ABSTRACT
Genetic studies in human and mice have established a dual role for Vsx genes in retina development: an early function in progenitors' specification, and a later requirement for bipolar-cells fate determination. Despite their conserved expression patterns, it is currently unclear to which extent Vsx functions are also conserved across vertebrates, as mutant models are available only in mammals. To gain insight into vsx function in teleosts, we have generated vsx1 and vsx2 CRISPR/Cas9 double knockouts (vsxKO) in zebrafish. Our electrophysiological and histological analyses indicate severe visual impairment and bipolar cells depletion in vsxKO larvae, with retinal precursors being rerouted toward photoreceptor or Müller glia fates. Surprisingly, neural retina is properly specified and maintained in mutant embryos, which do not display microphthalmia. We show that although important cis-regulatory remodelling occurs in vsxKO retinas during early specification, this has little impact at a transcriptomic level. Our observations point to genetic redundancy as an important mechanism sustaining the integrity of the retinal specification network, and to Vsx genes regulatory weight varying substantially among vertebrate species.
Subject(s)
Homeodomain Proteins , Zebrafish , Animals , Humans , Mice , Zebrafish/genetics , Zebrafish/metabolism , Homeodomain Proteins/metabolism , Retina/metabolism , Genes, Homeobox , Zebrafish Proteins/genetics , Zebrafish Proteins/metabolism , Mutation , Mammals/genetics , Transcription Factors/metabolism , Eye Proteins/metabolismABSTRACT
The provision of high data rate services to mobile users combined with improved quality of experience (i.e., zero latency multimedia content) drives technological evolution towards the design and implementation of fifth generation (5G) broadband wireless networks. To this end, a dynamic network design approach is adopted whereby network topology is configured according to service demands. In parallel, many private companies are interested in developing their own 5G networks, also referred to as non-public networks (NPNs), since this deployment is expected to leverage holistic production monitoring and support critical applications. In this context, this paper introduces a 5G NPN architectural approach, supporting among others various key enabling technologies, such as cell densification, disaggregated RAN with open interfaces, edge computing, and AI/ML-based network optimization. In the same framework, potential applications of our proposed approach in real world scenarios (e.g., support of mission critical services and computer vision analytics for emergencies) are described. Finally, scalability issues are also highlighted since a deployment framework of our architectural design in an additional real-world scenario related to Industry 4.0 (smart manufacturing) is also analyzed.
Subject(s)
Social Networking , Wireless Technology , Multimedia , TechnologyABSTRACT
Se presenta el caso de una mujer de 41 años de edad con crecimiento uterino, debido a la presencia de tumores de estirpe muscular lisa, muchos de ellos microscópicos, y con proliferación de células miógenas alrededor de vasos sanguíneos. Los datos anteriores conforman el cuadro morfológico de leiomiomatosis difusa. Se comenta la patogénesis del padecimiento y el diagnóstico diferencial con sarcoma del estroma endometrial de bajo grado y la adenomiosis.
Subject(s)
Humans , Adult , Female , Leiomyomatosis , Hysterectomy , Sarcoma, Endometrial Stromal , Diagnosis, Differential , Histological TechniquesABSTRACT
Se informa sobre la realización de la primera esplenectomía en México por vía laparoscópica en una mujer de 32 años de edad con enfermedad de base anemia hemolítica autoinmune así como colecistectomía laparoscópica incidental en octubre de 1992 en el Hospital General de México, S.S.A. Su evolución fue satisfactoria al utilizar menos sangre para transfusión, el dolor postoperatorio mínimo, dándose de alta a la paciente a la 72 hrs. estando en la actualidad asintomática y bajo seguimiento por el Servicio de Hematología
