Effect of Resin Cement at Different Thicknesses on the Fatigue Shear Bond Strength to Leucite Ceramic.

OBJECTIVES: This in vitro study was performed to evaluate fatigue survival by shear test in the union of leucite-reinforced feldspathic ceramic using different cement thicknesses. MATERIALS AND METHODS: Leucite-reinforced glass ceramics blocks were sectioned in 2-mm thick slices where resin cylinders were cemented. The samples were distributed in two experimental groups (n = 20) according to the cement thickness (60 and 300 µm). The specimens of each group were submitted to the stepwise fatigue test in the mechanical cycling machine under shear stress state, with a frequency of 2 Hz, a step-size of 0.16 bar, starting with a load of 31 N (1.0 bar) and a lifetime of 20,000 cycles at each load step. RESULTS: The samples were analyzed in a stereomicroscope and scanning electron microscopy to determine the failure type. There is no significant difference between the mean values of shear bond strength according to both groups. Log-rank (p = 0.925) and Wilcoxon (p = 0.520) tests revealed a similar survival probability in both cement layer thicknesses according to the confidence interval (95%). The fracture analysis showed that the mixed failure was the most common failure type in the 300-µm thickness group (80%), while adhesive failure was predominant in the 60-µm thickness group (67%). The different cement thicknesses did not influence the leucite ceramic bonding in fatigue shear testing; however, the thicker cement layer increased the predominance of the ceramic material failure. CONCLUSION: The resin cement thicknesses bonded to leucite ceramic did not influence the long-term interfacial shear bond strength, although thicker cement layer increased the ceramic material cohesive failure. Regardless the cement layer thickness, the shear bond strength lifetime decreases under fatigue.

Influência de um novo dispositivo acessório de fotopolimerização com fibra óptica e pino de fibra de vidro experimental nas propriedades mecânicas, físicoquímicas e adesão dos cimentos resinosos / Influence of a new light curing device with optical fiber and an experimental glass fiber post on the physicochemical, mechanical properties and adhesion of resin cement

O objetivo deste estudo foi avaliar in vitro a influência de um novo dispositivo fotopolimerização e pino de fibra de vidro nas propriedades mecânicas, físicoquímicas e adesão dos cimentos resinosos. Foram utilizados oitenta dentes bovinos, submetidos ao tratamento endodôntico, distribuídos em 5 grupos (n=16): CD (Pino fibra vidro e cimento dual); PF (Pino perfurado e cimento fotoativado); PD (Pino perfurado e cimento dual); POF (Pino perfurado iluminador de fibra óptica e cimento fotoativado); POD (Pino perfurado iluminador de fibra óptica e cimento dual). Os dentes foram preparados para colocação de um protótipo de pino em fibra de vidro que possui um canal interno de diâmetro regular e com conicidade progressiva. A perfuração permite a inserção da fibra óptica ao longo da extensão do pino a fim de possibilitar a ação da luz ao longo de todo o comprimento do conduto radicular. Após a cimentação, os espécimes foram seccionados perpendicularmente, e obtida 1 fatia de 2 mm de espessura do terço apical, médio e cervical. A avaliação mecânica foi realizada através do ensaio de push-out para determinação da resistência adesiva nos terços cervical, médio e apical seguido pela análise da fratura em estereomicroscópio. Os retentores intrarradiculares foram também submetidos ao teste de flexão de 3 pontos para análise do material preenchedor do pino de fibra (n=10). As análises físico - químicas foram realizadas através da determinação do grau de conversão dos cimentos (RAMAN) e análise em espectroscopia de energia dispersiva (EDS) dos monômeros presentes. A adesão foi analisada pela interface de cimentação pelo MEV e reconstrução 3D do novo sistema através do Micro-CT. Os dados obtidos foram analisados estatisticamente pelo ANOVA (um e dois fatores) e comparação múltipla de Tukey, (p<0,05). Os resultados de resistência adesiva evidenciaram que o terço apical obteve o maior valor de resistência adesiva em comparação ao terço médio (p<0,001), o grupo POD do terço apical foi estatisticamente significante em relação ao grupo CD do terço médio (p<0,001). Na analise individual de cada terço, não houve diferença entre os grupos experimentais e o controle (p>0,05). A falha adesiva entre cimento e dentina foi a mais predominante entre todos os grupos e terços. O pino de fibra de vidro (controle) obteve o maior valor de resistência à flexão (p<0,001), seguido do pino de fibra de vidro perfurado preenchido com cimento resinoso (p<0,001). Os maiores valores de GC foram alcançados pelo grupo POD com 82,3% (cervical) 69,9 % (médio) e 76,21% (apical) e o EDS comprovou a presença de componentes químicos adequados. A análise da adesão do novo pino de fibra de vidro comprova uma excelente adaptação no interior do canal radicular nas regiões cervical, médio e apical. Portanto o novo dispositivo com fibra óptica e pino de fibra de vidro experimental aumentaram as propriedades mecânicas, físico-químicas e adesão do cimento resinoso (AU)

