination of conformational selection and induced fit, not by binding to a preorganized site. Analysis of circular dichroism spectra indicates that the degree of induced fit observed is primarily a consequence of optimising van der Waals contacts with the walls of the bulge cavity. The effective recognition of the bulge site on duplex DNA appears to depend to a significant extent on the bent groove space being flexible enough to be able to adopt the geometrically optimal conformation compatible with the wedge-shaped drug molecule, rather than involving 'lock and key' recognition. The spectroscopic results indicate a change of DNA conformation, consistent with an allosteric binding model. Spectroscopic studies with various bulged DNAs also reveal that the binding strength directly correlates with the stability of the bulge structures.


Subject(s)
DNA/metabolism , Nucleic Acid Synthesis Inhibitors/metabolism , Zinostatin/metabolism , Allosteric Site , Antibiotics, Antineoplastic/chemistry , Antibiotics, Antineoplastic/metabolism , Binding Sites , Circular Dichroism , DNA/chemistry , Enediynes , Hydrophobic and Hydrophilic Interactions , Models, Molecular , Nucleic Acid Conformation , Nucleic Acid Synthesis Inhibitors/chemistry , Oligodeoxyribonucleotides/chemistry , Oligodeoxyribonucleotides/metabolism , Zinostatin/analogs & derivatives , Zinostatin/chemistry
18.
J Am Chem Soc ; 124(13): 3216-7, 2002 Apr 03.
Article in English | MEDLINE | ID: mdl-11916399

ABSTRACT

The structure of an unusual covalent adduct formed by thiol-activated neocarzinostatin chromophore (NCS-chrom) and a RNA-DNA hybrid having an overhang of four unpaired residues at the 3'-end of the RNA strand has been elucidated by MS and NMR spectroscopic analyses. Unlike previously characterized adducts formed by NCS-chrom on the sugar residue of the DNA target, this adduct has been found to be on one of the uracil bases in the RNA overhang. Covalent linkage is between C-6 of the post-activated NCS-chrom and C-5 of the uracil. A novel mechanism involving adduction of the NCS-chrom C-6 radical, generated by 2-mercaptoethanol activation, to C-5 of the uracil at the U9 position of the RNA 11-mer, oxidation by dioxygen, reduction by the thiol, and subsequent dehydration is proposed for adduct formation.


Subject(s)
Pyrimidines/chemistry , RNA/chemistry , Antibiotics, Antineoplastic/chemistry , Antibiotics, Antineoplastic/pharmacology , Mercaptoethanol/chemistry , Nuclear Magnetic Resonance, Biomolecular , Uridine Monophosphate/chemistry
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