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1.
Biochem Soc Trans ; 41(5): 1201-5, 2013 Oct.
Article in English | MEDLINE | ID: mdl-24059509

ABSTRACT

Proteins that switch between distinct conformational states are ideal to monitor and control molecular processes within the complexity of biological systems. Inspired by the modular architecture of natural signalling proteins, our group explores generic design strategies for the construction of FRET-based sensor proteins and other protein switches. In the present article, I show that designing FRET sensors based on mutually exclusive domain interactions provides a robust method to engineer sensors with predictable properties and an inherently large change in emission ratio. The modularity of this approach should make it easily transferable to other applications of protein switches in fields ranging from synthetic biology, optogenetics and molecular diagnostics.


Subject(s)
Biosensing Techniques , Protein Engineering , Protein Interaction Domains and Motifs/genetics , Proteins/genetics , Fluorescence Resonance Energy Transfer , Proteins/chemistry , Synthetic Biology
2.
Chembiochem ; 14(13): 1553-63, 2013 Sep 02.
Article in English | MEDLINE | ID: mdl-23956201

ABSTRACT

Proper protein folding is a prerequisite for protein stability and enzymatic activity. Although directed evolution can be a powerful tool to investigate enzymatic function and to isolate novel activities, well-designed libraries of folded proteins are essential. In vitro selection methods are particularly capable of searching for enzymatic activities in libraries of trillions of protein variants, yet high-quality libraries of well-folded enzymes with such high diversity are lacking. We describe the construction and detailed characterization of a folding-enriched protein library based on the ubiquitous (ß/α)8 barrel fold, which is found in five of the six enzyme classes. We introduced seven randomized loops on the catalytic face of the monomeric, thermostable (ß/α)8 barrel of glycerophosphodiester phosphodiesterase (GDPD) from Thermotoga maritima. We employed in vitro folding selection based on protease digestion to enrich intermediate libraries containing three to four randomized loops for folded variants, and then combined them to assemble the final library (10¹4 DNA sequences). The resulting library was analyzed by using the in vitro protease assay and an in vivo GFP-folding assay; it contains ∼10¹² soluble monomeric protein variants. We isolated six library members and demonstrated that these proteins are soluble, monomeric and show (ß/α)8-barrel fold-like secondary and tertiary structure. The quality of the folding-enriched library improved up to 50-fold compared to a control library that was assembled without the folding selection. To the best of our knowledge, this work is the first example of combining the ultra-high throughput mRNA display method with selection for folding. The resulting (ß/α)8 barrel libraries provide a valuable starting point to study the unique catalytic capabilities of the (ß/α)8 fold, and to isolate novel enzymes.


Subject(s)
Peptide Library , Protein Folding , Protein Structure, Secondary , Cloning, Molecular , Enzyme Activation , Models, Molecular , Phosphoric Diester Hydrolases/chemistry , Phosphoric Diester Hydrolases/genetics , Phosphoric Diester Hydrolases/isolation & purification , Phosphoric Diester Hydrolases/metabolism , Thermotoga maritima/enzymology
3.
Methods Mol Biol ; 978: 73-92, 2013.
Article in English | MEDLINE | ID: mdl-23423890

ABSTRACT

In the past decade, in vitro evolution techniques have been used to improve the performance or alter the activity of a number of different enzymes and have generated enzymes de novo. In this review, we provide an overview of the available in vitro methods, their application, and some general considerations for enzyme engineering in vitro. We discuss the advantages of in vitro over in vivo approaches and focus on ribosome display, mRNA display, DNA display technologies, and in vitro compartmentalization (IVC) methods. This review aims to help researchers determine which approach is best suited for their own experimental needs and to highlight that in vitro methods offer a promising route for enzyme engineering.


Subject(s)
Enzymes/chemistry , Enzymes/metabolism , Protein Engineering/methods , Directed Molecular Evolution , Enzymes/genetics , Gene Library
4.
Hepatology ; 57(2): 740-52, 2013 Feb.
Article in English | MEDLINE | ID: mdl-22899095

ABSTRACT

UNLABELLED: Bile acids are pivotal for the absorption of dietary lipids and vitamins and function as important signaling molecules in metabolism. Here, we describe a genetically encoded fluorescent bile acid sensor (BAS) that allows for spatiotemporal monitoring of bile acid transport in single living cells. Changes in concentration of multiple physiological and pathophysiological bile acid species were detected as robust changes in Förster resonance energy transfer (FRET) in a range of cell types. Specific subcellular targeting of the sensor demonstrated rapid influx of bile acids into the cytoplasm and nucleus, but no FRET changes were observed in the peroxisomes. Furthermore, expression of the liver fatty acid binding protein reduced the availability of bile acids in the nucleus. The sensor allows for single cell visualization of uptake and accumulation of conjugated bile acids, mediated by the Na(+)-taurocholate cotransporting protein (NTCP). In addition, cyprinol sulphate uptake, mediated by the putative zebrafish homologue of the apical sodium bile acid transporter, was visualized using a sensor based on the zebrafish farnesoid X receptor. The reversible nature of the sensor also enabled measurements of bile acid efflux in living cells, and expression of the organic solute transporter αß (OSTαß) resulted in influx and efflux of conjugated chenodeoxycholic acid. Finally, combined visualization of bile acid uptake and fluorescent labeling of several NTCP variants indicated that the sensor can also be used to study the functional effect of patient mutations in genes affecting bile acid homeostasis. CONCLUSION: A genetically encoded fluorescent BAS was developed that allows intracellular imaging of bile acid homeostasis in single living cells in real time.