The aim of this study was to evaluate in vitro the influence of a new light curing device with optical fiber and experimental glass fiber post on the physicochemical, mechanical, and adhesion properties of resin cements. Eighty bovine teeth were used, submitted to an endodontic treatment, distributed in 5 groups (n=16): CD (Glass fiber post and dual cement); PF (Perforated post and light-cured cement); PD (Perforated post and dual cement); POF (Fiber optic illuminating with a perforated post and lightcured cement); POD (Fiber optic illuminating with a perforated post and dual cement). The teeth were prepared for placement of a glass fiber post prototype, which has an internal canal of regular diameter and progressive taper. The internal perforation extension allows the insertion of the optical fiber along the entire length of the post in order to allow the action of light along the entire length of the root canal.The specimens were sectioned perpendicularly for the tests, and 1 slice approximately 2 mm thick was obtained from the apical, middle and cervical thirds. The mechanical evaluation was carried out through the push-out test to determine the adhesive bond strength, in the cervical, middle and apical thirds, followed by the fracture analysis under a stereomicroscope, the intraradicular post were also submitted to the 3-point bending test for material analys of the fiber post filler (n=10). The physicochemical analyzes were performed by determining the degree of conversion of the cements (RAMAN) of each sample and the analysis of energy dispersive spectroscopy (EDS) of the monomers present. Adhesion was analyzed by the cementation interface and 3D reconstruction of the new system through micro-CT, and finally, SEM analysis of the adhesive interface. The data obtained were analyzed for normality and statistically by ANOVA (one and two ways) and Tukey's multiple comparison (p<0.05). The adhesive bond strength results showed that the apical third had the highest value of adhesive strength compared to the middle third (p<0.001), and the POD group of the apical third was statistically significant in relation to the CD group of the middle third (p<0.001). In the individual analysis of each third, there was no difference between the experimental and control groups (p>0.05). Adhesive failure between cement and dentin was the most prevalent among all groups and thirds. The conventional post (control) had the highest flexural strength value (p<0.001), followed by the perforated fiberglass post filled with resin cement (p<0.001). The POD group achieved the highest GC values with 82.3% (cervical), 69.9% (medium), and 76.21% (apical) and EDS confirmed the presence of adequate chemical components. The analysis of the adhesion of the new fiberglass post proves an excellent adaptation inside the root canal in the cervical, middle and apical regions. Therefore, the new light curing device with optical fiber and experimental glass fiber post improved the resin cement's mechanical, phycochemical, and adhesion. (AU)

Incorporating N-acetylcysteine and tricalcium phosphate into epoxy resin-based sealer improved its biocompatibility and adhesiveness to radicular dentine.

OBJECTIVE: This in vitro study was designed to evaluate the biocompatibility, adhesiveness, and antimicrobial activity of epoxy resin-based sealer associated with N-Acetylcysteine (NAC) or beta-tricalcium phosphate nanoparticles (ß-TCP) as an experimental retro-filling material. METHODS: Cytotoxicity was assessed using 2,3-Bis-(Methoxy-4-Nitro-5-Sulphophenyl)-2H-Tetrazolium-5-Carboxanilide (XTT) and Sulforhodamine B (SRB) assays after exposing human periodontal ligament fibroblasts to extracts of the materials for 1, 3, or 7 days. For the adhesive resistance test, root canals (48 single-root teeth) were instrumented with Reciproc #40 files (VDW GmbH, Germany) and obturated. After 7 days, the apices were sectioned and a retrograde cavity prepared and filled with the experimental materials (Mineral trioxide aggregate, Epoxy sealer, Epoxy sealer+NAC, and Epoxy sealer+ß-TCP). For the push-out test, one 2-mm thick slice was obtained from the apical third of each specimen. Antimicrobial activity was performed using agar diffusion method. Biofilms were grown in microplates and exposed to the extracts of retro-filled materials, followed by analysis of growth inhibition on agar plates. RESULTS: Epoxy sealer in association with ß-TCP or NAC showed better bond strength while Mineral trioxide aggregate allowed for the lowest adhesion. Mineral trioxide aggregate, Epoxy sealer+ß-TCP, and Epoxy sealer+NAC showed low cytotoxicity. Epoxy sealer was the most cytotoxic. In antimicrobial activity assays, all materials had no effect on Candida albicans. Addition of NAC improved the antimicrobial property of Epoxy sealer against Enterococcus faecalis compared to unmodified Epoxy sealer (P<0.05). SIGNIFICANCE: Incorporating ß-TCP or NAC with Epoxy sealer could improve the adhesiveness and biocompatibility for better use in endodontic therapy.

