Effective adsorption of bisphenol A from aqueous solution using phosphoric acid-assisted hydrochar.

Sycamore leaf biochar (PSAC) was prepared by a two-step phosphoric acid-assisted hydrothermal carbonization combined with a short-time activation method. The characterization results showed that the introduction of phosphoric acid molecules and thermal activation resulted in a substantial increase in the specific surface area (994.21 m2/g) and microporous capacity (0.307 cm3/g) of PSAC. The batch adsorption results showed that the adsorption process of PSAC on bisphenol A (BPA) was best described by the pseudo-second-order kinetic model and Sips isothermal model, with a maximum adsorption capacity of 247.42 mg/g. The adsorption of BPA onto PSAC was determined to be a spontaneous endothermic process. The equilibrium adsorption capacity of PSAC exhibited an upward trend with increasing initial BPA concentration and temperature while decreasing with higher adsorbent dosage and pH value. Coexisting cations and humic acids in water have little impact on the adsorption performance of PSAC for BPA. The adsorption mechanism of BPA by PSAC was mainly governed by pore filling and hydrogen bonding interactions, π-π interactions, and intraparticle diffusion. Furthermore, PSAC demonstrated good reusability by its sustained adsorption capacity of BPA, which remained at 82.6% of the initial adsorption capacity even after four adsorption-desorption cycles. These findings highlight the potential of utilizing low-cost sycamore leaf biochar as an effective adsorbent for the removal of the endocrine disruptor BPA.

Effective immobilization of bisphenol A utilizing activated biochar incorporated into soil: combined with batch adsorption and fixed-bed column studies.

This study presented the mixture of biochar and soil for removal of bisphenol A (BPA) to assess environmental remediation ability. Using phoenix tree leaves as biomass and phosphoric acid as activator, after one-step hydrothermal and short-term activation, the eventual solid product was phosphoric acid hydrothermal activated carbon (HPC). The characterizations showed that HPC had the high specific surface (994.21 m2·g-1), and large unsaturated esters and hydroxyl groups. The saturated adsorption capacities of batch and column adsorption for the addition of 0.5% HPC to soil were 0.790 mg·g-1 and 67.23 mg·kg-1, while to the natural soil were 0.236 mg·g-1 and 8.75 mg·kg-1, respectively. The adsorption kinetics and thermodynamic analysis indicated that the adsorption process utilizing HPC incorporated into soil was a chemical reaction rate-controlled, physical-dominated multilayer adsorption, and spontaneous endothermic. Also, batch adsorption experiments and analysis were performed under different pH levels, HPC contents, organic acid concentrations, and cationic strengths. Successively, fixed-bed column experiments were carried out with and without the HPC; the results showed that the wide mass transfer zone led to the effective fixation of BPA, and the organic acid had no obvious effect on the fixation of BPA when the 1.0% HPC mixed with soil. Finally, through characterizations and data analysis, the enhanced adsorption of BPA by HPC mixed with soil mainly relied on π-π interaction, hydrogen bonding, followed by electrostatic attraction and pore filling.

Analysis of heavy metals in the conversion of lake sediment and restaurant waste by black soldier fly (Hermetia illucens).

The risk posed by heavy metals makes it difficult to dispose of sediment contaminants from dredging lakes in China. Black soldier fly (Hermetia illucens) can convert organic waste, such as restaurant waste and lake sediment, to high-value-added protein feed and fertilizer. Experimental groups were formed in this study to explore the conversion of heavy metals present in the mixture of restaurant waste and lake sediment by black soldier fly larvae (BSFL). The results demonstrated that BSFL could survive in pure sediment with an 84.76% survival rate. Relative to the substrate, BSFL could accumulate 70-90% zinc (Zn), chromium (Cr), copper (Cu), and 20-40% cadmium (Cd) and lead (Pb). The experimental group 2:3, with 40% lake sediment and 60% restaurant waste, was the best group after conversion for 15 days, which showed a 95.24% survival rate of BFSL, 82.20 mg average weight of BFSL, 8.92 mm average length of BFSL, with varying content of heavy metals such as Cu (43.22 mg/kg), Zn (193.31 mg/kg), Cd (1.58 mg/kg), Cr (25.30 mg/kg) Cr, and Pb (38.59 mg/kg) in BSFL. Furthermore, the conversion residue conforms to the relevant standards of organic fertilizer in China and can be used as organic fertilizer. Overall, the present study shows that black soldier flies can improve the resource utilization of lake sediment, especially by reducing the effect of heavy metals.

