[The effect of the lectin from Sambucus nigra inflorescences on spontaneous and alkylating agent-induced mutagenesis in mammalian somatic cells]. / Vliianie lektina sotsvetii Sambucus nigra na spontannyi i indutisrovannyi alkiliruiushchim agentom mutagenez v somaticheskikh kletkakh mlekopitaiushchikh.

Data on separation of purified galactose-specific lectin as well as its two polypeptide chains from inflorescences of European elder Sambucus nigra are represented. Their molecule quaternary structure after the pattern a2 b2 is suggested. It was shown that purified protein in concentration 2.0 mg/ml had mutagenic activity and might enhance mutagenic effect of alkylating substance MNNG. Thus, preparations of lectin from inflorescences of European elder influenced on spontaneous and induced by the alkylating agent mutagenicity in mammalian somatic cells in vitro.

[Isolation and characterization of hemagglutinin from the stock culture of Actinomyces streptomycini str. 32-12]. / Vydelenie i nekotorye svoistva gemaggliutinina iz matochnika Actinomyces streptomycini 32-12.

From the culture fluid of Actinomyces streptomycini streptomycin was extracted and then hemagglutinin, a biologically active substance, was isolated. The isolation included ethanol fractionation, sulfate ammonium protein precipitation, elimination of contaminating components via centrifugation, dialysis and subsequent lyophilization. The resultant hemagglutinin showed activity towards animal and human erythrocytes, and blast-transforming and mitogenic activity towards human lymphocytes.