Avaliação da capacidade de limpeza e biocompatibilidade de diferentes soluções irrigadoras utilizadas em Endodontia / Evaluation of the cleaning capacity and biocompatibility of different irrigation solutions used in Endodontics

Os objetivos deste estudo foram: Artigo 1 ­ Analisar a extrusão apical de hipoclorito de sódio (NaOCl), debris e sua citotoxicidade após o preparo dos canais radiculares utilizando NaOCl líquido ou gel. Artigo 2- avaliar a dissolução de matéria orgânica do NaOCl líquido e gel, e a limpeza das paredes dentinárias após a instrumentação; Métodos: Artigo 1 - A avaliação da extrusão apical de NaOCl e debris foi feita pela espectrofotometria do conteúdo extruído após o preparo biomecânico. A citotoxicidade foi avaliada pela resposta de culturas celulares de fibroblastos de ligamento periodontal (PDFL) frente as soluções irrigadoras extruídas, pelo teste XTT para análise da viabilidade celular. Para isso, oitenta dentes foram instrumentados com limas Reciproc #25 e #40 (VDW Munique, Alemanha) e utilizado NaOCl gel e líquido ativados por ultrassom. Os dados foram analisados estatisticamente pelo teste de ANOVA e as diferenças estatísticas pelo teste de Tukey e Dunn (p<0,05). Artigo 2 - A dissolução de matéria orgânica foi realizada usando cubos de carne com tamanho e peso determinado, os quais foram deixados em contato com 1 mL das amostras dos seguintes grupos: NaOCl gel 3% (ChlorCid V); NaOCl gel 3%(VIM); NaOCl líquido 2,5%; NaOCl líquido 5,25%; Solução fisiológica estéril (SF) (controle) por um período de 3 min, os fragmentos foram removidos e pesados novamente para quantificar a matéria orgânica não dissolvida. A comprovação da limpeza das paredes dos canais foi avaliada através do MEV e da estereomicroscopia para isso, oitenta dentes foram instrumentados com limas Reciproc #25 e #40 (VDW Munique, Alemanha) e utilizado NaOCl líquido e gel ativados por ultrassom. Os dados foram analisados estatisticamente pelo teste de ANOVA e as diferenças estatísticas pelo teste de Tukey (p<0,05); Resultados: Artigo 1 ­ A extrusão apical de hipoclorito ocorreu em todos os grupos, sento estatisticamente significante ao controle de SF. O grupo NaOCl 5,25 % foi o que teve a maior quantidade de hipoclorito nas amostras (4,76 µL) e o NaOCl gel 3% VIM a menor extrusão (2,32 µL) sendo estatisticamente significante. Quando comparado os grupos de NaOCl gel 3%, o VIM teve menor quantidade de hipoclorito nas amostras, comparado ao CV, com relevância estatística. A extrusão apical de debris esteve presente em todos os grupos, sendo maior no grupo da SF, porém não houve diferença estatística entre os grupos. Quanto a citotoxicidade, todos os grupos foram citotóxicos estatisticamente quando comparado ao controle (SF); O NaOCl 5,25% foi o mais citotóxico perante todos os grupos. O NaOCl 2,5% foi o menos citotóxico comparado ao NaOCl 5,25% e o NaOCl gel 3%VIM, sendo estatisticamente significante; Artigo 2- O NaOCl Liq 5,25 % foi o que mais dissolveu matéria orgânica comparado aos demais grupos, sendo estatisticamente significante. Todos os grupos contendo NaOCl foram estatisticamente eficientes em dissolução tecidual comparado ao grupo controle (SF). A avaliação da limpeza das paredes radiculares feita por MEV mostrou melhor eficiência no grupo do NaOCl 5,25% e pior resultado no grupo do NaOCl gel 3% VIM, com menor porcentagem de túbulos dentinários abertos. Conclusão: Artigo 1- Houve a extrusão apical de debris e hipoclorito em todos os grupos experimentais após a instrumentação. Sendo o NaOCl 5,25% o grupo com a maior extrusão de hipoclorito e o grupo do NaOCl gel 3% VIM o com menor extrusão apical comparado aos demais grupos. O NaOCl 5,25% foi o mais citotóxico para os fibroblastos, e o NaOCl 2,5% foi o menos citototóxico, sendo recomendado o seu uso no tratamento endodôntico; Artigo 2 ­ O NaOCl 5,25% foi o grupo com maior capacidade de dissolução tecidual e limpeza das paredes dentinarias, seguido pelo grupo NaOCl gel 3% CV.(AU)