A novel carbonized derivative of Fe-Cu bimetallic organic framework (Fe-Cu-MOF@C): preparation and optimization.

A carbon derivative with Fe-Cu bimetallic organic framework (Fe-Cu-MOF@C) was prepared by microwave synthesis and pyrolysis. Using potassium persulfate (PS) as oxidant and 2,4-dichlorophenol (2,4-DCP) as target pollutant, the optimal preparation conditions of Fe-Cu-MOF@C were studied. The factors affecting the synthesis of Fe-Cu-MOF include microwave power, microwave time, microwave temperature, the molar ratio of metal ions to organic ligands, the molar ratio of iron and copper, etc. In addition, the influence of pyrolysis temperature on the performance of Fe-Cu-MOF@C cannot be ignored. The results show that Fe-Cu-MOF@C has the best catalytic performance when the microwave time is 30 min, the microwave power is 600 W, the microwave temperature is 150 °C, the molar ratio of (Fe2+ + Cu2+)/H2BDC is 10:3, the molar ratio of Fe2+/Cu2+ is 10:1, and the pyrolysis temperature is 700 °C. After 90 min of reaction, 2,4-DCP was completely removed. Repeatable experiments show that Fe-Cu-MOF@C has good stability and its service life can be restored by heat treatment. In this study, a heterogeneous catalyst with strong catalytic capacity, high stability and easy recovery was prepared by a simple and efficient process, which is conducive to the development of advanced oxidation technology and the progress of water environmental protection.

Removal mechanism of persistent organic pollutants by Fe-C micro-electrolysis.

The degradation of persistent organic pollutants (POPs) in the simulated wastewaters was investigated by Fe-C micro-electrolysis system. With phenanthrene (PHE) and 2,4-dichlorophenol (2,4-DCP) as target pollutants, different iron-carbon (Fe-C) micro-electrolysis systems have been established. The effects of initial pH, Fe/C mass ratio, and intake air flow on the degradation and mineralization of PHE and 2,4-DCP were studied. At the initial pH of 5.0, Fe/C of 1.5:1, and an aeration flow rate of 1.5 L/min, after 120 min of reaction, the removal efficiency of FHE and COD was 94.3% and 73%, respectively. Under the conditions of initial pH is 3.0, Fe/C is 1:2, aeration flow rate of 1.5 L/min, and reaction time of 90 min, the best removal efficiency of 2,4-DCP can be obtained in the Fe-C micro-electrolysis system as 97% and COD removal efficiency can reach 76%. The results of kinetic studies show that the Fe-C micro-electrolysis process of PHE and 2,4-DCP follows pseudo-first-order kinetics. Commercial activated carbon (AC) was used for comparison under the same condition. The results indicated that the removal rate of organic pollutants and chemical oxygen demand (COD) of Fe-C micro-electrolysis were superior to that of AC. Analyze the structure of iron after reaction by SEM and XRD. The degradation pathway and mechanism for PHE and 2,4-DCP were proposed based on LC-MS analyses of treated wastewater.

Laccase immobilized on amino-functionalized magnetic Fe3O4-SiO2 core-shell material for 2,4-dichlorophenol removal.

In this study, an amino-functionalized magnetic silica microsphere material (Fe3O4-SiO2-NH2) was prepared. Using glutaraldehyde as a cross-linking agent, Trametes versicolor laccase was adsorbed-covalently bonded and immobilized on the material to prepare Laccase @ Fe3O4-SiO2. In addition, the materials were characterized and analysed by SEM, TEM, XRD, FT-IR and VSM. Finally, the thermal inactivation dynamics of immobilized laccase in polar/non-polar/toxic systems and the adsorption and degradation of 2,4-DCP were studied. The results showed that Laccase @ Fe3O4-SiO2 under the optimal conditions (pH 6, temperature 65°C, initial concentration of 2,4-DCP 10 mg/L), the removal rate was as high as 81.6%. Moreover, compared with free laccase, immobilized laccase had good tolerance under low pH and high-temperature conditions, and storage stability was also greatly improved. After repeated use for 7 times, Laccase @ Fe3O4-SiO2 can still maintain 59% removal rate of 2,4-DCP, which gives it the potential for industrial applications.

