Kefir Grains Change Fatty Acid Profile of Milk during Fermentation and Storage.

Several studies have reported that lactic acid bacteria may increase the production of free fatty acids by lipolysis of milk fat, though no studies have been found in the literature showing the effect of kefir grains on the composition of fatty acids in milk. In this study the influence of kefir grains from different origins [Rio de Janeiro (AR), Viçosa (AV) e Lavras (AD)], different time of storage, and different fat content on the fatty acid content of cow milk after fermentation was investigated. Fatty acid composition was determined by gas chromatography. Values were considered significantly different when p<0.05. The highest palmitic acid content, which is antimutagenic compost, was seen in AV grain (36.6g/100g fatty acids), which may have contributed to increasing the antimutagenic potential in fermented milk. Higher monounsaturated fatty acid (25.8 g/100g fatty acids) and lower saturated fatty acid (72.7 g/100g fatty acids) contents were observed in AV, when compared to other grains, due to higher Δ9-desaturase activity (0.31) that improves the nutritional quality of lipids. Higher oleic acid (25.0 g/100g fatty acids) and monounsaturated fatty acid (28.2g/100g fatty acids) and lower saturated fatty acid (67.2g/100g fatty acids) contents were found in stored kefir relatively to fermented kefir leading to possible increase of antimutagenic and anticarcinogenic potential and improvement of nutritional quality of lipids in storage milk. Only high-lipidic matrix displayed increase polyunsaturated fatty acids after fermentation. These findings open up new areas of study related to optimizing desaturase activity during fermentation in order to obtaining a fermented product with higher nutritional lipid quality.

Experimental physeal fracture-separations treated with rigid internal fixation.

Salter-Harris Type-III and Type-IV epiphyseal injuries were created in the distal aspect of the femur in growing rabbits, and the healing process was analyzed both in the absence of any treatment and after treatment with anatomical reduction and fixation with compression with use of a cortical screw. A sham operation was performed on the left knee, to create a control group. Untreated Type-III injuries led to an angular deformity of the femur that became more severe with time. In the group that had an untreated Type-IV injury, a step-off developed on the articular surface and increased with time. Early vascular anastomoses between the epiphysis and the metaphysis preceded the formation of osseous bridges in these lesions. The healing process in the animals that were treated with anatomical reduction and rigid internal fixation occurred without the formation of osseous callus, and no marked abnormalities were discernible in the physis.

Traumatic separation of epiphyses. An experimental study in rats.

Four types of physeal fracture-separations, defined by the Salter-Harris classification, were created experimentally in the proximal physis of the right tibia of immature rats. The four types are: Type I, pure epiphyseal separation; Type II, separation of the epiphysis with a metaphyseal fracture; Type III, partial epiphyseal separation with a vertical fracture of the epiphysis; and Type IV, vertical epiphyseal and metaphyseal fractures. A sham operation performed on the left tibia served as the control. The animals were killed at various intervals up to 25 days after the operation. The findings were assessed by roentgenographic, histologic, and vessel injection methods. For Types I and II morphometric analyses were also performed. The influence of various types of lesions on the growth rate and the healing process was documented. For Types I and II lesions there was a transitory growth arrest and an increased thickening of the zone of hypertrophic cells. The alterations regressed after Day 15, and by day 25, a nearly normal plate was seen. For Type III lesions an angular deformity of the tibia occurred that increased with time. For Type IV lesions, a step-off developed on the articular surface that became more severe with time. Early vascular anastomoses between the epiphysis and the metaphysis led to the formation of bone bridges in Type III and Type IV lesions.

[Veno-occlusive disease of the liver: report of 2 cases in the northeast of Brazil]. / Doenca veno-oclusiva do fígado: registro de dois casos no nordeste do Brasil.

Two cases of veno-occlusive disease of the liver (VOD) are reported in eleven and twelve-year-old children. The number of Brazilian cases of this entity amounts to five, with the addition of the present cases. One patient was in a subacute stage (central hepatic fibrosis) and had her diagnosis based on hepatic biopsy and venography; the other one was necropsied in a cirrhotic stage. Aspects concerning differential diagnosis with other regional hepatic disease, as well as the currently known geographic distribution and etiologic possibilities of VOD were reviewed.

Doenca veno-oclusiva do figado:registro de dois casos no nordeste do Brasil. / Veno-occlusive disease of the liver: report of 2 cases in Northeast of Brazil

Sao relatados dois casos tipicos de doenca veno-oclusiva do figado (DVO) em criancas, elevando a cinco o numero de casos no Brasil. Os pacientes foram diagnosticados na fase subaguda (fibrose hepatica central) e na fase cronica (cirrotica), o primeiro atraves de biopsia, complementada pela venografia hepatica e o segundo atraves de necropsia. Sao comentados aspectos do diagnostico diferencial com outras hepatopatias mais frequentes nesta regiao geografica e revistas as possibilidades etiologicas atualmente conhecidas, bem como a distribuicao mundial da DVO do figado