The development and results of oral microbiology at the Semmelweis University of Medicine.

The development of oral microbiology at the Semmelweis University of Medicine started around 1980 in the Institute of Microbiology. The teaching of oral microbiology was introduced to the dental educational program, basic research was initiated, appropriate methods learned and used. An intensive collaboration with the Department of Conservative Dentistry developed, which resulted between 1983 and 1995 in about 23 publications (the majority in international journals) and eight lectures, mainly on international forums.

Effect of an antibacterial varnish and amine-fluoride/stannous fluoride (AmF/SnF2) toothpaste on Streptococcus mutans counts in saliva and dental plaque of children.

The purpose of this study was to evaluate the effect of the simultaneous application of a chlorhexidine and thymol-containing varnish (Cervitec) and an amine fluoride/stannous fluoride containing toothpaste (Meridol) on Streptococcus mutans counts in saliva and dental plaque of school children 12-14 years of age, during a six-week period. The children were separated into group 1 (Cervitec varnish + fluoride-containing toothpaste), group 2 (Cervitec varnish + Meridol toothpaste), and group 3 (Meridol toothpaste alone). Over the six weeks the greatest improvement in salivary Streptococcus mutans count occurred in group 2. Overall, a statistically significant decrease in total microbiological count, and Streptococcus mutans was found in all three groups.

[Study of the effectiveness of amine fluoride and stannous fluoride containing toothpaste and mouthwash]. / Aminfluorid és ónfluorid tartalmú fogkrém és szájöblítö mikrobiológiai hatásosságának vizsgálata.

AmF and SnF2 containing toothpastes (equally 750 ppm F-) and mouthrinses (equally 125 ppm F-) from the GABA International Agency were tested. After a five month treatment period (daily two times tooth cleaning), in the saliva of the examined persons the germ counts of the S. mutans and Lactobacillus showed a decreasing tendency, though the change was not statistically significant. The control group used NaF containing cleaners (toothpaste; 1500 ppm F-, mouthrinse; 250 ppm F-) for also a five month treatment period. Within this group the germ counts of the microorganisms of the examined saliva did not change markedly.

Anticariogenic effect of fluoridated milk and water in rats.

The aim of the study was to get further experimental data on the anticariogenic effect of sodium fluoride (NaF) when administered in milk or water. Thirty six weanling Osborne-Mendel rats were divided into two experimental groups (A, B) of 12 rats each and two control groups (C, D) of six rats each. During the experimental period of four weeks all animals were superinfected with Strep. mutans (NCTC 10449), kept in an automatic feeding machine and given a cariogenic diet (MIT 301). Group A received sodium fluoride (NaF) in water (15 ppm) and group B in ultra high temperature treated milk. Groups C and D respectively received plain milk and distilled water. Group A did not show significantly lower caries reduction compared with the control groups. Group B had significantly the lowest caries scores compared with all other groups. Scores in group C (plain milk) were lower than those in groups D (plain water). The results suggest that the anticariogenic effect of NaF is more pronounced when the vehicle is milk instead of water.