ABSTRACT
Plant lectins are carbohydrate-binding proteins, which can interact with cell surfaces to initiate anti-inflammatory pathways, as well as immunomodulatory functions. Here, we have extracted, purified and part-characterized the bioactivity of Jacalin, Frutalin, DAL and PNA, before evaluating their potential for wound healing in cultured human skin fibroblasts. Only Frutalin stimulated fibroblast migration in vitro, prompting further studies which established its low cytotoxicity and interaction with TLR4 receptors. Frutalin also increased p-ERK expression and stimulated IL-6 secretion. The in vivo potential of Frutalin for wound healing was then assessed in hybrid combination with the polysaccharide galactomannan, purified from Caesalpinia pulcherrima seeds, using both hydrogel and membrane scaffolds formulations. Physical-chemical characterization of the hybrid showed that lectin-galactomannan interactions increased the pseudoplastic behaviour of solutions, reducing viscosity and increasing Frutalin's concentration. Furthermore, infrared spectroscopy revealed -OH band displacement, likely caused by interaction of Frutalin with galactose residues present on galactomannan chains, while average membrane porosity was 100⯵m, sufficient to ensure water vapor permeability. Accelerated angiogenesis and increased fibroblast and keratinocyte proliferation were observed with the optimal hybrid recovering the lesioned area after 11â¯days. Our findings indicate Frutalin as a biomolecule with potential for tissue repair, regeneration and chronic wound healing.
Subject(s)
Biocompatible Materials/chemistry , Biocompatible Materials/pharmacology , Galectins/chemistry , Hydrogels/chemistry , Mannans/chemistry , Membranes, Artificial , Wound Healing/drug effects , Animals , Anti-Infective Agents/chemistry , Anti-Infective Agents/pharmacology , Cell Line , Galactose/analogs & derivatives , Humans , Mice , Models, Molecular , Protein Conformation , Toll-Like Receptor 4/chemistry , Toll-Like Receptor 4/metabolismABSTRACT
The effects of annealing on the properties of unfermented (polvilho doce-PD) and fermented (polvilho azedo-PAD) cassava starches were studied. The samples were submitted to annealing treatment for 24, 72, 96, 144, 192 and 240 hours. The annealing treatment changed the main physicochemical (gelatinization, enthalpy and temperature) and rheological properties (swelling power, peak viscosity and pasting temperature) of the both samples. The increase in the gelatinization temperatures and the narrowing of the gelatinization range was attributed to an reinforcement in the packing of the double helices of the amylopectin. The PAD samples seemed to be more affected by this treatment than PD ones. The PAD samples presented some characteristics of a waxy starch, such as increased crystallinity and increased stability of the pastes, which from a technological point of view is very important to diversify further its use in the food industry.
Subject(s)
Food Industry , Food Technology , Starch and FeculaABSTRACT
O objetivo deste trabalho foi determinar os parâmetros técnicos para conservação da polpa de bacuri (Platonia insignis Mart.) pela aplicação de métodos combinados. Os principais obstáculos utilizados foram pH de 3,12 (próprio da fruta), adição de benzoato de sódio (0,500,750 e 1000 ppm) e metabissulfito de sódio (0,200 e 400 ppm), branqueamento a 100ºC por 0,2 e 3 minutos, além de redução da atividade de água (Aa pela adição de (0 por cento, 17,44 por cento e 28,66 por cento) sacarose para obtenção de Aa 0,98, 097 e0,05 que caracterizam alimentos auto-estáveis. Após sete dias de armazenagem a concentração de 1000 ppm de benzoato de sódio apresentou as melhores respostas na inibição do desenvolvimento microbiano em todas as amostras analisadas. Com o mesmo tempo de armazenagem as amostras branqueadas por 2 e 3 minutos apresentaram significativas reduções na tendência ao escurecimento e na contagem de bactérias mesófilas aeróbicas e de bolores e leveduras quando comparadas com a amostra controle. A concentração de 400 ppm de metabissulfito de sódio apresentou maior estabilização da cor após vinte dias de armazenagem em temperatura ambiente nas três Aa selecioandas