Trends of Diverse POPs in Air and Water Across the Western Atlantic Ocean: Strong Gradients in the Ocean but Not in the Air.

Oceans have remained the least well-researched reservoirs of persistent organic pollutants (POPs) globally, due to their vast scale, difficulty of access, and challenging (trace) analysis. Little data on POPs exists along South America and the effect of different currents and river plumes on aqueous concentrations. Research cruise KN210-04 (R/V Knorr) offered a unique opportunity to determine POP gradients in air, water, and their air-water exchange along South America, covering both hemispheres. Compounds of interest included polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs), polybrominated diphenylethers (PBDEs), and polycyclic aromatic hydrocarbons (PAHs). Remote tropical Atlantic Ocean atmospheric concentrations varied little between both hemispheres; for HCB, BDEs 47 and 99, they were ∼5 pg/m3, PCBs were ∼1 pg/m3, α-HCH was ∼0.2 pg/m3, and phenanthrene and other PAHs were in the low 100s pg/m3. Aqueous concentrations were dominated by PCB 52 (mean 4.1 pg/L), HCB (1.6 pg/L), and ß-HCH (1.9 pg/L), with other compounds <1 pg/L. Target PCBs tended to undergo net volatilization from the surface ocean, while gradients indicated net deposition for a-HCH. In contrast to atmospheric concentrations, which were basically unchanged between hemispheres, we detected strong gradients in aqueous POPs, with mostly nondetects in the tropical western South Atlantic. These results highlight the importance of currents and loss processes on ocean scales for the distribution of POPs.

Degradation of 2,4-dichlorophenol contaminated soil by ultrasound-enhanced laccase.

In this paper, ultrasound was used to enhance the degradation effect of laccase for 2,4-dichlorophenol (2,4-DCP) in soil. The degradation effect and mechanism of the ultrasound-enhanced laccase were investigated. From the results, the degradation rate of 2,4-DCP can reach as high as 51.7% under the following conditions: reaction period was 21 h, pH = 5.5, ultrasound power was 240 W, duty cycle was 50%, and moisture content was 50%. Using the ultrasound-enhanced laccase, the degradation rate of 2,4-DCP was significantly higher than that using only laccase or only ultrasound. In addition, when ultrasound was used, the optimum pH for the degradation of 2,4-DCP using laccase was increased, making the degradation technology more practical. The analysis results from high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) revealed the degradation pathway of 2,4-DCP in soil: first, 2,4-DCP gradually became phenol through dechlorination, then the small molecular organic matter was generated from the hydroxyl radical or laccase reaction.

Adsorption behavior of 2,4-DCP by rice straw biochar modified with CTAB.

In this study, rice straw was used as the raw materials of biomass carbon to prepare biochar at different temperatures (400°C, 500°C, 600°C, 700°C, and 800°C). In addition, cetyl trimethyl ammonium bromide (CTAB) modified biochar was used to treat 2,4-dichlorophenol (2,4-DCP) in water. The influences of adsorbent dosage, solution pH, adsorption time, and initial solubility of the 2,4-DCP solution on the adsorption properties were investigated. The physicochemical properties of biochar were investigated using SEM, FT-IR, BET surface area, and pore size analysis. The results showed that the pyrolysis temperature had a great influence on the biochar structure. CTAB provided hydrophilic and hydrophobic groups to the modified biochar, which had increased adsorption capacity comparing to unmodified biochar. The pH also had a significant effect on the adsorption performance of biochar, and the adsorption performance of biochar decreased significantly under alkaline conditions. The maximum adsorption capacities of modified biochar and unmodified biochar were 59.81 and 20.89 mg/g, respectively, indicating that the adsorption capacity of modified biochar was significantly higher than that of unmodified biochar. The adsorption process of 2,4-DCP by rice straw biochar conformed to the Second-order kinetic model and the Freundlich isotherm adsorption model.

The study on adsorption behavior of 2,4-DCP in solution by biomass carbon modified with CTAB-KOH.

