ABSTRACT
AIMS: To explore the combined effect of yeast proteins and surfactants on bacterial metabolism. METHODS AND RESULTS: Protein-rich cell-free supernatant from heat-shocked yeast Saccharomyces cerevisiae was combined with certain synthetic surfactants. These blends affected the metabolism of a Polyseed inoculum of aerobic bacteria, accelerating CO(2) production and consumption of nutrients from a sterile nutrient broth solution, without a concomitant accumulation of biomass. It is suggested that in the presence of the yeast protein-surfactant complexes, bacterial electron transport is uncoupled from biomass accumulation. The 'uncoupling hypothesis' is supported by experiments with model membranes, in which the same complexes induced proton leak similar to standard chemical uncouplers, such as dinitrophenol, indicating that uncoupling may occur at the stage of generation of the transmembrane pH gradient as the driving force for ATP production. CONCLUSIONS: Yeast protein-surfactant complexes behave as uncouplers of oxidative metabolism in bacteria and appear to do so by increasing proton permeability of membranes. SIGNIFICANCE AND IMPACT OF THE STUDY: Yeast proteins may be of interest as nontoxic, environmentally benign and economically sound agents accelerating oxidative bacterial metabolism while uncoupling it from biomass accumulation. There are actual and potential implications in waste water/soil decontamination, degreasing and other environmental technologies.
Subject(s)
Bacteria/metabolism , Electron Transport/physiology , Heat-Shock Proteins/metabolism , Saccharomyces cerevisiae Proteins/physiology , Surface-Active Agents/pharmacology , Uncoupling Agents/pharmacology , Biomass , Bioreactors , Carbon Dioxide/analysis , Cell Membrane Permeability/drug effects , Hydrogen-Ion Concentration , ProtonsABSTRACT
Recent attention has been given to a reexamination of results from the early Viking missions to Mars that suggested the presence of one or more strong oxidants in Martian soil. Since Fe is one of the main constituents of the Martian surface and Fe(VI) is known to be a highly reactive, strong oxidant, we have made XANES and EXAFS measurements of Fe(II), Fe(III), Fe(IV), and Fe(VI) in solid and solution forms. Results from these studies indicate a preedge XANES feature from Fe(VI) samples similar to that commonly seen from Cr(VI) samples. Results of first shell analysis indicate a linear relationship between the Fe-O bondlength and Fe valence state.
