ABSTRACT
Staphylopeptides with molecular weight less than 4000 Da, possessing their own specific allergic activity in skin allergic tests. immunoleukolysis test and phagocytosis breaking test were obtained by the acid extraction method.
Subject(s)
Peptides/pharmacology , Staphylococcus aureus/immunology , Allergens/immunology , Allergens/isolation & purification , Allergens/pharmacology , Animals , Guinea Pigs , Hydrogen-Ion Concentration , Molecular Weight , Neutrophils/drug effects , Neutrophils/immunology , Peptides/immunology , Peptides/isolation & purification , Phagocytosis/drug effects , Phagocytosis/immunology , Skin TestsSubject(s)
Construction Materials/adverse effects , Ships , Cells, Cultured , Dose-Response Relationship, Drug , Humans , Methods , Mitosis/drug effects , Paint , RubberABSTRACT
In gel-filtration of Cl. perfringens type A toxin on Sephadex F-100 and F-50 there was revealed relationship between the leukotoxic activity and a high-molecular component. A method of ion-exchange chromatography on a column with DEAE-Sephadex A-25 from the Cl. perfringens toxin there were obtained 8 fractions 3 of which possessed a marked leukotoxic activity: the capacity to destroy neutrophils in the Svejcar and Vancurik test and to-depress the phagocytic activity of leukocytes. Lecithinase and lethal activity was revealed in one of these fractions only. Leukotoxic fractions differed by the capacity to destroy neutrophils and to decrease their phagocytic activity.
