Molecular analysis of Boophilus spp. (Acari: Ixodidae) tick strains.

Boophilus spp. (Acari: Ixodidae) parasitize cattle and other farm and wild animals in tropical and subtropical regions of the world. Ticks belonging to the genus Boophilus have undergone evolutionary processes associated with habitat adaptation following biogeographical separation, resulting in strains with marked morphological differences. We have characterized at the molecular level B. microplus strains from Latin America and Australia, employing sequences derived from the bm86 coding region, an intron located within the bm86 gene, and DNA short tandem repeats (STR). A B. annulatus strain was employed for comparison. The results indicated that variation within the bm86 coding region is higher between B. microplus strains than between some B. microplus strains and B. annulatus. The sequence of the intron was not informative for phylogenetic analysis, varying among individuals of the same strain. Two STRs were identified in B. microplus (STRs BmM1 and BmM2) and one in B. annulatus (STR Ba1). Southern hybridization experiments with STRs BmM1 and BmM2 as a probe revealed the prevalence of dispersed moderately repeated DNA in the genome of B. microplus. The analysis of polymorphism at STR locus BmM1 evidenced differences within and between populations of B. microplus. These results support at the molecular level the existing differences between B. microplus strains and suggest tools to characterize these populations.

Sequence variations in the Boophilus microplus Bm86 locus and implications for immunoprotection in cattle vaccinated with this antigen.

Cattle tick infestations constitute a major problem for the cattle industry in tropical and subtropical regions of the world. Traditional control methods have been only partially successful, hampered by the selection of chemical-resistant tick populations. The Boophilus microplus Bm86 protein was isolated from tick gut epithelial cells and shown to induce a protective response against tick infestations in vaccinated cattle. Vaccine preparations including the recombinant Bm86 are used to control cattle tick infestations in the field as an alternative measure to reduce the losses produced by this ectoparasite. The principle for the immunological control of tick infestations relies on a polyclonal antibody response against the target antigen and, therefore, should be difficult to select for tick-resistant populations. However, sequence variations in the Bm86 locus, among other factors, could affect the effectiveness of Bm86-containing vaccines. In the present study we have addressed this issue, employing data obtained with B. microplus strains from Australia, Mexico, Cuba, Argentina and Venezuela. The results showed a tendency in the inverse correlation between the efficacy of the vaccination with Bm86 and the sequence variations in the Bm86 locus (R2 = 0.7). The mutation fixation index in the Bm86 locus was calculated and shown to be between 0.02 and 0.1 amino acids per year. Possible implications of these findings for the immunoprotection of cattle against tick infestations employing the Bm86 antigen are discussed.

The adduction distraction maneuver.

The adduction distraction maneuver is presented as an adjunct to the surgeon's technical skills to assist with the initial introduction of the shoulder arthroscope. Both novice and experienced arthroscopists can experience difficulty establishing access to the glenohumeral joint. Often this results in articular cartilage or soft tissue damage. The adduction distraction maneuver when used in the "beach chair" seated position for shoulder arthroscopy can facilitate posterior portal placement and minimize iatrogenic trauma.

College women who initiate assaults on their male partners and the reasons offered for such behavior.

Studies of spousal and dating violence indicate that women are as likely as men to assault their partners physically. This investigation examined the issue of the initiation of physical assaults by women on their male partners and the reasons offered for such behavior. Response from 978 female college women indicate that, within a 5-yr. period, 29% (n = 285) admitted to physical aggression against their male partners. Younger women in their 20's were significantly more likely to aggress physically than women who were 30 yr, and above. Women stated that they expressed aggression toward their male partners in part because they wished to engage their partner's attention, particularly emotionally. Also assaultive women did not believe that their male victims would be seriously injured or would retaliate.

Incidence and methods of diagnosis of musculoskeletal injuries incurred in Operations Desert Shield and Desert Storm.

RATIONALE AND OBJECTIVES: The authors studied the incidence and distribution of orthopedic injuries sustained in Operation Desert Shield and Desert Storm by evaluating whether existing diagnostic imaging modalities were sufficient to diagnose the types of musculoskeletal injuries incurred. The authors also sought to determine if a dedicated extremity MR scanner would provide monetary benefits and enhance military readiness. METHODS: The authors retrospectively reviewed data on all musculoskeletal injuries incurred in Desert Shield/Desert Storm to determine the total number of injuries, proportion of orthopedic injuries, number of soft-tissue orthopedic injuries, and the country where the diagnosis was established. The authors also determined the number of patients, duration, and economic impact of the evacuation process. RESULTS: There were 1011 fractures and 1177 soft-tissue injuries; 408 soft-tissue injuries were diagnosed in the Persian Gulf countries, and 769 diagnosed in the United States and Germany. The average time to evacuate these 769 patients was 21 days with an estimated replacement cost of $836,885. CONCLUSIONS: Musculoskeletal soft-tissue injuries comprised 34% of the overall injuries in the Persian Gulf War, and twice as many patients required evacuation for diagnosis as were diagnosed locally using existing imaging modalities. A dedicated extremity magnetic resonance scanner in the battlefield would obviate many evacuations and hence be of both monetary and military readiness benefit.