Subject(s)
Bile Acids and Salts/metabolism , Fluorescence Resonance Energy Transfer/methods , Organic Anion Transporters, Sodium-Dependent/metabolism , Symporters/metabolism , Animals , Biosensing Techniques/methods , Carrier Proteins , Cell Nucleus/metabolism , Cytoplasm/metabolism , Fluorescent Dyes , Humans , Membrane Glycoproteins , Membrane Transport Proteins/biosynthesis , Organic Anion Transporters, Sodium-Dependent/genetics , Receptors, Cytoplasmic and Nuclear/metabolism , Symporters/genetics , Zebrafish
7.
Trends Biotechnol ; 28(7): 340-5, 2010 Jul.
Article in English | MEDLINE | ID: mdl-20483496

ABSTRACT

Enzymes offer cheap, environmentally responsible and highly efficient alternatives to chemical catalysts. The past two decades have seen a significant rise in the use of enzymes in industrial settings. Although many natural enzymes have been modified through protein engineering to better suit practical applications, these approaches are often insufficient. A key goal of enzyme engineers is to build enzymes de novo - or, 'from scratch'. To date, several technologies have been developed to achieve this goal: namely, computational design, catalytic antibodies and mRNA display. These methods rely on different principles, trading off rational protein design against an entirely combinatorial approach of directed evolution of vast protein libraries. The aim of this article is to review and compare these methods and their potential for generating truly de novo biocatalysts.


Subject(s)
Computational Biology , Enzymes , Industry , Protein Engineering , Animals , Mice , RNA, Messenger/chemistry , RNA, Messenger/metabolism
8.
Biochemistry ; 48(43): 10308-20, 2009 Nov 03.
Article in English | MEDLINE | ID: mdl-19795834

ABSTRACT

The streptococcal coaggregation regulator (ScaR) of Streptococcus gordonii is a manganese-dependent transcriptional regulator. When intracellular manganese concentrations become elevated, ScaR represses transcription of the scaCBA operon, which encodes a manganese uptake transporter. A member of the DtxR/MntR family of metalloregulators, ScaR shares sequence similarity with other family members, and many metal-binding residues are conserved. Here, we show that ScaR is an active dimer, with two dimers binding the 46 base pair scaC operator. Each ScaR subunit binds two manganese ions, and the protein is activated by a variety of other metal ions, including Cd(2+), Co(2+), and Ni(2+) but not Zn(2+). The crystal structure of apo-ScaR reveals a tertiary and quaternary structure similar to its homologue, the iron-responsive regulator DtxR. While each DtxR subunit binds a metal ion in two sites, labeled primary and ancillary, crystal structures of ScaR determined in the presence of Cd(2+) and Zn(2+) show only a single occupied metal-binding site that is novel to ScaR. The site analogous to the primary site in DtxR is unoccupied, and the ancillary site is absent from ScaR. Instead, metal ions bind to ScaR at a site labeled "secondary", which is composed of Glu80, Cys123, His125, and Asp160 and lies roughly 5 A away from where the ancillary site would be predicted to exist. This difference suggests that ScaR and its closely related homologues are activated by a mechanism distinct from that of either DtxR or MntR.


Subject(s)
Bacterial Proteins/chemistry , Bacterial Proteins/metabolism , Manganese/pharmacology , Streptococcus/metabolism , Amino Acid Sequence , Cadmium/pharmacology , Chromatography, Gel , Cobalt/pharmacology , Crystallography, X-Ray , DNA/metabolism , Gene Expression Regulation, Bacterial/drug effects , Gene Expression Regulation, Bacterial/genetics , Molecular Sequence Data , Nickel/pharmacology , Protein Binding/drug effects , Protein Multimerization , Sequence Homology, Amino Acid , Ultracentrifugation , Zinc/pharmacology
9.
Biochemistry ; 45(51): 15359-72, 2006 Dec 26.
Article in English | MEDLINE | ID: mdl-17176058

