Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
J Plant Physiol ; 170(16): 1416-23, 2013 Nov 01.
Article in English | MEDLINE | ID: mdl-23747060

ABSTRACT

Gibberellins (GAs) are the plant hormones that control many aspects of plant growth and development, including stem elongation. Genes encoding enzymes related to the GA biosynthetic and metabolic pathway have been isolated and characterized in many plant species. Gibberellin 2-oxidase (GA2ox) catalyzes bioactive GAs or their immediate precursors to inactive forms; therefore, playing a direct role in determining the levels of bioactive GAs. In the present study, we produced transgenic plants of the liliaceous monocotyledon Tricyrtis sp. overexpressing the GA2ox gene from the linderniaceous dicotyledon Torenia fournieri (TfGA2ox2). All six transgenic plants exhibited dwarf phenotypes, and they could be classified into two classes according to the degree of dwarfism: three plants were moderately dwarf and three were severely dwarf. All of the transgenic plants had small or no flowers, and smaller, rounder and darker green leaves. Quantitative real-time reverse transcription-polymerase chain reaction (PCR) analysis showed that the TfGA2ox2 expression level generally correlated with the degree of dwarfism. The endogenous levels of bioactive GAs, GA1 and GA4, largely decreased in transgenic plants as shown by liquid chromatography-mass spectrometry (LC-MS) analysis, and the level also correlated with the degree of dwarfism. Exogenous treatment of transgenic plants with gibberellic acid (GA3) resulted in an increased shoot length, indicating that the GA signaling pathway might normally function in transgenic plants. Thus, morphological changes in transgenic plants may result from a decrease in the endogenous levels of bioactive GAs. Finally, a possibility of molecular breeding for plant form alteration in liliaceous ornamental plants by genetically engineering the GA metabolic pathway is discussed.


Subject(s)
Gene Expression Regulation, Plant , Liliaceae/growth & development , Liliaceae/genetics , Mixed Function Oxygenases/genetics , Plant Proteins/genetics , Agrobacterium/genetics , Breeding , Chromatography, Liquid , Flowers/genetics , Flowers/growth & development , Gene Expression Regulation, Developmental , Gibberellins/administration & dosage , Gibberellins/metabolism , Japan , Lamiaceae/genetics , Mass Spectrometry , Microscopy, Electron, Scanning , Mixed Function Oxygenases/metabolism , Plant Leaves/genetics , Plant Leaves/growth & development , Plant Proteins/metabolism , Plants, Genetically Modified/genetics , Plants, Genetically Modified/growth & development , Polymerase Chain Reaction , Transformation, Genetic
SELECTION OF CITATIONS
SEARCH DETAIL
...