ABSTRACT
A taxonomic study was carried out on strain yzlin-01T, isolated from Dongshan Island seawater. The bacterium was Gram-stain-negative, catalase-positive, oxidase-negative, rod-shaped, and motile by polar flagella. Growth was observed at temperatures of 10-40 °C, at salinities of 0.5-18â%, and at pH of 6-10. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain yzlin-01T belonged to the genus Halomonas, with the highest sequence similarity to Halomonas malpeensis YU-PRIM-29T (96.7â%), followed by Halomonas johnsoniae T68687T (96.4â%) and Halomonas gomseomensis M12T (96.4â%), and other species of the genus Halomonas (93.4-96.3â%). The ANI and digital DNA-DNA hybridization estimate values between strain yzlin-01T and the closest type strain Halomonas malpeensis YU-PRIM-29T were 77.44 and 21.6â%, respectively. The principal fatty acids were summed feature 8 (consisting of C18â:â1 ω7c and/or C18â:â1 ω6c; 55.7â%), C16â:â0 (20.6â%), C12â:â0 3-OH (6.8â%), summed feature 3 (consisting of C16â:â1 ω7c and/or C16â:â1 ω6c; 5.1â%). The G+C content of the chromosomal DNA was 60.0 molâ%. The respiratory quinone was identified as Q-9 (100â%). Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, aminophospholipid, and three unidentified phospholipids were present. Combined genotypic and phenotypic data suggest that strain yzlin-01T represents a novel species within the genus Halomonas, for which the name Halomonas dongshanensis sp. nov. is proposed, with the type strain yzlin-01T (=GDMCC 1.3202T=KCTC 92467T).
