[Synthesis, cloning, and expression in Escherichia coli cells of human alpha1-antitrypsin cDNA]. / Sintez, klonirovanie i ékspressiia v kletkakh Escherichia coli kDNK alpha1-antitripsina cheloveka.

cDNA coding for the full-length human alpha 1-antitrypsin (AAT) and its leader sequence has been cloned and sequenced. DNA sequences encoding the deletion variants and a full-length copy of AAT were cloned and expressed under trp-promoter control. It has been shown that guanine replacing right upstream and downstream the ATG of deletion variant increases the expression to ten-fold. The synthesis of deletion AAT in E. coli was evaluated immunologically and the level of the synthesis was shown to be 4-5% of total cellular protein.

[In vitro processing of the artificial analog of beta-lipotropin]. / Issledovanie protsessinga in vitro iskusstvennogo analoga beta-lipotropina.

The processing of the recombinant analogue of beta-lipotropin (beta-LHP) having 11 additional N-terminal amino acid residues and separated from the hormone by the processing signal, was investigated using rat adrenal secretory granule lysate as a test system of processing "in vitro". It was found that incubation of the beta-LPH analogue with secretory granule enzymes leads to its limited specific degradation with a release of native beta-endorphin. It is concluded that the additional N-terminal amino acids induced no qualitative changes in beta-LPH processing.

[Primary structure of the avian influenza virus A/FVP/Weybridge hemagglutinin gene]. / Pervichnaia struktura gena gemaggliutinina virusa grippa ptits A/FPV/Weybridge.

The complete primary structure of cDNA for hemagglutinin gene of influenza virus A/FPV/weybridge/27 subtype H7 has been determined. Its comparison with the structures of analogous genes from other strains of the same subtype has shown 75% of base changes resulting in silent mutations. This suggests the weak immunological pressing in course of evolution of this subtype strains. The reason for apathogenicity of this avian strain is supposed to be elimination of a glycosylation site present in the strain A/FPV/Rostock/34. The possibility of using the obtained data for construction of the new generation of vaccines is discussed.

[Primary structure of the gene encoding the PB2 protein in influenza virus A/FPB Weybridge]. / Pervichnaia struktura gena belka PB2 virusa grippa A/FPV Veibridzh.

The complete nucleotide sequence of the cloned full-length DNA copy of the avian influenza virus A/FPV Weybridge PB2 gene has been determined.

[Primary structure of the full-length DNA copy of the neuraminidase gene in the avian influenza virus of the N7 antigenic subtype]. / Pervichnaia struktura polnorazmernoi DNK-kopii gena neiraminidazy antigennogo podtipa N7 virusa grippa ptits.

Complete nucleotide sequence of the cloned full-length DNA copy of the influenza virus A/FPV Weybridge (H7N7) neuraminidase gene has been determined.

[Synthesis, cloning and primary structure of cDNA for proopiomelanocortin from the pituitary gland of the mink (Mustella vison)]. / Sintez, klonirovanie i opredelenie pervichnoi struktury kDNK proopiomelanokortina iz gipofiza norki (Mustella vison).

Total RNA was extracted from Mustella vison pituitary gland, and cDNA for proopiomelanocortin mRNA was synthesised and cloned. A 600 b. p. insert encoding for total ACTH, beta LPH and 3'-nontranslated end of the mRNA was sequenced using the Maxam-Gilbert technique.

[Comparative analysis of primary structure of M-genes in remantadine-resistant and remantadine-sensitive strains of influenza virus A/FPV/Weybridge (H7N7) strains]. / Sravnitel'nyi analiz pervichnykh struktur M-genov remantadinustoichivogo i remantadinchuvstvitel'nogo shtammov virusa grippa A/FPV/Weybridge (H7N7).

Full-length DNA copies of M-gene of remantadine-sensitive and remantadine-resistant variants of the influenza virus strain A/FPV/Weybridge (H7N7) have been synthesised and cloned. Complete nucleotide sequences of both cDNAs were determined by the Maxam-Gilbert method. There are three nucleotide substitutions, two of which lead to amino acid changes in M1 and M2 proteins. The existence of M3 protein, a polypeptide 68 amino acids long, encoded by the negative strand of RNA, is suggested. Amino acid changes in M2 and M3 proteins and their relation to the remantadine resistance are discussed.

[Synthesis, cloning and determination of the primary structure of DNA complementary to mRNA of prolactin from the human pituitary gland]. / Sintez, klonirovanie i opredelenie pervichnoi struktury DNK, komplementarnoi mRNK prolaktina iz gipofiza cheloveka.

The poly(A)-containing mRNA from human pituitary and prolactinoma have been purified and translated in the cell-free system from rabbit reticulocytes. mRNA from prolactinoma was shown to be enriched with specific prolactin mRNA. DNA complementary to the prolactin mRNA from human pituitary was obtained and cloned. Sequencing of the 900 bp insert by the Maxam-Gilbert technique suggested the cDNA cloned to cole for the previously published amino acid sequence, mismatches with mRNA from prolactinoma occurring at the third positions of codons and thus not causing amino acid substitutions.

[Expression in Escherichia coli cells of the nucleotide sequence of DNA coding for the bovine lipotropic hormone]. / Ekspressiia v kletkakh Escherichia coli nukleotidnoi posledovatel'nosti DNK, kodiruiushchei lipotropnyi gormon byka.

A cDNA fragment of bovine proopiomelanocortin coding for beta-lipotropic hormone was joined with a promoter and ribosome binding site of B. amyloliquefaciens and cloned in E. coli in pBR 327 plasmid. The level of beta-lipotropin synthesis in bacterial cells transformed by the obtained plasmid was estimated immunochemically. The level of beta-lipotropin production was shown to be 5 mg per liter of bacterial culture.

