ABSTRACT
BACKGROUND: Group B streptococcus (GBS) is the main bacteria that infects pregnant women and can cause abortion and chorioamnionitis. The impact of GBS effects on human trophoblast cells remains largely elusive, and actions toward anti-inflammatory strategies in pregnancy are needed. A potent anti-inflammatory molecule, uvaol is a triterpene from olive oil and its functions in trophoblasts are unknown. We aimed to analyze biomechanical and functional effects of inactivated GBS in trophoblast cells, with the addition of uvaol to test potential benefits. METHODS: HTR-8/SVneo cells were treated with uvaol and incubated with inactivated GBS. Cell viability and death were analyzed. Cellular elasticity and topography were accessed by atomic force microscopy. Nitrite production was evaluated by Griess reaction. Nuclear translocation of NFkB p65 was detected by immunofluorescence and Th1/Th2 cytokines by bead-based multiplex assay. RESULTS: GBS at 108â¯CFU increased cell death, which was partially prevented by uvaol. Cell stiffness, cytoskeleton organization and morphology were changed by GBS, and uvaol partially restored these alterations. Nuclear translocation of NFkB p65 began 15â¯min after GBS incubation and uvaol inhibited this process. GBS decreased IL-4 secretion and increased IL-1ß, IFN-γ and IL-2, whereas uvaol reverted this. CONCLUSIONS: The increased inflammation and cell death caused by GBS correlated with biomechanical and cytoskeleton changes found in trophoblast cells, while uvaol was effective its protective role. GENERAL SIGNIFICANCE: Uvaol is a natural anti-inflammatory product efficient against GBS-induced inflammation and it has potential to be acquired through diet in order to prevent GBS deleterious effects in pregnancy.
Subject(s)
Streptococcus agalactiae/pathogenicity , Triterpenes/pharmacology , Trophoblasts/drug effects , Trophoblasts/microbiology , Animals , Biological Transport , Biomechanical Phenomena , Cell Death , Cell Line , Cell Nucleus/metabolism , Cell Survival , Chlorocebus aethiops , Cytokines/metabolism , Female , Humans , NF-kappa B/metabolism , Nitrites/metabolism , Pregnancy , Th1 Cells/metabolism , Trophoblasts/metabolism , Vero CellsABSTRACT
Methods for processing of the sesame seed were tested in order to obtain an edible meal. The nutritive value of this meal was evaluated by chemical and biological analyses. According to the results obtained, it was concluded that the best method is to submit the seeds to a maceration process and to a vapor treatment. The sesame flour protein is of reasonable quality, rich in sulfur amino acids. Although is has a low lysine level, it can be utilized for complementing the amino acid pattern of other mixtures.
Subject(s)
Flour , Food Handling/methods , Seeds , Animals , Body Weight , Male , Nutritive Value , Rats , Rats, Inbred StrainsABSTRACT
The results obtained in the first part of this study demonstrated that the sesame flour protein is of reasonable quality and can be utilized, although it has a low lysine content and is rich in sulfur amino acids. Based on the initial data, the sesame flour was supplemented with other meals: "carioca" beans, pigeon pea, and soybean. Nine diets were prepared and the best combination was that of 50% sesame flour protein and 50% pigeon pea, the PER of which was 2.28. Breads were made with these systems, as follows: 100% sesame flour, 100% wheat flour, 50% sesame flour + 50% wheat flour; 30% sesame flour + 70% wheat flour; 30% sesame flour + 30% soybean flour + 40% wheat flour. These were evaluated through sensory analysis by a grading system. Good acceptance was obtained with the bread prepared with 30% sesame flour + 70% wheat flour. Its external and internal appearance, as well as its organoleptic characteristics were close to the bread, with 100% wheat flour. Sesame flour at the 50% proportion gave a bread of medium quality. The protein mixtures of sesame flour and soybean flour were well accepted. Some of the panel members reported that it had a taste somewhat like integral bread.
Subject(s)
Bread , Flour , Food Handling , Seeds , Animals , Fabaceae , Nutritive Value , Plants, Medicinal , Rats , Rats, Inbred Strains , Glycine maxABSTRACT
Os autores apresentam 35 casos de drenagem do Wirsung, quando da realizacao da esfinfincteroplastia. Realizam, concomitantemente, o estudo bioquimico do suco pancreatico e suas relacoes com a alimentacao.Tecem comentarios sobre a possivel etiologia da pancreatite aguda pos-operatoria e propoem a drenagem do Wirsung como fator fundamental da prevencao desta complicacao Apresentam um caso de pancreatite aguda pos-operatoria laboratorial, que evoluiu satisfatoriamente, e um caso de pancreatite cronica que evoluiu com baixos niveis de enzimas pancreaticas. Analisam os dados bioquimicos do suco pancreatico e a resposta enzimatica sob a influencia da dieta zero, da aspiracao nasogastrica e de varias dietas orais. Enfocam mais particularmente os niveis de amilase e lipase do suco pancreatico
