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1.
Life Sci ; 51(25): PL249-54, 1992.
Article in English | MEDLINE | ID: mdl-1453873

ABSTRACT

The effect of lowering extracellular ion concentration on ultraviolet (UV) light-induced photorelaxation of norepinephrine(NE)-constricted rabbit isolated thoracic aorta was investigated. The magnitude of the photorelaxation response (similar to acetylcholine-induced, but not nitroprusside-induced, relaxation) progressively declined, in the absence of an effect on NE-induced vasoconstriction, as the total extracellular ion concentration was progressively reduced. This diminution in the photorelaxation response was duplicated by isosmotic lowering of the extracellular concentration of Na+, but not other ions, from 145 to 25 mM and was not restored by the replenishment of the Na+ deficiency by equimolar amounts of mannitol or Li+. In contrast, choline fully substituted for Na+. These findings suggest a fundamental difference in the ion dependency (and, hence, the mechanisms) of UV-induced photorelaxation and the vasorelaxations induced by acetylcholine or sodium nitroprusside.


Subject(s)
Muscle Relaxation/radiation effects , Muscle, Smooth, Vascular/radiation effects , Sodium/metabolism , Acetylcholine/pharmacology , Animals , Aorta, Thoracic/metabolism , Aorta, Thoracic/radiation effects , Calcium/metabolism , Chlorides/metabolism , Female , Lithium/metabolism , Muscle, Smooth, Vascular/metabolism , Muscle, Smooth, Vascular/physiology , Norepinephrine/pharmacology , Rabbits , Ultraviolet Rays , Vasoconstriction/drug effects
2.
J Pharmacol Exp Ther ; 257(3): 1136-45, 1991 Jun.
Article in English | MEDLINE | ID: mdl-1646324

ABSTRACT

Captopril, an angiotensin-converting enzyme inhibitor, reportedly can scavenge superoxide anion (O2-), a property attributed to its sulfhydryl group. The present investigation, using rabbit aortic rings precontracted with either norepinephrine or clonidine, was designed to determine whether captopril possesses an endothelium-dependent component of vasodilation related to its ability to protect endothelium-derived relaxing factor (EDRF) from superoxide-mediated destruction. Also studied were enalaprilat, a nonsulfhydryl angiotensin-converting enzyme-inhibitor, superoxide dismutase, and the sulfhydryl compounds glutathione (GSH), N-2-mercaptopropionylglycine (MPG) and N-acetylcysteine (NAC). Captopril, but not enalaprilat, caused dose-dependent relaxations in preconstricted aortic rings containing an intact endothelium. Rings denuded of endothelium were unresponsive to any dose of captopril. Captopril's vasodilation was not related to prostaglandin influence but was associated with an increase in cyclic GMP. Superoxide dismutase, GSH, MPG and NAC also produced endothelium-dependent relaxations similar to captopril. It was also demonstrated that endothelium-dependent relaxations to acetylcholine were enhanced by captopril, GSH, MPG and NAC but not by enalaprilat. In another set of experiments, the ability of captopril to inhibit superoxide-mediated inactivation of EDRF was examined. Pyrogallol, a potent generator of O2-, and superoxide dismutase, a scavenger of O2-, were used as a basis for comparing a possible scavenging effect of captopril. In preconstricted rings, pyrogallol elicited endothelium-dependent contractions that were attenuated by both captopril and superoxide dismutase. Similar effects were found with GSH, MPG and NAC but not with enalaprilat. These results suggest that captopril's endothelium-dependent vasodilation is due to its sulfhydryl group and the ability of the latter to scavenge O2-, thereby protecting EDRF.


Subject(s)
Captopril/pharmacology , Endothelium, Vascular/physiology , Nitric Oxide/physiology , Sulfhydryl Compounds/pharmacology , Vasodilation/physiology , Acetylcholine/pharmacology , Acetylcysteine/pharmacology , Animals , Cyclic GMP/metabolism , Enalaprilat/pharmacology , Glutathione/pharmacology , In Vitro Techniques , Norepinephrine/pharmacology , Pyrogallol/pharmacology , Rabbits , Structure-Activity Relationship , Superoxide Dismutase/pharmacology , Tiopronin/pharmacology , Vasodilation/drug effects
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