[Primary structure of the full-size DNA copy of the influenza virus A/Kiev/59/79 (H1N1) PB1 gene protein]. / Pervichnaia struktura polnorazmernoi DNK-kopii gena belka PB1 virusa grippa A/Kiev/59/79 (H1N1).

Nucleotide sequence of the A/Kiev/59/79 influenza virus PB1 gene is reported, thus completing the full-genome primary structure of the recombinant between the virus and laboratory strain A/PR/8/34. The parental strain A/Kiev/59/79 (H1N1) is, in turn, shown to be a natural reassortant inheriting its genes of polymerase complex (PB1, PB2, NP and, in all probability, PA) from contemporary H3N2 influenza virus strains.

[Primary structure of the full-length DNA copy of the influenza virus A/Kiev/59/79 (H1N1) PB2 gene]. / Pervichnaia struktura polnorazmernoi DNK-kopii gena belka PB2 virusa grippa A/Kiev/59/79 (H1N1).

The complete nucleotide sequence of a cloned full-length DNA copy of the A/Kiev/59/79 (H1N1) influenza virus PB2 gene has been determined. This strain is shown to be the natural reassortant which inherited its NP and PB2 genes from the contemporary H3N2 influenza strains.

[Cloning of DNA complementary to mRNA for proopiomelanocortin from the bovine, rat and human hypophysis. Hormonal regulation of proopiomelanocortin mRNA in the rat hypophysis]. / Klonirovanie DNK, komplementarnoi mRNK proopiomelanokortina gipofiza byka, krysy i cheloveka. Gormonal'naia reguliatsiia soderzhaniia mRNK proopiomelanokortina v gipofize krysy.

Cloning of DNA and complementary mRNA of bovine, rat and human proopiomelanocortin (POMC) was carried out. A structural analysis of the cloned cDNA of POMC was performed. Using restriction fragments of bovine, rat and human POMC cloned cDNA, probes for molecular hybridization based on one-chain bacteriophage M13 were made. Using the dot-hybridization technique with labeled [32P] POMC cDNA, the effect of 17 beta-estradiol and adrenalectomy on the POMC mRNA level in rat hypophysis was studied. The hormone was shown to significantly decrease the POMC mRNA content at a concentration of 4 and 8 micrograms per 100 g of body weight 4 hours after injection. Adrenalectomy caused a 2-3-fold increase in the POMC mRNA level in rat hypophysis after a period of 5-7 days. The possibility of polyhormonal control of POMC genome transcription is discussed.

[Synthesis, cloning and primary structure of DNA complementary to mRNA for human pituitary pro-opiomelanocortin]. / Sintez, klonirovanie i opredelenie pervichnoi struktury DNK, komplementarnoi mRNK proopiomelanokortina iz gipofiza cheloveka.

cDNA coding for the human pro-opiomelanocortin (POMC) has been cloned and sequenced. It codes for full size amino acid sequence of POMC and furthermore, contains most part of the 3'-terminal noncoding mRNA region and 60 nucleotides coding for signal peptide.

[Primary structure of the full-size DNA copy of the NP gene of influenza virus A/Kiev/59/79 (H1N1)]. / Pervichnaia struktura polnorazmernoi DNK-kopii gena belka NP virusa grippa A/Kiev/59/79 (H1N1).

The complete nucleotide sequence of the cloned full-length DNA copy of A/Kiev/59/79 (H1N1) influenza virus nucleoprotein gene has been determined. This strain is shown to be the natural recombinant that inherited its nucleoprotein gene from contemporary H3N2-influenza strains. The comparison with other NP-genes reveals the probable localization of antigenic determinants and phosphorylation site of the NP-protein.

[Primary structure of the full-size DNA copy of the hemagglutinin gene of influenza virus A/Kiev/59/79 (H1N1)]. / Pervichnaia struktura polnorazmernoi DNK-kopii gena gemaggliutinina virusa grippa A/Kiev/59/79 (H1N1).

The complete nucleotide sequence of the cloned full-length DNA copy of A/Kiev/59/79 (H1N1) influenza virus hemagglutinin gene has been determined. The comparison with the other hemagglutinin structures reveals the divarication of evolutionary pathway of the H1N1-influenza viruses.

[Primary structure of a full-size DNA copy of the influenza virus A/Kiev/59/79 (H1N1) neuraminidase gene]. / Pervichnaia struktura polnorazmernoi DNK-kopii gena neiraminidazy virusa grippa A/Kiev/59/79 (H1N1).

The complete nucleotide sequence of the cloned full-length DNA copy of the influenza virus A/Kiev/59/79 (H1N1) neuraminidase gene has been determined. The comparison with the other neuraminidases reveals the differences in localization of antigenic determinants between N1 and N2 subtypes and divarication of evolutionary pathways of the modern H1N1-influenza viruses.

[Synthesis, cloning and determination of the primary structure of a full-size DNA copy of the neuraminidase gene from influenza virus type A subtype H1N1]. / Sintez, klonirovanie i opredelenie pervichnoi struktury polnorazmernoi DNK-kopii gena neiraminidazy virusa grippa A podtipa H1N1.

Complete nucleotide sequence of the cloned full-length DNA copy of the influenza virus A (H1N1) neuraminidase gene has been determined. The predicted amino acid sequence is compared with sequences of neuraminidases from other influenza virus strains. A section of the neuraminidase is found to be homologous to the chicken lysozyme catalytic centre.