In this study, rice straw was used to prepare biomass carbon, which was modified with KOH and cetyltrimethylammonium bromide (CTAB) to obtain modified biomass carbon (MBC). The biomass carbon (BC) before and after modification was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), thermogravimetric analysis (TGA) and Fourier transform infrared spectroscopy (FT-IR), and the surface morphology, crystal structure and surface group characteristic BC were explored. The specific surface area and micropores of the modified biomass carbon increased significantly, the crystallinity was higher, and the pore structure was more clearly found. The adsorption performance of MBC for 2,4-dichlorophenol (2,4-DCP) was investigated. The results showed that under the best adsorption conditions ((2,4-DCP concentration (200 mg/L), MBC dosage (50 mg), pH (5.5), and loading time (60 min), temperature (room temperature)), the removal rate of 2,4-DCP was up to 42.5%, and adsorption capacity was 85.13 mg/g. The adsorption of 2,4-DCP on MBC materials was better explained by the pseudo-second-order kinetic model and the Langmuir adsorption isotherm model. It was believed that the adsorption of 2,4-DCP by MBC was the monolayer adsorption process on the uniform surface of MBC at high concentration, and there was no interaction between the 2,4-DCP and MBC adsorbate during this process.

[Enhancement of human insulin precursor production by increasing the copy number in Pichia pastoris].

OBJECTIVE: The aim of this study was to further increase the yield of insulin precursor by Pichia pastoris. METHODS: For this, we transformed the expression vector pPICZalpha-IP into P. pastoris X-33 using electroporation and screened two mutant strains B4 and S6 on the YPD plate containing 100 microg/mL zeocin. Both could overexpress human insulin precursor. Taking B4 and S6 as start strains, we repeatedly transformed SacI linearizing pPICZalpha-IP into P. pastoris X-33 by electroporation, then screened a new mutant strain 2B4 (with 7 copies) on the 1000 microg/mL zeocin YPD plate. RESULTS: After cultivation, the human insulin precursor yield of 2B4 strain was 2.7 fold higher than that of B7. Meanwhile, the cell growth was not inhibited. The target gene transcription level of 2B4 was 2 fold higher than that of B7 by real-time quantification PCR. CONCLUSION: The strategy of combining resistance screening and repeated electroporation was efficient to increase the copy number of target gene, so as to facilitate higher transcription level and enhance objective recombinant protein yield.

Organochlorine pesticides (OCPs) in soils and sediments, southeast China: A case study in Xinghua Bay.

This study evaluated the contamination status and distribution of organochlorine pesticides (OCPs) between Xinghua Bay and adjacent watersheds in Putian region, southeast China. Twenty-five surface soil samples and two sediment cores were collected from two watersheds and the Xinghua Bay, respectively. Results showed that the concentrations of OCPs in samples of the Mulan River Watershed (MRW), the Qiulu River Watershed (QRW), the inner bay core (IBC) and the open bay core (OBC) were in the range of 4.96-38.20ng/g, 4.62-22.80ng/g, 1.84-80.46ng/g and 1.87-23.43ng/g, respectively. The mean concentration of OCPs was in an order: IBC>MRW>QRW>OBC. The higher concentration of OCPs in recent periods may suggest that a certain amount of OCPs were still input to this area.

Historical contamination and sources of organochlorine pesticides in sediment cores from Quanzhou Bay, Southeast China.

Three sediment cores were collected from the top to the mouth of Quanzhou Bay, Southeast China, in order to establish sources and historical trends of organochlorine pesticides (OCPs) in the region. The spatial distribution of OCPs in surface sediments implies that Quanzhou Bay received the contamination inputs not only from rivers near the shore, but also from outside the bay. The variation profiles of concentrations clearly showed that OCPs were widely used between 1960s and 1980s in China. A recent increasing trend was found in all cores despite their ban in China in 1983. Different ratios of (DDD+DDE)/DDTs indicated that DDTs at the top of the bay were mainly derived from long-term weather soils, while DDTs near the mouth of the bay were mainly derived from fresh inputs from outside the bay. Higher percentage of gamma-HCH in HCHs deposited after 1990 implies that lindane may have been used recently around Quanzhou Bay.