ABSTRACT
Leptospirosis is a zoonotic disease caused by a bacterium of the genus Leptospira that affects domestic animals, including cattle. Despite of this importance in livestock, little is known about the epidemiology of leptospirosis in cattle in Brazil and other tropical countries. In this context, the objective of this study was to perform the culture of urine and kidney (nâ¯=â¯372 animals) of slaughtered bovines of Minas Gerais state (Brazil), in order to obtain pure cultures of Leptospira strains and carry out its characterization by serological (microscopic agglutination test) and molecular techniques (rrs, secY sequencing and variable-number tandem-repeat genotyping). One strain, named UFU 02, was obtained from a male bovine without apparent alterations in ante-mortem examination. The strain was characterized belonging to species Leptospira kirschneri and serogroup Grippotyphosa. The serogroup Grippotyphosa is most often described in wild species, suggesting a link between domestic and wild cycles/environments. Considering the importance of the country in livestock breeding, the knowledge about leptospiral strains could help to control the disease in the herds.
Subject(s)
Cattle Diseases/epidemiology , Cattle Diseases/microbiology , Leptospira/classification , Leptospira/genetics , Leptospirosis/veterinary , Animals , Brazil/epidemiology , Cattle , Molecular Typing , Public Health Surveillance , SerogroupABSTRACT
The Brazilian savanna, also known as Cerrado, is one of the world's richest and most ecologically invaluable tropical savanna regions. There are few studies in Brazil about the diseases that affect the wild canids of this biome, which may be harmful to wildlife populations and public health. The aim of this study was to evaluate the occurrence of antibodies against Leptospira spp. in three Cerrado wild canids species using the microscopic agglutination test (MAT). Serum samples were tested from 19 crab-eating foxes (Cerdocyon thous), 14 maned wolves (Chrysocyon brachyurus), and seven hoary foxes (Lycalopex vetulus), all free-ranging animals found in the municipalities of Araguari and Uberlândia, Minas Gerais State, and Cumari, Goiás State, Brazil. Fourteen (35%) of these samples were seropositive. The most frequent serovars detected in the samples were Copenhageni and Hardjo, but reactions to the serovars Autumnalis, Grippotyphosa, Hebdomadis, Wolffi, and Icterohaemorrhagiae also occurred. Notwithstanding other reported results, this study is the first to report the presence of antibodies against Leptospira spp. in L. vetulus. The three species of wild canids examined may act as potential hosts for several serovars of leptospira in Brazil's savanna environment...
O cerrado é a mais diversificada savana tropical do mundo e constitui grande importância ecológica. Ainda assim, são poucos os estudos realizados no Brasil a respeito das doenças que acometem os canídeos desse bioma, o que causa prejuízos à população de animais selvagens e à saúde pública. Essa pesquisa foi realizada com o objetivo de avaliar a ocorrência de anticorpos contra Leptospira spp. em três espécies de canídeos selvagens do cerrado brasileiro utilizando o teste de soroaglutinação microscópica (SAM). Foram testadas amostras de soro sanguíneo de 19 cachorros-do-mato (Cerdocyon thous), 14 lobos guarás (Chrysocyon brachyurus) e sete raposas-do-campo (Lycalopex vetulus) de vida livre das regiões de cerrado de Araguari e Uberlândia, MG, e Cumari, GO. Dessas 40 amostras, 14 (35%) foram soropositivas. Os sorovares detectados com maior frequência foram Copenhageni e Hardjo, porém evidenciou-se também reação aos sorovares Autumnalis, Grippotyphosa, Hbedomadis, Wolffi e Icterohaemorrhagiae. Esse trabalho foi o primeiro a relatar a presença de anticorpos anti-Leptospira spp. em L. vetulus e verificou-se que as três espécies de canídeos utilizadas nessa pesquisa podem atuar como possíveis hospedeiros de diversos sorovares de leptospiras no ambiente de cerrado...
