ABSTRACT
Some studies on neurodevelopmental outcomes after neonatal encephalopathy have suggested that cognitive deficits do not occur in the absence of cerebral palsy. It is increasingly apparent that childhood survivors of overt neonatal encephalopathy may have cognitive impairments, even in the absence of functional motor deficits. The risk of cognitive deficits is related to the severity of neonatal encephalopathy and the pattern of brain injury on neuroimaging, particularly the watershed pattern of injury. A better understanding of the risk factors for cognitive abnormalities after neonatal encephalopathy will ultimately lead to interventions to prevent these deficits. Identifying the full spectrum of neurodevelopmental outcomes after neonatal encephalopathy will also allow care givers to identify children requiring early intervention to maximise their potential for independent function throughout development.
Subject(s)
Asphyxia Neonatorum/psychology , Brain Diseases/etiology , Cognition Disorders/etiology , Animals , Biomarkers/metabolism , Cerebral Palsy , Humans , Infant, Newborn , Magnetic Resonance ImagingABSTRACT
The catalytic properties of expressed human cytochromes P4501A1, 1A2, 3A4 and 3A5 with respect to the metabolite distribution for the carcinogen benzo[a]pyrene (BP) and the proximate carcinogen (-)-trans-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene ((-)-BP-7,8-DHD) were determined. P4501A1 formed a higher proportion of the proximate carcinogen from BP than the other isozymes. Both P4501A1 and 1A2 were more selective for oxidation at the benzo ring of BP and showed a greater production of dihydrodiol metabolites than either P4503A4 or 3A5. The formation of diolepoxides from the individual enantiomers of BP-7,8-DHD occurred in a highly stereoselective fashion.
Subject(s)
7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide/metabolism , Benzo(a)pyrene/metabolism , Cytochrome P-450 Enzyme System/metabolism , Isoenzymes/metabolism , Animals , Cell Line , Cloning, Molecular , Cytochrome P-450 Enzyme System/genetics , Humans , Isoenzymes/genetics , Isomerism , Kinetics , Substrate Specificity , TransfectionABSTRACT
In Macaca sylvana restrained in chairs, continuous 24 h recordings were obtained of scalp EEG, neck EMG, bilateral EOG, motility of the head and lever manipulation. A total of 32 days were studied in 8 monkeys. Recordings were divided into epochs of 30 sec and analyzed in a PDP-12 computer. Four stages of sleep and 5 stages of wakefulness were identified by combination of the above data. Results were as follows: (1) During the night, there was 21% wakefulness and during the day 20% sleep, including a considerable amount of REM. (2) Recordings of spontaneous motility of head and hands proved an excellent indicator of sleep-wakefulness cycles. (3) Spectral analysis revealed the cyclic characteristics of non-REM sleep, with peaks at 360, 149, 72, and 55 min. (4) Stages of wakefulness had ultradian cycles, and stage W-2 was the most important in number and duration of occurrences. (5) Night wakefulness had characteristics different from day wakefulness. (6) Study of sequences showed that stage II was the least differentiated being transitional among the other stages, while stage III-IV was never preceded by wakefulness or REM, and almost always it followed stage II (99.0% of the time). (7) The high statistical significance of results obtained under restraint in different monkeys suggests that this situation has great potential value in the investigation of physiological mechanisms of ultradian rhythms.
