Origin, Selection and Current Status of the Utrerana Chicken Breed: A Review.

The conservation of threatened local livestock genetic resources involves characterising them to implement conservation strategies. The Utrerana is a Mediterranean-type chicken breed, included in the Official Catalogue of Livestock Breeds of Spain and in the Domestic Animal Diversity Information System (DAD-IS) of the Food and Agriculture Organization (FAO), native to south Spain created in 1926 by a farmer from Utrera (Seville province). It was selected for laying performance and with four plumage varieties (White, Black, Black-barred and Partridge), reaching average yields of 180 eggs per hen per year. It was widely used in commercial farming in the second quarter of the 20th century, being subsequently displaced in the second half of the 20th century by the spreading of the improved foreign breeds and lines. The Utrerana breed was reared from then on for its aesthetic values and in backyard systems, being endangered with a vulnerable local risk status (1822 birds in 2022 with an increasing trend) and having worsened its laying performance as selection for productivity ceased. The breed has received little attention from the research community. Therefore, this work aims to review the literature on the origin, selection, genetic and productive characterisation and status of the populations of the breed, as well as the conservation strategies. The Utrerana chicken is a polymorphic breed showing high genetic diversity, sexual dimorphism and morpho-functional differences among varieties. Currently, Utrerana hens start laying at 6 months of age, and a hen lays 94-121 high quality eggs (59-64 g) per year, showing seasonality. It is a slow-growing breed with mature weights of 2.4-2.6 kg for roosters and 1.9-2.0 g for hens. The Utrerana chicken breed is rustic and adapted to alternative farming systems. This review has identified research gaps to be filled, such as characterising the carcass and meat quality of the Utrerana chicken, and evidences the need to make efforts to promote the breed and expand its populations.

Effects of Long-Term Storage on Hatchability and Incubation Length of Game Farmed Quail Eggs.

The long-term storage of eggs before incubation is a common practice in some alternative poultry systems but needs to be performed under conditions that preserve egg viability. The effects of the long-term storage of game farmed quail (Coturnix coturnix) eggs on weight loss during the storage and incubation periods, chick weight at hatch, hatchability, and incubation length were investigated. The eggs were arranged in six treatments submitted to 0-, 7-, 14-, 21-, 28-, and 35-day storage periods at 15.8 °C and 80% relative humidity. The storage length reduced the hatchability of eggs (p < 0.05) when the storage was extended to 35 days, decreasing by more than half compared to eggs stored up to 28 days. Egg weight loss during storage progressively increased with the storage length (p < 0.05). Chick weight at hatching was reduced in eggs stored for more than 14 days (p < 0.05), and relative chick weight decreased significantly in eggs stored for 35 days (p < 0.05). Incubation length progressively increased with the storage length (p < 0.05), achieving less hatching synchrony in eggs stored for a longer time (p < 0.05). In conclusion, game quail eggs store well with little deterioration up to 28 days at 15.8 °C and 80% relative humidity, allowing for extended storage when shipping long-shelf-life eggs or assembling batches large enough to fully set an incubator in farms with small breeding flocks.

The Quail Game Farming Sector in Spain.

Quail (Coturnix genus) game farms were characterised in Spain using variables related to their age, geographical distribution, farmed species, structure, size, and commercialisation, using a survey conducted on 21 farms in 2018. It was found that 38.1% of the sample represented complete-cycle farms, and 61.9%, which have no breeding flocks, undertook only the finishing phase of quail raising. The average size of the breeding flock, with regard to complete-cycle farms, was 1096 males and 3735 females, with a female/male ratio of 3.6, and 75% of these farms carried out self-replacement of breeding quails. The most farmed species was European quail (Coturnix coturnix), followed by Japanese quail (Coturnix japonica), and hybrids of both species. In addition to quail, all farms produced other gamebird species such as pheasant (Phasianus colchicus) and red-legged partridge (Alectoris rufa). The rearing phase, which began when the chicks were one-day-old, lasted 35.3 days on average (range: 25-49 days), with an average stocking density of 47.2 birds/m2 in the brooder house. The finishing phase, which was carried out in flying pens at a stocking density of 9.5 birds/m2, ended when the quails' average age was 60.5 days. All farms sold quails for release in hunting reserves (EUR 1.54 per bird) and for dog training (EUR 1.65 per bird) for almost 10 months of the year; only 62% sold quails for shooting after being thrown with an adapted clay-pigeon shooting machine (EUR 1.49 per bird). Transporting quails to their destination (95.2% of the farms) and releasing the birds in the client's hunting preserve (52.4% of the farms) were services offered by the farms, among others. The main advertising and promotional strategies undertaken by the quail game farms to gain a share of the market included maintaining a business website (85.7% of the farms) and attending game and agricultural fairs (47.6% of the farms). In conclusion, this alternative poultry sector has been consolidated in Spain, five decades since the establishment of the first game farms, and it satisfies a relevant part of the demand for quail hunting.

Influence of Stress Assessed through Infrared Thermography and Environmental Parameters on the Performance of Fattening Rabbits.

