ABSTRACT
AuNTs@AuHg alloy with well-regulated SERS properties was proposed, which displayed wonderful SERS intensity and effective salt resistance. Using miRNA-21 as a model analyte and combining with DSN-assisted amplification, a dual signal amplification strategy for ultrasensitive miRNA biosensing with a low detection limit (0.53 fM) and satisfactory selectivity was designed.
Subject(s)
Biosensing Techniques , MicroRNAs , Alloys , Limit of Detection , MicroRNAs/geneticsABSTRACT
Magnesium ammonium phosphate (MAP) precipitation was introduced to remove ammonium nitrogen (NH4(+)-N) in autothermal thermophilic aerobic digestion (ATAD) in this study. The dosing time of MgCl2 · 6H2O and NaH2PO4 · 2H2O will influence the removal efficiency of ammonium nitrogen greatly, and the time interval of 2nd, 7th, 12th day were chosen in ATAD process. The lowest NH4(+)-N concentration was found in the 2nd day dosing digester, and 38.37% of VS removal rate was obtained after 12 days digestion, which achieved stabilization 9 days earlier than the non-dosing digester. It revealed that removal of ammonium nitrogen could accelerate the sludge stabilization process. Meanwhile, 49.30% of VS removal rate was found in the 2nd day dosing digester in the 21st day, much higher than that in the non-dosing digester, the 7th day dosing digester, and the 12th day dosing digester, with the corresponding value of 38.37%, 38.38% and 37.04%, respectively.