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1.
Genet Mol Res ; 14(3): 9423-36, 2015 Aug 14.
Article in English | MEDLINE | ID: mdl-26345876

ABSTRACT

Loquat (Eriobotrya japonica Lindl.), which originates from the cooler hill regions of southwestern China, is a typical subtropical evergreen tree. Loquat is one of the most important economic crops in China, but the available genomic information is very limited. Here, we present the first deep transcriptomic analysis of loquat. De novo assembly generated 116,723 contigs and 64,814 unigenes using Illumina sequencing technology. A total of 45,739 unigenes were annotated by Nr, GO, and COG datasets. In addition, we analyzed the gene expression profiles of loquat fruit under low temperature stress and 4017 differential expressed genes were identified. We found that the unigenes involved in the brassinosteroid biosynthesis and phosphatidylinositol signaling systems were upregulated, indicating that they have an important role in the resistance of plants to low temperature. Our results provide an invaluable resource for identification of specific genes and proteins involved in loquat development and response to low temperatures.


Subject(s)
Cold Temperature , Eriobotrya/genetics , Gene Expression Regulation, Plant , Stress, Physiological , Transcriptome , Computational Biology , Eriobotrya/metabolism , Gene Expression Profiling , High-Throughput Nucleotide Sequencing , Metabolic Networks and Pathways , Molecular Sequence Annotation , Phytosterols/metabolism , Signal Transduction
2.
Int J Oral Maxillofac Surg ; 39(1): 29-35, 2010 Jan.
Article in English | MEDLINE | ID: mdl-19906510

ABSTRACT

Expression of Id-1 (inhibitors of DNA binding/differentiation protein 1) and TSP-1 (thrombospondin-1) in mucoepidermoid carcinoma and their relationship to pathological features and prognosis was studied. Moderately and poorly differentiated groups had significantly higher Id-1 positive expression rate (p<0.05) than well differentiated carcinoma. Stages III-IV showed significant increase of Id-1 positive expression rate (p<0.05) compared with stages I and II. Id-1 positive expression was significantly higher in patients with cervical lymph node metastasis or relapse at 5 years (p<0.05). After that, patients with negative Id-1 expression had significantly higher tumor-free survival than patients with positive expression (p<0.05). Correlation between the expression of Id-1 and TSP-1 in mucoepidermoid carcinoma was negative (p<0.05). Poorly differentiated groups show significantly lower TSP-1 positive expression rate than well differentiated groups (p<0.05). No significant differences of TSP-1 positive expression were detected with clinical stage. TSP-1 positive expression was significantly lower in patients with cervical lymph node metastasis or relapse at 5 years (p<0.05). After 5 years, patients with positive TSP-1 expression had significantly higher tumor-free survival than patients with negative TSP-1. Positive Id-1 expression is associated with high malignancy/poor prognosis; positive TSP-1 expression is associated with low malignancy/good prognosis. Protein expression status may help assess tumor malignancy and patient prognosis.


Subject(s)
Carcinoma, Mucoepidermoid/pathology , Inhibitor of Differentiation Protein 1/analysis , Salivary Gland Neoplasms/pathology , Thrombospondin 1/analysis , Adult , Aged , Biomarkers, Tumor/analysis , Carcinoma/pathology , Carcinoma, Mucoepidermoid/secondary , Cell Differentiation , Coloring Agents , Disease-Free Survival , Female , Follow-Up Studies , Gene Expression Regulation, Neoplastic , Humans , Immunohistochemistry , Lymphatic Metastasis/pathology , Male , Middle Aged , Neoplasm Recurrence, Local/pathology , Neoplasm Staging , Parotid Gland/pathology , Prognosis , Retrospective Studies , Submandibular Gland/pathology , Survival Rate
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