Effects of Carpobrotus edulis invasion on soil gross N fluxes in rocky coastal habitats.

The effects of alien plants on whole nutrient cycles have been scarcely studied, despite the increasing evidence on their impact on nutrient pools and fluxes. Carpobrotus edulis, a dangerous invasive plant in coastal areas worldwide, is considered an ecosystems engineer which, by changing many soil properties, benefits its own invasion and hampers the restoration of the invaded habitats. To study, for the first time, the 'true' impact of C. edulis on the soil N cycle, we used a paired 15N labelling experiment and a Ntrace compartment model to estimate the gross N fluxes in the 0-5cm and 5-10cm soil layers of non-invaded and C. edulis invaded areas of two temperate-humid coastal rocky locations. Carpobrotus edulis invasion generally increased NH4+ immobilization (INH4, 1.19-4.48×), presumably due to a lower N availability for the microbiota. The invasion also decreased autotrophic nitrification (ONH4, 0.20-0.79×), either by a direct effect over soil microbiota or by the acidification triggered by C. edulis. Unexpectedly, the dissimilatory nitrate reduction (DNRA) was the exclusive NO3- consuming process modelled on most of the studied soils, although the incubation was aerobic. Apparently, the high organic C content of these soils induced a higher O2 consumption and the formation of anaerobic microsites where the DNRA could have taken place. The lower NO3- availability of invaded soils could explain their lower DNRA rates (0.04-0.70×) compared to native soils. Both DNRA and ONH4 were more affected in the 0-5cm layer, but the invasion also significantly affected N rates in the 5-10cm layer. Overall, net nitrification and mineralization generally decreased in the invaded soils. This study shows that the invasion of C. edulis alters soil gross and net N fluxes in a 0-10cm depth through its effects on soil properties and microbiota.

Partitioning CO2 effluxes from an Atlantic pine forest soil between endogenous soil organic matter and recently incorporated 13C-enriched plant material.

Soil CO2 effluxes from recently added 13C-labeled phytomass versus endogenous soil organic matter (SOM) were studied in an acid soil from Atlantic pine forests (NW Spain). After several cultures to incorporate fresh 13C-enriched Lolium perenne to a Humic Cambisol with predominance of humus--Al over humus--Fe complexes, potential soil C mineralization was determined by laboratory aerobic incubation (84 days). Isotopic 13C analyses of SOM fractions were assessed to know in which organic compartments the 13C was preferentially incorporated. Although in the 13C-labeled soil the C mineralization coefficient totalized less than 3% of soil C, the 13C mineralization coefficient exceeded 14%, indicating a greater lability of the newly incorporated organic matter. Organic compounds coming from added phytomass showed a higher lability and contributed considerably to the total soil CO2 effluxes (52% of total soil CO2 evolved during the first decomposition stages and 27% at the end), even though added-C comprised less than 4% of total soil C. Good determination coefficients, when values of CO2--C released were fitted to a first-order double exponential kinetic model, support the existence of two C pools of different lability. Kinetic parameters obtained with this model indicated that phytomass addition augmented the biodegradability of the labile pool (instantaneous mineralization rate k increased from 0.07 d(-1) to 0.12 d(-1)) but diminished that of the recalcitrant pool (instantaneous mineralization rate h decreased from 2.7 x 10(-4) d(-1) to 1.6 x 10(-4) d(-1)). Consequently, the differentiation between both SOM pools increased, showing the importance of SOM quality on CO2 emissions from this kind of soil to the atmosphere.

13C-isotopic fingerprint of Pinus pinaster Ait. and Pinus sylvestris L. wood related to the quality of standing tree mass in forests from NW Spain.

Pine forest plantations of Pinus pinaster Ait. and P. sylvestris L. located in Galicia, NW Spain, were selected to study the 13C/12C-isotopic fingerprint in wood core samples in order to find possible relationships between the delta(13)C at natural abundance levels and the quality of the standing tree mass. For each pine species, 24 forests growing on acidic soils were studied: half developed over granite and half over schists. Two dominant trees from each plot, corresponding to all possible combinations of forest stands with high or low site index and with adults or young trees, were drilled at the basal part of trunks using a Pressler drill to obtain tree ring samples. The C-isotopic compositions of the litter and the soil organic matter from different soil depths were also determined and statistically significant correlations between these values and the 13C content of the wood were observed. Despite internal variations due to the influence of site index, tree age and parent material, the isotopic fingerprint of P. pinaster wood (mean value delta13C=-26.2+/-0.8 per thousand) significantly differed (P<0.001) from that of P. sylvestris (mean value delta13C=-24.6+/-0.7 per thousand). Relationships between the quality of the stand and the C-isotopic composition of the wood were observed, high quality stands having trees more 13C-depleted than low quality ones. A high correlation between wood delta13C and site index values for P. pinaster stands (r=-0.667, P<0.001) was found, this correlation being even clearer when only P. pinaster growing over schists (r=-0.833, P<0.001) are considered. Again, the correlation between the site index and the wood delta13C of young P. pinaster trees is higher when plots over granite or schists are separately considered. A similar fact occurs for adult P. sylvestris trees from schists stands, high quality specimens being 13C-depleted compared with low quality ones. On the other hand, 13C natural abundance of wood from P. sylvestris trees seems to be also strongly influenced by the underlying parent material, young trees from granite stands having a statistically higher 13C-isotopic composition (P<0.05) than young trees from schists stands.

Use of 13C to monitor soil organic matter transformations caused by a simulated forest fire.

Soil organic matter (SOM) transformations caused by heating were analyzed using the stable carbon isotope (13)C as a tracer to follow C mineralization dynamics and C transfers between different organic compartments. A (13)C-labelled soil, obtained by incorporation of (13)C-enriched Lolium perenne phytomass into a pine forest soil, was heated for 10 min at 385 degrees C to reproduce conditions typical of a forest fire and changes in total C content, potential C mineralization activity and C distribution between the different soil organic fractions were determined. Changes caused by heating on the potential soil C mineralization, determined by laboratory aerobic incubation, reveal alterations to the SOM biodegradability; some stabilized SOM showed an increase in biodegradability, whereas less stabilized SOM became more resistant to microorganisms. Chemical fractionations of SOM allowed us to monitor changes in its composition. As a consequence of heating, the less polymerized humic fractions were the most strongly affected, with the total disappearance of fulvic acids. A significant increase in the quantity and degree of polymerization of the humic acids at the expense of other more (13)C-enriched substances was also found. Finally, a large decrease in humin was observed, its solubilizable part disappearing completely, probably as a consequence of the incorporation of the byproducts into the free organic matter fraction.