The objectives of this study were: Article 1 - Analyze the apical sodium hypochlorite (NaOCl) extrusion, debris and it´s cytotoxicity after the root canals preparation using liquid or gel NaOCl. Article 2 - Evaluate the tissue dissolution of NaOCl liquid and gel, and it´s cleaning efficiency of dentin walls after instrumentation; Methods: Article 1 - The evaluation of the apical extrusion of NaOCl and debris was done by the spectrophotometry of the extruded contents after the biomechanical preparation. Cytotoxicity was evaluated by the cell cultures response of periodontal ligament fibroblasts (PDFL) against extruded irrigation solutions by the XTT test for cell viability analysis. For this, eight teeth were instrumented with Reciproc # 25 and # 40 files (VDW Munich, Germany) and used NaOCl gel and liquid activated by ultrasound. Data were analyzed statistically by the ANOVA test and the statistical differences by the Tukey and Dunn test (p <0.05). Article 2 - The dissolution of organic matter was carried out using meat cubes of determined size and weight, which were left in contact with 1 mL of samples from the following groups: NaOCl gel 3% (ChlorCid V); NaOCL gel 3% (VIM); NaOCl liquid 2.5%; NaOCl liquid 5.25%; Sterile physiological solution (SP) (control) for a period of 3 min, the fragments were removed and weighed again to quantify the undissolved organic matter. The verification of the cleansing of the canal walls was evaluated through the SEM and the stereomicroscopy for this, eight teeths were instrumented with Reciproc # 25 and # 40 files (VDW Munich, Germany) and used NaOCl gel and liquid activated by ultrasound. Data were analyzed statistically by the ANOVA test and statistical differences by the Tukey test (p <0.05); Results: Article 1 ­ Hypochlorite apical extrusion occurred in all groups, and was statistically significant at SP control. The NaOCl 5.25% group had the highest amount of hypochlorite in the samples (4.76 µL) and the NaOCl gel 3% VIM the lowest extrusion (2.32 µL) was statistically significant. When compared to the 3% NaOCl gel groups, the VIM had lower amount of hypochlorite in the samples, compared to the CV, with statistical relevance. Apical extrusion of debris was present in all groups, being higher in the SP group, but there was no statistical difference between the groups. As for cytotoxicity, all groups were statistically cytotoxic when compared to control (SP); NaOCl 5.25% was the most cytotoxic in all groups. 2.5% NaOCl was the least cytotoxic compared to NaOCl 5.25% and NaOCl 3% VIM gel, being statistically significant; Article 2 -The NaOCl Liq 5.25% was the one that dissolved organic matter more compared to the other groups, being statistically significant. All groups containing NaOCl were statistically efficient in tissue dissolution compared to the control group (SP). The SEM evaluation showed a better efficiency in the NaOCl 5.25% group and a worse result in the NaOCl gel group 3% VIM, with a lower percentage of open dentinal tubules. Conclusion: Article 1 - There was the apical extrusion of debris and hypochlorite in all experimental groups after instrumentation. The NaOCl 5.25% group had the highest hypochlorite extrusion and the NaOCl 3% VIM group had the lowest apical extrusion compared to the other groups. NaOCl 5.25% was the most cytotoxic for fibroblasts, and 2.5% NaOCl was the least cytotoxic, being recommended for endodontic treatment; Article 2 - NaOCl 5.25% was the group with the greatest capacity for tissue dissolution and cleaning of the dentin walls, followed by the NaOCl group 2.5% (AU)