ABSTRACT

Manganese transport regulator (MntR) is a member of the diphtheria toxin repressor (DtxR) family of transcription factors that is responsible for manganese homeostasis in Bacillus subtilis. Prior biophysical studies have focused on the metal-mediated DNA binding of MntR [Lieser, S. A., Davis, T. C., Helmann, J. D., and Cohen, S. M. (2003) Biochemistry 42, 12634-12642], as well as metal stabilization of the MntR structure [Golynskiy, M. V., Davis, T. C., Helmann, J. D., and Cohen, S. M. (2005) Biochemistry 44, 3380-3389], but only limited data on the metal-binding affinities for MntR are available. Herein, the metal-binding affinities of MntR were determined by using electron paramagnetic resonance (EPR) spectroscopy, as well as competition experiments with the fluorimetric dyes Fura-2 and Mag-fura-2. MntR was not capable of competing with Fura-2 for the binding of transition metal ions. Therefore, the metal-binding affinities and stoichiometries of Mag-fura-2 for Mn2+, Co2+, Ni2+, Zn2+, and Cd2+ were determined and utilized in MntR/Mag-fura-2 competition experiments. The measured Kd values for MntR metal binding are comparable to those reported for DtxR metal binding [Kd from 10(-)7 to 10(-4) M; D'Aquino, J. A., et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 18408-18413], AntR [a homologue from Bacillus anthracis; Sen, K. I. et al. (2006) Biochemistry 45, 4295-4303], and generally follow the Irving-Williams series. Direct detection of the dinuclear Mn2+ site in MntR with EPR spectroscopy is presented, and the exchange interaction was determined, J = -0.2 cm-1. This value is lower in magnitude than most known dinuclear Mn2+ sites in proteins and synthetic complexes and is consistent with a dinuclear Mn2+ site with a longer Mn...Mn distance (4.4 A) observed in some of the available crystal structures. MntR is found to have a surprisingly low binding affinity (approximately 160 microM) for its cognate metal ion Mn2+. Moreover, the results of DNA binding studies in the presence of limiting metal ion concentrations were found to be consistent with the measured metal-binding constants. The metal-binding affinities of MntR reported here help to elucidate the regulatory mechanism of this metal-dependent transcription factor.


Subject(s)
Bacterial Proteins/chemistry , Bacterial Proteins/metabolism , Manganese/chemistry , Manganese/metabolism , Repressor Proteins/chemistry , Repressor Proteins/metabolism , Bacillus subtilis , Binding Sites , Cadmium/chemistry , Cadmium/metabolism , Cations, Divalent , DNA-Binding Proteins/chemistry , DNA-Binding Proteins/metabolism , Electron Spin Resonance Spectroscopy , Protein Binding
10.
Biochemistry ; 44(9): 3380-9, 2005 Mar 08.
Article in English | MEDLINE | ID: mdl-15736948

ABSTRACT

MntR is a metalloregulatory protein that helps to modulate the level of manganese in Bacillus subtilis. MntR shows a metal-response profile distinct from other members of the DtxR family of metalloregulatory proteins, which are generally considered to be iron(II)-activated. As part of an ongoing effort to elucidate the mechanism and metal-selectivity of MntR, several biophysical studies on wild-type MntR and two active site mutants, MntR E99C and MntR D8M, have been performed. Using circular dichroism (CD) spectroscopy, the thermal stability of these proteins has been examined in the presence of various divalent metal ions. Fluorescence intensity measurements of 8-anilino-1-naphthalenesulfonic acid (ANS) were monitored to examine the folding of these proteins in the presence of different metal ions. These experiments indicate that MntR undergoes a significant conformational change upon metal binding that results in stabilization of the protein structure. These studies also show that the MntR D8M active site mutation causes a detrimental effect on the metal-responsiveness of this protein. Fluorescence anisotropy experiments have been performed to quantify the extent of metal-activated DNA binding by these proteins to two different cognate recognition sequences. Binding of MntR and MntR E99C to the mntA cognate sequence closely parallels that of the mntH operator, confirming that the proteins bind both sequences with comparable affinity depending on the activating metal ion. Fluorescence anisotropy experiments on MntR D8M indicate significantly impaired DNA binding, providing additional evidence that MntR D8M is a dysfunctional regulator.


Subject(s)
Bacterial Proteins/chemistry , Metals, Heavy/chemistry , Repressor Proteins/chemistry , Anilino Naphthalenesulfonates/metabolism , Arginine/genetics , Aspartic Acid/genetics , Bacillus subtilis , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Binding Sites/genetics , Circular Dichroism , DNA-Binding Proteins/chemistry , DNA-Binding Proteins/metabolism , Fluorescent Dyes/metabolism , Glutamic Acid/genetics , Hot Temperature , Manganese/chemistry , Manganese/metabolism , Metals, Heavy/metabolism , Methionine/genetics , Protein Conformation , Protein Denaturation , Repressor Proteins/genetics , Repressor Proteins/metabolism
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