ABSTRACT
In the present report we describe the results from a pilot study aimed at detecting enterovirus sequence in cardiac tissues, obtained through endomyocardial biopsies, from patients suffering from cardiac diseases in the Amazon region. Six samples that were collected from three patients were analysed by RT-PCR showing 3 positive and 3 negative results. These preliminary findings suggest the participation of enteroviruses in the etiology of cardiac diseases, mainly myocarditis, and warrant further and broader local studies on this subject.
Subject(s)
Enterovirus Infections/complications , Enterovirus/isolation & purification , Heart Diseases/virology , Heart/virology , Myocardium/pathology , Adult , Aged , Brazil , DNA, Viral/analysis , Enterovirus/genetics , Enterovirus Infections/pathology , Female , Heart Diseases/etiology , Heart Diseases/pathology , Humans , Male , Polymerase Chain ReactionABSTRACT
No presente trabalho, são mostrados resultados de um estudo piloto direcionado à detecção de seqüências de enterovirus em tecido cardíaco obtido a partir de biópsias endomiocárdicas de pacientes com doenças cardíacas na região Amazônica. Seis amostras coletadas de três pacientes foram analisadas por RT-PCR obtendo-se três espécimes positivos e três negativos. Esses achados preliminares sugerem a participação dos enterovirus na etiologia de doenças cardíacas, principalmente miocardites, e justificam estudos mais amplos nesse assunto.
In the present report we describe the results from a pilot study aimed at detecting enterovirus sequence in cardiac tissues, obtained through endomyocardial biopsies, from patients suffering from cardiac diseases in the Amazon region. Six samples that were collected from three patients were analysed by RT-PCR showing 3 positive and 3 negative results. These preliminary findings suggest the participation of enteroviruses in the etiology of cardiac diseases, mainly myocarditis, and warrant further and broader local studies on this subject.
Subject(s)
Adult , Aged , Female , Humans , Male , Heart Diseases/virology , Heart/virology , Enterovirus/isolation & purification , Enterovirus Infections/complications , Myocardium/pathology , Brazil , Heart Diseases/etiology , Heart Diseases/pathology , DNA, Viral/analysis , Enterovirus/genetics , Enterovirus Infections/pathology , Polymerase Chain ReactionABSTRACT
An epidemic of acute haemorrhagic conjunctivitis in Belém, Pará, Brazil, was investigated. From 83 patients, 73 samples of virus was isolated in cultures of HEP-2 cells from conjunctival swab and throat swab. The virus isolates were identified in Centers for Disease Control, Atlanta, Georgia, USA, as an antigenic variant of coxsackievirus A24. Neutralization Test was done on 56 paired serum samples, using entero virus type 70 (EV70) and virus isolates. Serological conversions for virus isolates were found in 57% (32 patients).
