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1.
Lab Chip ; 23(17): 3874-3881, 2023 08 22.
Article in English | MEDLINE | ID: mdl-37539696

ABSTRACT

Electrophoresis is one of the most powerful techniques to separate nucleic acids or protein molecules. The recovery of purified components from the gel is key to downstream analysis or function study. Here, we provide a cost-effective electroeluter in both homemade and module-assembled versions. The recovery yield can reach as high as >90% for single-stranded DNA (ssDNA), double-stranded DNA (dsDNA), and protein in practical testing, which outperforms a commercial kit as well as a purchased electroeluter. It fully addresses the existing concerns in this field. First of all, for almost all kits, there remains ambiguity in recovering ssDNA to satisfy specific demands, which is generally ignored. Secondly, the recovery of dsDNA from agarose gel with consumables is vulnerable to a lot of factors and involves chemicals/materials that are not friendly to the environment and operating personnel. Thirdly, recovery from polyacrylamide matrices is very difficult, and the most exploited diffusion method through crush-and-soak suffers from low yield even after a long-time diffusion. Lastly, there is a universal problem in scaling up, especially for commercial electroeluters. The present electroelution method addresses the above issues, and it is believed that it will facilitate associated research and find widespread application.


Subject(s)
DNA, Single-Stranded , DNA , Electrophoresis , Cost-Effectiveness Analysis , Diffusion
2.
Int J Mol Sci ; 23(20)2022 Oct 20.
Article in English | MEDLINE | ID: mdl-36293483

ABSTRACT

Natriuretic peptide receptor 1 (NPR1) serves as a modulator of vascular endothelial homeostasis. Interactions between monocytes and endothelial cells may initiate endothelium dysfunction, which is known as an early hallmark of atherosclerosis. In this study, we performed RNA-sequencing analysis for the aorta of Npr1 knockout (Npr1+/-) mice and found that differentially expressed genes were significantly related to cell adhesion. This result was supported by an increased expression of intercellular adhesion molecule 1 (ICAM-1) in the aortic endothelium of Npr1+/- mice. Moreover, we observed that the knockdown of NPR1 increased ICAM-1 expression and promoted THP-1 monocyte adhesion to human umbilical vein endothelial cells (HUVECs). NPR1 overexpression decreased ICAM-1 expression and inhibited the adhesion of monocytes to HUVECs treated by TNF-α (a cell adhesion inducer). Further analysis showed that adhesion-related genes were enriched in the focal adhesion signaling pathway, in which integrin beta 4 (Itgb4) was determined as a key gene. Notably, ITGB4 expression increased in vascular endothelium of Npr1+/- mice and in NPR1-knockdown HUVECs. The deficiency of ITGB4 decreased ICAM-1 expression and attenuated monocyte adhesion to NPR1-knockdown endothelial cells. Additionally, a reduced NPR1 and an increased ITGB4 expression level were found in an atherosclerosis mouse model. In conclusion, our findings demonstrate that NPR1 deficiency increases vascular endothelial cell adhesion by stimulating ITGB4 expression, which may contribute to the development of atherosclerosis.


Subject(s)
Atherosclerosis , Intercellular Adhesion Molecule-1 , Humans , Mice , Animals , Intercellular Adhesion Molecule-1/genetics , Intercellular Adhesion Molecule-1/metabolism , Endothelium, Vascular/metabolism , Tumor Necrosis Factor-alpha/metabolism , Monocytes/metabolism , Cell Adhesion/genetics , Vascular Cell Adhesion Molecule-1/metabolism , Human Umbilical Vein Endothelial Cells/metabolism , Atherosclerosis/genetics , Atherosclerosis/metabolism , Integrins/metabolism , RNA/metabolism
3.
Int J Mol Sci ; 23(5)2022 Mar 07.
Article in English | MEDLINE | ID: mdl-35270021

ABSTRACT

Hyperglycemia is reported to accelerate endothelial cell senescence that contributes to diabetic complications. The underlying mechanism, however, remains elusive. We previously demonstrated AQR as a susceptibility gene for type 2 diabetes mellitus (T2DM) and showed that it was increased in multiple tissues in models with T2DM or metabolic syndrome. This study aimed to investigate the role of AQR in hyperglycemia-induced senescence and its underlying mechanism. Here, we retrieved several datasets of the aging models and found the expression of AQR was increased by high glucose and by aging across species, including C. elegans (whole-body), rat (cardiac tissues), and monkey (blood). we validated the increased AQR expression in senescent human umbilical vein endothelial cells (HUVECs). When overexpressed, AQR promoted the endothelial cell senescence, confirmed by an increased number of cells stained with senescence-associated beta-galactosidase and upregulation of CDKN1A (P21) as well as the prohibited cellular colony formation and G2/M phase arrest. To explore the mechanism by which AQR regulated the cellular senescence, transcriptomic analyses of HUVECs with the overexpression and knockdown of the AQR were performed. We identified 52 co-expressed genes that were enriched, in the terms of plasminogen activation, innate immunity, immunity, and antiviral defense. Among co-expressed genes, PLAU was selected to evaluate its contribution to senescence for its highest strength in the enrichment of the biological process. We demonstrated that the knockdown of PLAU rescued senescence-related phenotypes, endothelial cell activation, and inflammation in models induced by AQR or TNF-α. These findings, for the first time, indicate that AQR/PLAU is a critical signaling axis in the modulation of endothelial cell senescence, revealing a novel link between hyperglycemia and vascular dysfunction. The study may have implications in the prevention of premature vascular aging associated with T2DM.


Subject(s)
Biological Phenomena , Diabetes Mellitus, Type 2 , Hyperglycemia , Animals , Caenorhabditis elegans , Cells, Cultured , Cellular Senescence/genetics , Human Umbilical Vein Endothelial Cells/metabolism , Humans , Hyperglycemia/genetics , Hyperglycemia/metabolism , Rats
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