Rabbits often experience stress when they perceive certain stimuli, such as handling. The physiological response of animals to stress and temperament is associated with feeding efficiency, with those with the least capacity to react to stress showing the highest performance. The aim of this study was to evaluate the impact of stress due to heat (temperature-humidity index; THI) or handling, assessed using infrared thermography, on the performance parameters of rabbits of a Spanish Common breed. Thirty-nine rabbits were analyzed during a 38-d fattening period at two times of the year: a cold period and a warm period. The rabbits' stress due to handling was assessed by the temperature difference taken in the inner ear, before and after being handled. The animals were more productive in the cold season. Temperature-humidity index significantly influenced average daily gain (ADG) and daily feed intake (DFI). Rabbits with higher levels of stress showed higher ADG, DFI and feed conversion ratio (FCR) values. The greater the stress due to handling, the less efficient the animals were. FCR increased with higher THI. It was concluded that changes in animal welfare caused by the rabbits' reactivity to both climatic and individual factors affect animal productivity.

Hypolipidemic and Hypoglycaemic Effect of Wholemeal Bread with Amaranth (Amaranthus dubius Mart. ex Thell.) on Sprague Dawley Rats.

The effect of consuming breads made with wheat flour and Amaranth (Amaranthus dubius Mart. ex Thell.) wholemeal flour on Sprague Dawley rats with hyperlipidaemia and hyperglycaemia induced through feeding was studied. Four diets were provided: control (CD: Ratarina®), commercial bread (CBD), bread with 100 g·kg-1 (ABD10) and 200 g·kg-1 (ABD20) amaranth flour. Zoometric and blood chemistry parameters were measured before and after consuming the diets. A completely random factorial design of 2 × 4 × 2 was used. The factors were blood lipids and glucose level (normal, N and elevated, E), diet (CD, CBD, ABD10 and ABD20) and sex (female, F and male, M). The rats consuming ABD10 and ABD20 diets presented the lowest glucose values, although with no differences (p > 0.05) between the groups of elevated blood lipids and glucose rats (E). Triglyceride concentrations decreased in ABD10 and ABD20 treatments in comparison with CD, elevated blood lipids and glucose (E) rats, while ABD10 rats showed lower total cholesterol level than normal (N) rats. The high-density lipoprotein cholesterol values increased in the ABD10 and ABD20 groups (p < 0.05), while it did lower for very low-density lipoprotein cholesterol and cardiac risk index (p < 0.05). In ABD10 and ABD20 treatments, the abdominal circumference decreased in both sexes (p < 0.05) between weeks 23 and 31. In conclusion, consumption of bread with amaranth improved lipid profiles of rats and could help to prevent metabolic and cardiovascular diseases.

Stability of fatty acid composition of intramuscular fat from pasture- and grain-fed young bulls during the first 7 d postmortem.

In order to study the effect of different amounts of concentrate feed and the effectiveness of natural antioxidants on the fatty acid stability of intramuscular fat during the first days postmortem, 75 young bulls of the Retinta breed were divided in three groups: 30 were grazed, 30 were fed on medium concentrate diets, and 15 were fed on high-concentrate diets. Young bulls were slaughtered at commercial weight, around a 500 kg final body weight. Samples from Longissimus lumborum muscle were assigned to two ageing periods (0 and 7 d) and were vacuum packaged in vacuum bags ( O 2 permeability: 9.3  mL   O 2 / m 2 per 24  h at 0  ∘ C ) using an EGARVAC ® sealer. Beef from grass-fed bulls showed a higher polyunsaturated fatty acid content than concentrate-fed bulls. During the first 7 d postmortem, no changes in the fatty acids profile were observed, because α -tocopherol content was optimal to prevent lipid oxidation. The higher level of natural antioxidants in grass than in grain resulted in the stability of the fatty acid profile. This study shows that the anti-oxidative potential of natural antioxidants in meat plays an important role during the first 7 d postmortem.

Genetic variation at the goat hormone-sensitive lipase ( LIPE) gene and its association with milk yield and composition.

Hormone-sensitive lipase (LIPE) plays a fundamental role in the regulation of energy balance by releasing free fatty acids from adipose triacylglycerol stores. These fatty acids can be subsequently transferred to other body compartments to be oxidized or employed in other biochemical reactions. This enzymic function is particularly important in lactating animals because the synthesis of milk components involves the mobilization of lipid depots to satisfy the large energy demands of the mammary gland. In the current study, we partially sequenced the goat LIPE gene in several individuals. In doing so, we identified two synonymous polymorphisms at exons 2 (c.327C>A>T, triallelic polymorphism) and 3 (c.558C>T). Moreover, we found a mis-sense polymorphism at exon 6 (c.1162G>T) that involves an alanine to serine substitution at position 388. Analysis with Polyphen and Panther softwares revealed that this amino acid replacement is expected to be neutral. Performance of an association analysis with a variety of milk traits revealed that goat LIPE genotype has highly suggestive effects on milk yield (P=0.0032) as well as on C18:3 n-6g (P=0.0051), trans-10 cis-12 CLA (P=0.007) and C12:0 (P=0.0084) milk contents. These associations are concordant with the preference of LIPE to selectively mobilize medium-chain and unsaturated fatty acids.

Factors affecting rabbit meat consumption among Spanish university students.

This study investigates the rabbit meat consumption patterns among students. Results of the 342 students surveyed at the University of Seville, Spain showed that more men than women consumed rabbit meat. More students consumed wild rabbit meat than farmed rabbit meat. The practice of hunting by students or their relatives, and the raising of rabbits for self-consumption, positively influenced the proportion of students that currently consumed rabbit meat. Most of the students that had eaten rabbit meat before but are currently non-eaters do so because of the meat's organoleptic characteristics or from lack of consumption habit.